SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120479.Seq
         (830 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz...    54   2e-08
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ...    44   3e-05
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ...    37   0.004
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb...    34   0.028
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo...    33   0.050
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz...    32   0.087
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ...    29   0.81 
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ...    27   2.5  
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto...    27   3.3  
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C...    27   4.3  
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    26   5.7  
SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy...    26   7.5  
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo...    26   7.5  

>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
           Rad31|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 307

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 26/87 (29%), Positives = 48/87 (55%)
 Frame = +2

Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424
           K  +L+G+  +C+LD+  + + D+  QF      IG  RA    ++   LNP+V++ + T
Sbjct: 48  KNLVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVEIDTDT 107

Query: 425 KGVDELPDSFFTEFDVVCATGLKQEQF 505
             + E+ +   ++F +V AT L  E+F
Sbjct: 108 SLISEIDEGKISKFSMVIATQLDYEEF 134



 Score = 49.2 bits (112), Expect = 7e-07
 Identities = 24/51 (47%), Positives = 34/51 (66%)
 Frame = +3

Query: 99  VGNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
           +GN+ +   E     YDRQIRLWG ++Q+ L+ ++VL+I  S L  EIAKN
Sbjct: 1   MGNHNINAEEIAL--YDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKN 49



 Score = 27.9 bits (59), Expect = 1.9
 Identities = 9/26 (34%), Positives = 15/26 (57%)
 Frame = +3

Query: 534 NKKFICGDVWGTYGYMFSDLVDHEYS 611
           N  F     +G YG+ F DL++H ++
Sbjct: 145 NASFYATSCFGLYGFAFCDLINHNFA 170


>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1012

 Score = 44.0 bits (99), Expect = 3e-05
 Identities = 30/80 (37%), Positives = 39/80 (48%)
 Frame = +2

Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424
           K   L GVKSV L D +  +  DL SQ+    D IGV RA+ ++ +   LN  V V+   
Sbjct: 54  KNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGVPRAKVTVSKLAELNQYVPVSV-- 111

Query: 425 KGVDELPDSFFTEFDVVCAT 484
             VDEL   +   F  V  T
Sbjct: 112 --VDELSTEYLKNFKCVVVT 129



 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 18/36 (50%), Positives = 25/36 (69%)
 Frame = +3

Query: 144 YDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
           Y RQ+ + G ++ K++  + VLIIG  GLG EIAKN
Sbjct: 20  YSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKN 55


>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 500

 Score = 36.7 bits (81), Expect = 0.004
 Identities = 14/43 (32%), Positives = 26/43 (60%)
 Frame = +3

Query: 123 SEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
           + A+ ++YDRQ+RLW  + Q  +  + V ++  + +G E  KN
Sbjct: 3   TSAKMQKYDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKN 45


>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 401

 Score = 33.9 bits (74), Expect = 0.028
 Identities = 20/45 (44%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
 Frame = +3

Query: 105 NNEVELSEAEAEQYDRQIRLW--GLDSQKRLRAAKVLIIGLSGLG 233
           +N +ELS  E  +Y RQ+ L   GL  Q  L+ + VL+IG  GLG
Sbjct: 11  SNGLELSLDEYSRYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLG 55


>SPAC17C9.03 |tif471||translation initiation factor eIF4G
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1403

 Score = 33.1 bits (72), Expect = 0.050
 Identities = 17/38 (44%), Positives = 27/38 (71%), Gaps = 1/38 (2%)
 Frame = +3

Query: 438 SYLTASSRNLTSYVQQVSNKSNSKNQQCL-SRQNKKFI 548
           S+ T+S R+L S ++ +S K+N K++QCL S  N +FI
Sbjct: 765 SHSTSSKRDLLSGLESLSLKTNPKSEQCLESLLNSQFI 802


>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
           Fub2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 628

