BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120526.Seq (685 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 116 4e-27 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 88 1e-18 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 84 2e-17 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 73 5e-14 SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 58 9e-10 SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 54 2e-08 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 53 3e-08 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 29 0.47 SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 28 1.4 SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 27 1.9 SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schiz... 27 3.3 SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 26 4.4 SPAPB1A11.01 ||SPAPB24D3.11|membrane transporter|Schizosaccharom... 26 5.8 SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosac... 26 5.8 SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce... 25 7.7 SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 25 7.7 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 116 bits (278), Expect = 4e-27 Identities = 58/100 (58%), Positives = 74/100 (74%), Gaps = 1/100 (1%) Frame = +1 Query: 244 GNGSDYRLLVTQARKMAQQ*FL-MYHEPIPTAQLVQHVATVMQEYTQSGGVRPFGVSLLI 420 G G D+R+LV ++RK+AQ + +Y+E PT LVQ +A+VMQE TQSGGVRPFGVSLL+ Sbjct: 77 GMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGGVRPFGVSLLV 136 Query: 421 CGWEEGRPYLFQCDPSGAYFAWKATAMGKTLIMEKLSLKK 540 G +E P L+Q DPSG YFAWKATA+GK+ K L+K Sbjct: 137 AGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEK 176 Score = 99 bits (238), Expect = 3e-22 Identities = 53/78 (67%), Positives = 62/78 (79%) Frame = +2 Query: 20 SERYSFSLTTFSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKSILYDEHSV 199 +++Y+FSLTTFSP+GKLVQIEYAL AV AG TSVGIKA++GVV+ATE K S L S+ Sbjct: 2 TDKYAFSLTTFSPNGKLVQIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGASL 61 Query: 200 NKVEMITGHIGMVYSGMG 253 KV IT IGMVYSGMG Sbjct: 62 EKVCAITPDIGMVYSGMG 79 Score = 56.8 bits (131), Expect = 3e-09 Identities = 27/47 (57%), Positives = 33/47 (70%) Frame = +3 Query: 462 PLRSLFCMESHSHGKDFNNGKTFLEKRYTEDLELDDAVHTAILTLKE 602 P + F ++ + GK KTFLEKRY ++LELDDAVHTAIL LKE Sbjct: 151 PSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDDAVHTAILALKE 197 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 88.2 bits (209), Expect = 1e-18 Identities = 46/102 (45%), Positives = 66/102 (64%), Gaps = 2/102 (1%) Frame = +1 Query: 244 GNGSDYRLLVTQARKMAQQ*FLMYHEPIPTAQLVQHVATVMQEYTQSGGVRPFGVSLLIC 423 G +D R+L+ +AR AQ L +P+ L ++VA V Q+YTQSGGVRPFGVS LI Sbjct: 76 GLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIA 135 Query: 424 GWEEG--RPYLFQCDPSGAYFAWKATAMGKTLIMEKLSLKKD 543 G++ G P ++Q +P+G Y AWKATA+G+ + L+K+ Sbjct: 136 GFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKN 177 Score = 56.8 bits (131), Expect = 3e-09 Identities = 23/74 (31%), Positives = 44/74 (59%) Frame = +2 Query: 29 YSFSLTTFSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKSILYDEHSVNKV 208 Y +L+ FSP G+L+Q+EY AV G T++ ++ + +VI E K+ L + + K+ Sbjct: 4 YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQKI 63 Query: 209 EMITGHIGMVYSGM 250 M+ H+ + ++G+ Sbjct: 64 AMVDNHVCLAFAGL 77 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 84.2 bits (199), Expect = 2e-17 Identities = 40/88 (45%), Positives = 57/88 (64%), Gaps = 1/88 (1%) Frame = +1 Query: 244 GNGSDYRLLVTQARKMAQQ*FLMYHEPIPTAQLVQHVATVMQEYTQSGGVRPFGVSLLIC 423 G +D +L+ AR++ QQ ++E +P QLV+ V + Q YTQ GG+RPFGVS L Sbjct: 78 GLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYA 137 Query: 424 GWEEGRPY-LFQCDPSGAYFAWKATAMG 504 GW+ R Y LFQ +PSG Y +W+A ++G Sbjct: 138 GWDHIRGYQLFQSNPSGNYGSWQANSIG 165 Score = 55.6 bits (128), Expect = 6e-09 Identities = 30/78 (38%), Positives = 44/78 (56%), Gaps = 1/78 (1%) Frame = +2 Query: 20 SERYSFSLTTFSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKS-ILYDEHS 196 S Y T FSP G+L Q+EYAL A+ G ++GI A +G+V+A E K S +L E S Sbjct: 2 SRSYDSRTTIFSPEGRLYQVEYALEAINHAGVALGIVAKDGIVLAAEKKVTSKLLEQEES 61 Query: 197 VNKVEMITGHIGMVYSGM 250 K+ I ++ +G+ Sbjct: 62 AEKLYHIGDNMLCAVAGL 79 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 72.5 bits (170), Expect = 5e-14 Identities = 32/81 (39%), Positives = 47/81 (58%) Frame = +2 Query: 8 VIMASERYSFSLTTFSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKSILYD 187 + M Y + TFSP G+L Q+EYA+ A+ G T++G+K + VV+ E + S L + Sbjct: 1 MFMTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLME 60 Query: 188 EHSVNKVEMITGHIGMVYSGM 250 HSV K+ I HIG SG+ Sbjct: 61 SHSVEKLFEIDSHIGCAISGL 81 Score = 53.6 bits (123), Expect = 3e-08 Identities = 34/109 (31%), Positives = 49/109 (44%), Gaps = 6/109 (5%) Frame = +1 Query: 244 GNGSDYRLLVTQARKMAQQ*FLMYHEPIPTAQLVQHVATVMQEYTQSGG------VRPFG 405 G +D R ++ AR Q Y EP Q + + + + RPFG Sbjct: 80 GLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFG 139 Query: 406 VSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKTLIMEKLSLKKDIQK 552 V+LLI G +E P L+ +PSG YF ++A A+G K L K+ K Sbjct: 140 VALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHK 188 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 58.4 bits (135), Expect = 9e-10 Identities = 30/89 (33%), Positives = 46/89 (51%) Frame = +1 Query: 238 LLGNGSDYRLLVTQARKMAQQ*FLMYHEPIPTAQLVQHVATVMQEYTQSGGVRPFGVSLL 417 + G D R+L ++ A ++ PIP +L+ VA Q TQ G RP+GV L Sbjct: 74 IAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFL 133 Query: 418 ICGWEEGRPYLFQCDPSGAYFAWKATAMG 504 + G++E P+L + PSG + T+MG Sbjct: 134 VIGYDESGPHLLEFQPSGLVLEYLGTSMG 162 Score = 46.4 bits (105), Expect = 4e-06 Identities = 25/79 (31%), Positives = 40/79 (50%) Frame = +2 Query: 14 MASERYSFSLTTFSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKSILYDEH 193 M +Y TT+SP G+L Q+EYAL A+ G +VG+ + V+ ++ L Sbjct: 1 MFRNQYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQ 60 Query: 194 SVNKVEMITGHIGMVYSGM 250 K+ I HIG+ +G+ Sbjct: 61 --KKLIRIDDHIGIAIAGL 77 >SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 54.0 bits (124), Expect = 2e-08 Identities = 31/90 (34%), Positives = 50/90 (55%), Gaps = 1/90 (1%) Frame = +1 Query: 238 LLGNGSDYRLLVTQARKMAQQ*FLMYHEPIPTAQLVQHVATVMQEYTQSGGVRPFGVSL- 414 + G+ +D R V++AR A + P+P L + +A + Q TQ +RP GV++ Sbjct: 78 MTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMT 137 Query: 415 LICGWEEGRPYLFQCDPSGAYFAWKATAMG 504 L+ +E P LF+ DP+G Y +KAT+ G Sbjct: 138 LVAVDDEIGPSLFKLDPAGFYIGYKATSAG 167 Score = 50.4 bits (115), Expect = 2e-07 Identities = 29/71 (40%), Positives = 39/71 (54%), Gaps = 1/71 (1%) Frame = +2 Query: 38 SLTTFSPSGKLVQIEYALAAV-AAGGTSVGIKASNGVVIATENKHKSILYDEHSVNKVEM 214 ++T FSP G+L Q+EYA A AG TSVG+ N + ++ K L D +V V Sbjct: 10 TITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFP 69 Query: 215 ITGHIGMVYSG 247 IT IG V +G Sbjct: 70 ITKGIGCVMTG 80 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 53.2 bits (122), Expect = 3e-08 Identities = 30/75 (40%), Positives = 40/75 (53%), Gaps = 3/75 (4%) Frame = +2 Query: 50 FSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKSILYDEHSVNKVEMITGHI 229 FSP G+L Q EYA AV T +GIK +GV++A E S L N++ + HI Sbjct: 14 FSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHI 73 Query: 230 GMVYSGM---GQTIV 265 G+ +G GQ IV Sbjct: 74 GIATTGFIPDGQHIV 88 Score = 51.2 bits (117), Expect = 1e-07 Identities = 27/95 (28%), Positives = 46/95 (48%) Frame = +1 Query: 256 DYRLLVTQARKMAQQ*FLMYHEPIPTAQLVQHVATVMQEYTQSGGVRPFGVSLLICGWEE 435 D + +V +AR A Y PIP + + +Q +T VRPFGV + ++ Sbjct: 83 DGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDS 142 Query: 436 GRPYLFQCDPSGAYFAWKATAMGKTLIMEKLSLKK 540 P+L+ +P+G Y+ + A GK + + L+K Sbjct: 143 EGPHLYMVEPNGVYWGYNGAAAGKGRQVARNELEK 177 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 29.5 bits (63), Expect = 0.47 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = -2 Query: 234 MPMWPVIISTLLTLCSSYSM 175 +P+WP+++S LL + SY M Sbjct: 1508 LPLWPIVVSELLIIFKSYCM 1527 >SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 27.9 bits (59), Expect = 1.4 Identities = 19/76 (25%), Positives = 31/76 (40%), Gaps = 4/76 (5%) Frame = +1 Query: 343 VQHVATVMQEYTQSGGVRPFGVSLL----ICGWEEGRPYLFQCDPSGAYFAWKATAMGKT 510 V + A + + YT + L + W P L P GA+ + +GK Sbjct: 202 VVNTADISENYTFDAPANELYATFLDPARVAAWSRAPPQL-DVRPQGAFSLFHGNVVGKF 260 Query: 511 LIMEKLSLKKDIQKTW 558 L++E+ K I +TW Sbjct: 261 LVLEE---NKKIVQTW 273 >SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 404 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = -1 Query: 331 SGSAHDTSRITAGPSSLLESPVDDSLTHSRVNHANV 224 S ++H S + GP S ++ S THS +H NV Sbjct: 17 SNNSHGDSFVNEGPPSNFKNNSLTSSTHSSTDHVNV 52 >SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 547 Score = 26.6 bits (56), Expect = 3.3 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = -2 Query: 222 PVIISTLLTLCSSYSMLLCLFSVAITTPFDALMPTEVPPAA 100 P +++T CSS L L + P A +P + PP A Sbjct: 265 PTLLNTYGNRCSSTDTLSSLSRLTSQDPLKASLPLQSPPLA 305 >SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual Length = 2410 Score = 26.2 bits (55), Expect = 4.4 Identities = 7/30 (23%), Positives = 18/30 (60%) Frame = -2 Query: 228 MWPVIISTLLTLCSSYSMLLCLFSVAITTP 139 +W +++ L + ++S ++C+F V + P Sbjct: 2245 LWALLLGVLAWISRTHSWIICVFGVGLGAP 2274 >SPAPB1A11.01 ||SPAPB24D3.11|membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 25.8 bits (54), Expect = 5.8 Identities = 8/29 (27%), Positives = 17/29 (58%) Frame = -2 Query: 225 WPVIISTLLTLCSSYSMLLCLFSVAITTP 139 WP+ I + T+ S+ +LC++ ++ P Sbjct: 33 WPIRIRIINTIIISFMTMLCMYGSSVFMP 61 >SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 25.8 bits (54), Expect = 5.8 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = +3 Query: 486 ESHSHGKDFNNGKTFLEKRYTEDLELDDAV 575 E ++ G DF + KTF TE+ E D+ + Sbjct: 140 EQNTDGSDFPSMKTFFNVMNTEEQEADEVM 169 >SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 25.4 bits (53), Expect = 7.7 Identities = 8/16 (50%), Positives = 13/16 (81%) Frame = -3 Query: 125 CRRKYHRRLRQLKHIR 78 C+R +HR+ Q++HIR Sbjct: 9 CKRVFHRQEHQVRHIR 24 >SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 793 Score = 25.4 bits (53), Expect = 7.7 Identities = 8/31 (25%), Positives = 18/31 (58%) Frame = -1 Query: 304 ITAGPSSLLESPVDDSLTHSRVNHANVASYH 212 + + PS++ SP D + ++N + + +YH Sbjct: 224 VLSSPSTIWWSPDSDKIAFLKLNESEIPTYH 254 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,978,425 Number of Sequences: 5004 Number of extensions: 63377 Number of successful extensions: 180 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 164 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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