BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120488.Seq (823 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 2.4 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 27 2.4 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.4 SPBC21H7.04 |||ATP-dependent RNA helicase Dbp7 |Schizosaccharomy... 27 3.2 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.6 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 9.8 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 9.8 >SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 728 Score = 27.5 bits (58), Expect = 2.4 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +2 Query: 311 LITKSGVIQLIMKSKLPYAIELQEWLL 391 L+ K GV+ L+ K KLP+++ ++ ++ Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 27.5 bits (58), Expect = 2.4 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -3 Query: 587 SRFSVASSHNHFVGKQNERSVASAKSGWPP 498 S FS + SHNH Q E+S +++K P Sbjct: 537 SPFSKSKSHNHHPSSQVEKSTSNSKGSMLP 566 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 27.5 bits (58), Expect = 2.4 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = +1 Query: 196 STSTASTNRRLSMPTKSSTISR*RGKAGRPAVFAPTHSAHYQI 324 S+S+ S++R S T T S + + RP VF T +HY I Sbjct: 163 SSSSKSSSRSSSRTTSHRTTSH-KSSSYRPTVFPYTTISHYNI 204 >SPBC21H7.04 |||ATP-dependent RNA helicase Dbp7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 709 Score = 27.1 bits (57), Expect = 3.2 Identities = 14/49 (28%), Positives = 29/49 (59%), Gaps = 1/49 (2%) Frame = +2 Query: 461 VIAKIDDLTQKL-TVANQIWRKQPIAHFVCQRNDCGSTRR*NGSARLRK 604 ++A +L Q++ VAN++ P++H++ N G ++ + AR+RK Sbjct: 221 IMAPTRELCQQIYNVANKL-NNNPLSHWIVSCNVIGGEKKKSEKARIRK 268 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 26.2 bits (55), Expect = 5.6 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = +1 Query: 175 IVNGQYVSTSTASTNRRLSMPTKSSTIS 258 I G Y STS +ST+ + P+ SST S Sbjct: 129 ISGGIYSSTSASSTSSSTATPSSSSTTS 156 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 9.8 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 114 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 239 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 9.8 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +3 Query: 540 LFANEMIVARRDAETARQDCE 602 +FANE+++ + DA++ +QD E Sbjct: 556 IFANEILIEQADAQSFKQDEE 576 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,438,806 Number of Sequences: 5004 Number of extensions: 72903 Number of successful extensions: 221 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 221 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 402440190 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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