 Score = 32.3 bits (70), Expect = 0.087
 Identities = 20/79 (25%), Positives = 40/79 (50%), Gaps = 2/79 (2%)
 Frame = +2

Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424
           K  +++GVK V ++D + +   +L  QFL     +   +A  + + A   NP V + ++ 
Sbjct: 42  KNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHVKQPKAIVAAKTASSFNPNVKLEAYH 101

Query: 425 KGV--DELPDSFFTEFDVV 475
             +  D    ++F +FD+V
Sbjct: 102 ANIKEDRFNVAWFRQFDLV 120



 Score = 30.3 bits (65), Expect = 0.35
 Identities = 10/27 (37%), Positives = 20/27 (74%)
 Frame = +3

Query: 171 LDSQKRLRAAKVLIIGLSGLGAEIAKN 251
           +++ +  ++AKVL++G  G+G E+ KN
Sbjct: 17  VEALRNFKSAKVLLVGAGGIGCELLKN 43


>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 485

 Score = 29.1 bits (62), Expect = 0.81
 Identities = 12/33 (36%), Positives = 20/33 (60%)
 Frame = +3

Query: 138 EQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGA 236
           EQ  R    +G D  +RLR + V+++G  G+G+
Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGS 139


>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 444

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +3

Query: 174 DSQKRLRAAKVLIIGLSGLGAEIAKN 251
           ++ K   ++K+LIIG  GLG EI K+
Sbjct: 37  ETLKSAFSSKILIIGAGGLGCEILKD 62


>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
            Sec74|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 928

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 36/113 (31%), Positives = 49/113 (43%), Gaps = 4/113 (3%)
 Frame = +2

Query: 137  RTVRPTNSPVGLRLAEETTCSESI--NHRLVRTRR*NCKKRILTGVKSVCLLDNEKLKQI 310
            R+V P+ S +G   A E T  ES   NH     RR N   R+   V+SV    N KLK +
Sbjct: 823  RSVFPS-SVMGKENASEDTILESNFRNHSNPSVRRSNL--RVGKTVESV---QNTKLKSV 876

Query: 311  DLYSQFLCPPDKIGVNR--AEGSLERARGLNPMVDVTSHTKGVDELPDSFFTE 463
            +  S      + +G N      S  +   +  ++ V  H   V ELPD F  E
Sbjct: 877  ENDSG-KAETETVGKNPEVVRKSPAKLPNIANIMTVNGHRYSVVELPDDFLRE 928


>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
           Cct6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 12/19 (63%), Positives = 13/19 (68%)
 Frame = +2

Query: 263 GVKSVCLLDNEKLKQIDLY 319
           G  SVCLL  E LKQ +LY
Sbjct: 89  GTTSVCLLVGELLKQAELY 107


>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 570

 Score = 26.2 bits (55), Expect = 5.7
 Identities = 17/45 (37%), Positives = 25/45 (55%)
 Frame = +2

Query: 680 SFYNSKTSSYICPASECFIS*LEFTRDASRLRRGDCGYFAMKLLL 814
           S +NS T +Y  PAS+     +EF  D +++R G     +MK LL
Sbjct: 294 SDFNSGTFTYATPASQT----IEFHSDYTKIRSGVYEGISMKHLL 334


>SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1019

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = +3

Query: 561  WGTYGYMFSDLVDHEYSEEIVQHKATKRGPDDEEKNARETVS 686
            WG  G    D  D    EE+ +++A+   P DEE+   E  S
Sbjct: 929  WGFLGAPSDDEGDDSV-EEVSEYEASDADPSDEEEEESEEYS 969


>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +1

Query: 508 RINNACRDRTKSLSAATSGVRTVTCSL 588
           R+NN  R+++  L  + SG + + CSL
Sbjct: 703 RMNNRAREKSLDLLRSDSGTQVLLCSL 729


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,256,578
Number of Sequences: 5004
Number of extensions: 64441
Number of successful extensions: 211
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 211
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -