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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120513.Seq
         (624 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati...   103   1e-22
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2...    99   1e-21
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1...    99   1e-21
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1...    99   1e-21
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati...    98   4e-21

>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 2 (Methionine
           adenosyltransferase 2, AdoMet synthetase 2)
           [Catharanthus roseus] SWISS-PROT:Q96552
          Length = 393

 Score =  103 bits (247), Expect = 1e-22
 Identities = 48/86 (55%), Positives = 65/86 (75%), Gaps = 2/86 (2%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KANVDY+++VR+T + IG+  +  G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGAGDQGLMFGYAT 507
           GVH +  +  EEVGAGDQG MFGYAT
Sbjct: 106 GVHGHLTKKPEEVGAGDQGHMFGYAT 131



 Score = 81.4 bits (192), Expect = 4e-16
 Identities = 42/70 (60%), Positives = 49/70 (70%), Gaps = 4/70 (5%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACE--TKLKPVWCF--CVAKSHP 298
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACE  TK   V  F     K++ 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63

Query: 299 KLTWIIKKLC 328
               I++K C
Sbjct: 64  DYEQIVRKTC 73



 Score = 36.7 bits (81), Expect = 0.013
 Identities = 17/27 (62%), Positives = 20/27 (74%)
 Frame = +3

Query: 510 ETEECMPLTVVLAHKLNPKIAELRRNG 590
           ET E MPLT VLA KL  K+ E+R+NG
Sbjct: 133 ETPELMPLTHVLATKLGAKLTEVRKNG 159


>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
           identical to S-adenosylmethionine synthetase 2
           (Methionine adenosyltransferase 2, AdoMet synthetase 2)
           [Arabidopsis thaliana] SWISS-PROT:P17562
          Length = 393

 Score =   99 bits (238), Expect = 1e-21
 Identities = 45/86 (52%), Positives = 63/86 (73%), Gaps = 2/86 (2%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KA +DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGAGDQGLMFGYAT 507
           GVH +  +  E++GAGDQG MFGYAT
Sbjct: 106 GVHGHFTKRPEDIGAGDQGHMFGYAT 131



 Score = 81.0 bits (191), Expect = 6e-16
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47



 Score = 33.1 bits (72), Expect = 0.15
 Identities = 14/27 (51%), Positives = 20/27 (74%)
 Frame = +3

Query: 510 ETEECMPLTVVLAHKLNPKIAELRRNG 590
           ET E MPL+ VLA K+  ++ E+R+NG
Sbjct: 133 ETPELMPLSHVLATKIGARLTEVRKNG 159


>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =   99 bits (238), Expect = 1e-21
 Identities = 47/86 (54%), Positives = 63/86 (73%), Gaps = 2/86 (2%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGAGDQGLMFGYAT 507
           GVH +  +  EE+GAGDQG MFGYAT
Sbjct: 106 GVHGHFTKCPEEIGAGDQGHMFGYAT 131



 Score = 81.0 bits (191), Expect = 6e-16
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47



 Score = 33.9 bits (74), Expect = 0.088
 Identities = 15/27 (55%), Positives = 20/27 (74%)
 Frame = +3

Query: 510 ETEECMPLTVVLAHKLNPKIAELRRNG 590
           ET E MPL+ VLA KL  ++ E+R+NG
Sbjct: 133 ETPELMPLSHVLATKLGARLTEVRKNG 159


>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =   99 bits (238), Expect = 1e-21
 Identities = 47/86 (54%), Positives = 63/86 (73%), Gaps = 2/86 (2%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGAGDQGLMFGYAT 507
           GVH +  +  EE+GAGDQG MFGYAT
Sbjct: 106 GVHGHFTKCPEEIGAGDQGHMFGYAT 131



 Score = 81.0 bits (191), Expect = 6e-16
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47



 Score = 33.9 bits (74), Expect = 0.088
 Identities = 15/27 (55%), Positives = 20/27 (74%)
 Frame = +3

Query: 510 ETEECMPLTVVLAHKLNPKIAELRRNG 590
           ET E MPL+ VLA KL  ++ E+R+NG
Sbjct: 133 ETPELMPLSHVLATKLGARLTEVRKNG 159


>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 3 (Methionine
           adenosyltransferase 3, AdoMet synthetase 3)
           [Lycopersicon esculentum] SWISS-PROT:P43282
          Length = 390

 Score = 98.3 bits (234), Expect = 4e-21
 Identities = 45/86 (52%), Positives = 63/86 (73%), Gaps = 2/86 (2%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+ A VDY+K+VR T + IG+  +  G D   C+V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGAGDQGLMFGYAT 507
           GVH +  +  E++GAGDQG MFGYAT
Sbjct: 106 GVHGHLTKKPEDIGAGDQGHMFGYAT 131



 Score = 79.8 bits (188), Expect = 1e-15
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTK 47



 Score = 34.3 bits (75), Expect = 0.067
 Identities = 16/26 (61%), Positives = 19/26 (73%)
 Frame = +3

Query: 510 ETEECMPLTVVLAHKLNPKIAELRRN 587
           ET E MPLT VLA KL  K+ E+R+N
Sbjct: 133 ETPELMPLTHVLATKLGAKLTEVRKN 158


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,178,346
Number of Sequences: 28952
Number of extensions: 341984
Number of successful extensions: 1001
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 961
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 996
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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