Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120500.Seq
(769 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09100.2 68416.m01070 mRNA capping enzyme family protein simi... 50 2e-06
At3g09100.1 68416.m01071 mRNA capping enzyme family protein simi... 50 2e-06
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 49 3e-06
At5g01290.1 68418.m00039 mRNA capping enzyme family protein simi... 49 3e-06
At5g39400.1 68418.m04773 pollen specific phosphatase, putative /... 42 6e-04
At5g23720.2 68418.m02781 dual specificity protein phosphatase fa... 31 0.64
At5g23720.1 68418.m02780 dual specificity protein phosphatase fa... 31 0.64
At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1 GI... 31 0.64
At3g19420.1 68416.m02463 expressed protein 31 1.1
At5g45170.1 68418.m05545 CbbY protein-related low similarity to ... 29 3.4
At3g23610.1 68416.m02970 dual specificity protein phosphatase (D... 29 4.5
At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing ... 28 7.9
At2g11370.1 68415.m01220 hypothetical protein 28 7.9
>At3g09100.2 68416.m01070 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF00782: Dual specificity phosphatase
catalytic domain
Length = 672
Score = 50.0 bits (114), Expect = 2e-06
Identities = 30/90 (33%), Positives = 46/90 (51%), Gaps = 3/90 (3%)
Frame = +1
Query: 250 GPLYKKIQVPGQ-TLPSESIVQEFIDTVEEFTE--KCPGMLVGVHCTHGINRTGYMVCRY 420
G + KI G+ +P V F++ V +F K + VHCTHG NRTG+M+ Y
Sbjct: 159 GIKHVKIACKGRDAVPDNVSVNAFVNEVNQFVLNLKHSKKYILVHCTHGHNRTGFMIVHY 218
Query: 421 LMHTLGIAPQEAINRFEKARGHKIERQNYV 510
LM + + +A+ F AR I + +Y+
Sbjct: 219 LMRSGPMNVTQALKIFSDARPPGIYKPDYI 248
Score = 27.9 bits (59), Expect = 7.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +2
Query: 185 IGAIIDLTNTSKYYDGVHFLRAG 253
+G +IDLTNT++YY + G
Sbjct: 137 LGLVIDLTNTTRYYSTTDLKKEG 159
>At3g09100.1 68416.m01071 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF00782: Dual specificity phosphatase
catalytic domain
Length = 471
Score = 50.0 bits (114), Expect = 2e-06
Identities = 30/90 (33%), Positives = 46/90 (51%), Gaps = 3/90 (3%)
Frame = +1
Query: 250 GPLYKKIQVPGQ-TLPSESIVQEFIDTVEEFTE--KCPGMLVGVHCTHGINRTGYMVCRY 420
G + KI G+ +P V F++ V +F K + VHCTHG NRTG+M+ Y
Sbjct: 159 GIKHVKIACKGRDAVPDNVSVNAFVNEVNQFVLNLKHSKKYILVHCTHGHNRTGFMIVHY 218
Query: 421 LMHTLGIAPQEAINRFEKARGHKIERQNYV 510
LM + + +A+ F AR I + +Y+
Sbjct: 219 LMRSGPMNVTQALKIFSDARPPGIYKPDYI 248
Score = 27.9 bits (59), Expect = 7.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +2
Query: 185 IGAIIDLTNTSKYYDGVHFLRAG 253
+G +IDLTNT++YY + G
Sbjct: 137 LGLVIDLTNTTRYYSTTDLKKEG 159
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 49.2 bits (112), Expect = 3e-06
Identities = 29/87 (33%), Positives = 46/87 (52%), Gaps = 3/87 (3%)
Frame = +1
Query: 259 YKKIQVPGQ-TLPSESIVQEFIDTVEEF-TEKCPGMLVGVHCTHGINRTGYMVCRYLMHT 432
Y KI+ G+ ++P V F+ V +F + VHCTHG NRTG+M+ YLM +
Sbjct: 121 YVKIRCSGRDSVPDNVSVNTFVHEVTQFENHNLSEKYLLVHCTHGHNRTGFMIVHYLMRS 180
Query: 433 LG-IAPQEAINRFEKARGHKIERQNYV 510
++ +A+ F AR I + +Y+
Sbjct: 181 RPMMSVTQALKIFSDARPPGIYKPDYI 207
Score = 28.7 bits (61), Expect = 4.5
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +2
Query: 185 IGAIIDLTNTSKYYDGVHFLRAGR 256
IG +IDLTNT++YY LR R
Sbjct: 95 IGLVIDLTNTTRYYHPNTELRQNR 118
>At5g01290.1 68418.m00039 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 607
Score = 49.2 bits (112), Expect = 3e-06
Identities = 31/91 (34%), Positives = 48/91 (52%), Gaps = 4/91 (4%)
Frame = +1
Query: 250 GPLYKKIQVPGQ-TLPSESIVQEFIDTVEEFT--EKCPGMLVGVHCTHGINRTGYMVCRY 420
G + KI G+ +P V F++ V +F +K V VHCTHG NRTG+M+ Y
Sbjct: 141 GIKHVKIACRGRDAVPDNVSVNTFVNEVLQFVLNQKHAKKYVLVHCTHGHNRTGFMIVHY 200
Query: 421 LMHTL-GIAPQEAINRFEKARGHKIERQNYV 510
LM ++ + +A+ F AR I + +Y+
Sbjct: 201 LMRSMPTMNVTQALKLFSDARPPGIYKPDYI 231
>At5g39400.1 68418.m04773 pollen specific phosphatase, putative /
phosphatase and tensin, putative (PTEN1) identical to
phosphatase and tensin homolog [Arabidopsis thaliana]
GI:21535746
Length = 412
Score = 41.5 bits (93), Expect = 6e-04
Identities = 21/64 (32%), Positives = 35/64 (54%)
Frame = +1
Query: 289 LPSESIVQEFIDTVEEFTEKCPGMLVGVHCTHGINRTGYMVCRYLMHTLGIAPQEAINRF 468
+PS ++Q F ++V + P + VHC G RTG MV YL++ G++ +EA+ +
Sbjct: 123 VPSLKMIQLFCESVHSWLSLDPKNIAVVHCMAGKGRTGLMVSAYLVYG-GMSAEEALEMY 181
Query: 469 EKAR 480
R
Sbjct: 182 ASRR 185
>At5g23720.2 68418.m02781 dual specificity protein phosphatase
family protein contains Pfam profile: PF00782 dual
specificity phosphatase, catalytic domain; contains TG
acceptor splice site at intron 9
Length = 845
Score = 31.5 bits (68), Expect = 0.64
Identities = 21/73 (28%), Positives = 33/73 (45%)
Frame = +1
Query: 298 ESIVQEFIDTVEEFTEKCPGMLVGVHCTHGINRTGYMVCRYLMHTLGIAPQEAINRFEKA 477
ESI QE +D ++ E G + VHC G +R+ +V YLM + EA ++ K
Sbjct: 684 ESIFQEALDFIKHGEET--GGKILVHCFEGRSRSATVVLAYLMLQKKLTLLEAWSKLRKV 741
Query: 478 RGHKIERQNYVKI 516
+ +I
Sbjct: 742 HRRAQPNDGFARI 754
>At5g23720.1 68418.m02780 dual specificity protein phosphatase
family protein contains Pfam profile: PF00782 dual
specificity phosphatase, catalytic domain; contains TG
acceptor splice site at intron 9
Length = 929
Score = 31.5 bits (68), Expect = 0.64
Identities = 21/73 (28%), Positives = 33/73 (45%)
Frame = +1
Query: 298 ESIVQEFIDTVEEFTEKCPGMLVGVHCTHGINRTGYMVCRYLMHTLGIAPQEAINRFEKA 477
ESI QE +D ++ E G + VHC G +R+ +V YLM + EA ++ K
Sbjct: 768 ESIFQEALDFIKHGEET--GGKILVHCFEGRSRSATVVLAYLMLQKKLTLLEAWSKLRKV 825
Query: 478 RGHKIERQNYVKI 516
+ +I
Sbjct: 826 HRRAQPNDGFARI 838
>At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1
GI:5566292 from [Drosophila melanogaster]; contains
