BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120479.Seq (830 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 54 2e-08 SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 44 3e-05 SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 37 0.004 SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 34 0.028 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 33 0.050 SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 32 0.087 SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 29 0.81 SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 27 2.5 SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 27 3.3 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 27 4.3 SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 5.7 SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 26 7.5 SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo... 26 7.5 >SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schizosaccharomyces pombe|chr 1|||Manual Length = 307 Score = 54.0 bits (124), Expect = 2e-08 Identities = 26/87 (29%), Positives = 48/87 (55%) Frame = +2 Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424 K +L+G+ +C+LD+ + + D+ QF IG RA ++ LNP+V++ + T Sbjct: 48 KNLVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVEIDTDT 107 Query: 425 KGVDELPDSFFTEFDVVCATGLKQEQF 505 + E+ + ++F +V AT L E+F Sbjct: 108 SLISEIDEGKISKFSMVIATQLDYEEF 134 Score = 49.2 bits (112), Expect = 7e-07 Identities = 24/51 (47%), Positives = 34/51 (66%) Frame = +3 Query: 99 VGNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251 +GN+ + E YDRQIRLWG ++Q+ L+ ++VL+I S L EIAKN Sbjct: 1 MGNHNINAEEIAL--YDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKN 49 Score = 27.9 bits (59), Expect = 1.9 Identities = 9/26 (34%), Positives = 15/26 (57%) Frame = +3 Query: 534 NKKFICGDVWGTYGYMFSDLVDHEYS 611 N F +G YG+ F DL++H ++ Sbjct: 145 NASFYATSCFGLYGFAFCDLINHNFA 170 >SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme |Schizosaccharomyces pombe|chr 2|||Manual Length = 1012 Score = 44.0 bits (99), Expect = 3e-05 Identities = 30/80 (37%), Positives = 39/80 (48%) Frame = +2 Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424 K L GVKSV L D + + DL SQ+ D IGV RA+ ++ + LN V V+ Sbjct: 54 KNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGVPRAKVTVSKLAELNQYVPVSV-- 111 Query: 425 KGVDELPDSFFTEFDVVCAT 484 VDEL + F V T Sbjct: 112 --VDELSTEYLKNFKCVVVT 129 Score = 40.3 bits (90), Expect = 3e-04 Identities = 18/36 (50%), Positives = 25/36 (69%) Frame = +3 Query: 144 YDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251 Y RQ+ + G ++ K++ + VLIIG GLG EIAKN Sbjct: 20 YSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKN 55 >SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 36.7 bits (81), Expect = 0.004 Identities = 14/43 (32%), Positives = 26/43 (60%) Frame = +3 Query: 123 SEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251 + A+ ++YDRQ+RLW + Q + + V ++ + +G E KN Sbjct: 3 TSAKMQKYDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKN 45 >SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 33.9 bits (74), Expect = 0.028 Identities = 20/45 (44%), Positives = 27/45 (60%), Gaps = 2/45 (4%) Frame = +3 Query: 105 NNEVELSEAEAEQYDRQIRLW--GLDSQKRLRAAKVLIIGLSGLG 233 +N +ELS E +Y RQ+ L GL Q L+ + VL+IG GLG Sbjct: 11 SNGLELSLDEYSRYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLG 55 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 33.1 bits (72), Expect = 0.050 Identities = 17/38 (44%), Positives = 27/38 (71%), Gaps = 1/38 (2%) Frame = +3 Query: 438 SYLTASSRNLTSYVQQVSNKSNSKNQQCL-SRQNKKFI 548 S+ T+S R+L S ++ +S K+N K++QCL S N +FI Sbjct: 765 SHSTSSKRDLLSGLESLSLKTNPKSEQCLESLLNSQFI 802 >SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 32.3 bits (70), Expect = 0.087 Identities = 20/79 (25%), Positives = 40/79 (50%), Gaps = 2/79 (2%) Frame = +2 Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424 K +++GVK V ++D + + +L QFL + +A + + A NP V + ++ Sbjct: 42 KNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHVKQPKAIVAAKTASSFNPNVKLEAYH 101 Query: 425 KGV--DELPDSFFTEFDVV 475 + D ++F +FD+V Sbjct: 102 ANIKEDRFNVAWFRQFDLV 120 Score = 30.3 bits (65), Expect = 0.35 Identities = 10/27 (37%), Positives = 20/27 (74%) Frame = +3 Query: 171 LDSQKRLRAAKVLIIGLSGLGAEIAKN 251 +++ + ++AKVL++G G+G E+ KN Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKN 43 >SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 29.1 bits (62), Expect = 0.81 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +3 Query: 138 EQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGA 236 EQ R +G D +RLR + V+++G G+G+ Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGS 139 >SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 27.5 bits (58), Expect = 2.5 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +3 Query: 174 DSQKRLRAAKVLIIGLSGLGAEIAKN 251 ++ K ++K+LIIG GLG EI K+ Sbjct: 37 ETLKSAFSSKILIIGAGGLGCEILKD 62 >SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor Sec74|Schizosaccharomyces pombe|chr 1|||Manual Length = 928 Score = 27.1 bits (57), Expect = 3.3 Identities = 36/113 (31%), Positives = 49/113 (43%), Gaps = 4/113 (3%) Frame = +2 Query: 137 RTVRPTNSPVGLRLAEETTCSESI--NHRLVRTRR*NCKKRILTGVKSVCLLDNEKLKQI 310 R+V P+ S +G A E T ES NH RR N R+ V+SV N KLK + Sbjct: 823 RSVFPS-SVMGKENASEDTILESNFRNHSNPSVRRSNL--RVGKTVESV---QNTKLKSV 876 Query: 311 DLYSQFLCPPDKIGVNR--AEGSLERARGLNPMVDVTSHTKGVDELPDSFFTE 463 + S + +G N S + + ++ V H V ELPD F E Sbjct: 877 ENDSG-KAETETVGKNPEVVRKSPAKLPNIANIMTVNGHRYSVVELPDDFLRE 928 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 26.6 bits (56), Expect = 4.3 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = +2 Query: 263 GVKSVCLLDNEKLKQIDLY 319 G SVCLL E LKQ +LY Sbjct: 89 GTTSVCLLVGELLKQAELY 107 >SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 26.2 bits (55), Expect = 5.7 Identities = 17/45 (37%), Positives = 25/45 (55%) Frame = +2 Query: 680 SFYNSKTSSYICPASECFIS*LEFTRDASRLRRGDCGYFAMKLLL 814 S +NS T +Y PAS+ +EF D +++R G +MK LL Sbjct: 294 SDFNSGTFTYATPASQT----IEFHSDYTKIRSGVYEGISMKHLL 334 >SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1019 Score = 25.8 bits (54), Expect = 7.5 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 561 WGTYGYMFSDLVDHEYSEEIVQHKATKRGPDDEEKNARETVS 686 WG G D D EE+ +++A+ P DEE+ E S Sbjct: 929 WGFLGAPSDDEGDDSV-EEVSEYEASDADPSDEEEEESEEYS 969 >SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 25.8 bits (54), Expect = 7.5 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = +1 Query: 508 RINNACRDRTKSLSAATSGVRTVTCSL 588 R+NN R+++ L + SG + + CSL Sbjct: 703 RMNNRAREKSLDLLRSDSGTQVLLCSL 729 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,256,578 Number of Sequences: 5004 Number of extensions: 64441 Number of successful extensions: 211 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 200 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 211 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 408446760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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