prosite evidence: PS00383: Tyrosine specific protein
phosphatases active site
Length = 632
Score = 31.5 bits (68), Expect = 0.64
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +1
Query: 292 PSESIVQEFIDTVEEFTEKCPGMLVGVHCTHGINRTGYMVCRYLMHTLGIAP--QEAINR 465
P ++ F + + ++ +V VHC G+ RTG M+C L++ L P +EAI+
Sbjct: 279 PPIQLIPSFCQSAYTWLKEDIQNVVVVHCKAGMARTGLMICCLLLY-LKFFPTAEEAIDY 337
Query: 466 FEKAR 480
+ + R
Sbjct: 338 YNQKR 342
>At3g19420.1 68416.m02463 expressed protein
Length = 611
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = +1
Query: 292 PSESIVQEFIDTVEEFTEKCPGMLVGVHCTHGINRTGYMVCRYLMH 429
P +V F + + ++ +V VHC G+ RTG M+C L++
Sbjct: 235 PPIHLVTSFCQSAYSWLKEDIENVVVVHCKAGMARTGLMICSLLLY 280
>At5g45170.1 68418.m05545 CbbY protein-related low similarity to
SP|P40119 CbbY protein, chromosomal {Alcaligenes
eutrophus}
Length = 372
Score = 29.1 bits (62), Expect = 3.4
Identities = 15/35 (42%), Positives = 20/35 (57%)
Frame = +1
Query: 283 QTLPSESIVQEFIDTVEEFTEKCPGMLVGVHCTHG 387
++LP S VQEFID + EK P +V +C G
Sbjct: 174 KSLPLRSGVQEFIDNA--YAEKVPVAIVTAYCKSG 206
>At3g23610.1 68416.m02970 dual specificity protein phosphatase
(DsPTP1) identical to DsPTP1 protein GI:4150963 from
[Arabidopsis thaliana]; contains Pfam profile: PF00782
dual specificity phosphatase, catalytic domain
Length = 198
Score = 28.7 bits (61), Expect = 4.5
Identities = 22/76 (28%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +1
Query: 256 LYKKIQVPGQTLPS-ESIVQEFIDTVEEFTEKCPGMLVGVHCTHGINRTGYMVCRYLMHT 432
+YK ++V + + E E +D ++E K G V VHC G +R+ +V YLM
Sbjct: 96 VYKVVRVVDKEDTNLEMYFDECVDFIDE--AKRQGGSVLVHCFVGKSRSVTIVVAYLMKK 153
Query: 433 LGIAPQEAINRFEKAR 480
G+ +A+ + R
Sbjct: 154 HGMTLAQALQHVKSKR 169
>At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing
protein similar to SP|P34909 General negative regulator
of transcription subunit 4 {Saccharomyces cerevisiae};
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 989
Score = 27.9 bits (59), Expect = 7.9
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +2
Query: 128 VTSEEDVWTTEQIVKQNPSIGAIIDLTNTSKYYDG 232
V S+ED +T ++I+ + + I TNT +Y G
Sbjct: 218 VGSQEDSFTKDEIISAHTRVQQITGATNTMQYRSG 252
>At2g11370.1 68415.m01220 hypothetical protein
Length = 130
Score = 27.9 bits (59), Expect = 7.9
Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = +2
Query: 125 YVTSEEDVWTTEQ---IVKQNPSIGAIIDLTNTSKYYDGVHFLRAG 253
++ E V TT ++ NPS+G +D T + Y+D + L+ G
Sbjct: 50 FIKDVEAVLTTPDWANLLVLNPSLGTWVDRTTVTLYWDNMRALKRG 95
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,532,755
Number of Sequences: 28952
Number of extensions: 339461
Number of successful extensions: 940
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 940
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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