Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120479.Seq
(830 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 53 2e-07
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 52 4e-07
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 52 4e-07
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 52 5e-07
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 50 2e-06
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 46 3e-05
At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 36 0.044
At5g37530.1 68418.m04520 thiF family protein similar to SP|P3013... 35 0.058
At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 35 0.058
At5g19690.1 68418.m02342 oligosaccharyl transferase STT3 subunit... 31 0.71
At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3... 29 5.0
At3g02990.1 68416.m00294 heat shock factor protein 2 (HSF2) / he... 29 5.0
At4g30860.1 68417.m04381 SET domain-containing protein low simil... 28 8.7
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 28 8.7
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 28 8.7
>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
identical to SUMO activating enzyme 1a [Arabidopsis
thaliana] GI:22652850; contains Pfam profile PF00899:
ThiF family
Length = 322
Score = 53.2 bits (122), Expect = 2e-07
Identities = 23/45 (51%), Positives = 31/45 (68%)
Frame = +3
Query: 117 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
EL+E E YDRQIR+WG ++Q+RL A +L+ G+ G AE KN
Sbjct: 5 ELTEQETALYDRQIRVWGANAQRRLTKAHILVSGIKGTVAEFCKN 49
Score = 48.4 bits (110), Expect = 6e-06
Identities = 30/81 (37%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 242 CKKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDV 412
CK +L GV SV L+D+ L + FL PPD+ G AE + + NPMV V
Sbjct: 47 CKNIVLAGVGSVTLMDDRLANMEALNANFLIPPDENVYSGKTVAEICSDSLKDFNPMVRV 106
Query: 413 TSHTKGVDELPDSFFTEFDVV 475
+ + L FF +FDVV
Sbjct: 107 SVEKGDLSMLGTDFFEQFDVV 127
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 52.4 bits (120), Expect = 4e-07
Identities = 44/175 (25%), Positives = 79/175 (45%), Gaps = 8/175 (4%)
Frame = +3
Query: 117 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNXXXXXXXXXXXXTMR 296
EL+E E YDRQIR+WG +Q+RL + VL+ G+ G AE KN R
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDR 64
Query: 297 N*NKSICTPNFCVLLTRSA*IEQRV------RWKELEA*IRWSMSQVILRA-*MSYLTAS 455
+ NF +L +A + + V K+ + S+ + L + +
Sbjct: 65 LVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKF 124
Query: 456 SRNLTSYVQQVSNKSNSKNQQCLS-RQNKKFICGDVWGTYGYMFSDLVDHEYSEE 617
+ Y + + K + N++C + ++ F D G+ G +F DL +++Y+++
Sbjct: 125 DVVVIGYSSRATKK--AVNEKCRNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTKK 177
Score = 45.2 bits (102), Expect = 5e-05
Identities = 29/81 (35%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 242 CKKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDV 412
CK +L GV SV LLD+ + + FL PD+ +G AE + + NPMV V
Sbjct: 47 CKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHV 106
Query: 413 TSHTKGVDELPDSFFTEFDVV 475
+ + L FF +FDVV
Sbjct: 107 SIEKGDLSTLGVDFFEKFDVV 127
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 52.4 bits (120), Expect = 4e-07
Identities = 44/175 (25%), Positives = 79/175 (45%), Gaps = 8/175 (4%)
Frame = +3
Query: 117 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNXXXXXXXXXXXXTMR 296
EL+E E YDRQIR+WG +Q+RL + VL+ G+ G AE KN R
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDR 64
Query: 297 N*NKSICTPNFCVLLTRSA*IEQRV------RWKELEA*IRWSMSQVILRA-*MSYLTAS 455
+ NF +L +A + + V K+ + S+ + L + +
Sbjct: 65 LVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKF 124
Query: 456 SRNLTSYVQQVSNKSNSKNQQCLS-RQNKKFICGDVWGTYGYMFSDLVDHEYSEE 617
+ Y + + K + N++C + ++ F D G+ G +F DL +++Y+++
Sbjct: 125 DVVVIGYSSRATKK--AVNEKCRNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTKK 177
Score = 45.2 bits (102), Expect = 5e-05
Identities = 29/81 (35%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 242 CKKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDV 412
CK +L GV SV LLD+ + + FL PD+ +G AE + + NPMV V
Sbjct: 47 CKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHV 106
Query: 413 TSHTKGVDELPDSFFTEFDVV 475
+ + L FF +FDVV
Sbjct: 107 SIEKGDLSTLGVDFFEKFDVV 127
>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 318
Score = 52.0 bits (119), Expect = 5e-07
Identities = 29/85 (34%), Positives = 41/85 (48%)
Frame = +3
Query: 117 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNXXXXXXXXXXXXTMR 296
EL+E E YDRQIR+WG +Q+RL + VL+ G+ G AE KN R
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDR 64
Query: 297 N*NKSICTPNFCVLLTRSA*IEQRV 371
+ NF +L +A + + V
Sbjct: 65 LVTTEVFNANFLILPDENAYVGKTV 89
Score = 45.2 bits (102), Expect = 5e-05
Identities = 29/81 (35%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 242 CKKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDV 412
CK +L GV SV LLD+ + + FL PD+ +G AE + + NPMV V
Sbjct: 47 CKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHV 106
Query: 413 TSHTKGVDELPDSFFTEFDVV 475
+ + L FF +FDVV
Sbjct: 107 SIEKGDLSTLGVDFFEKFDVV 127
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 50.0 bits (114), Expect = 2e-06
Identities = 27/65 (41%), Positives = 40/65 (61%)
Frame = +2
Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424
K IL GVKSV L D ++ DL S F+ D +G NRA+ S+++ + LN V V+S T
Sbjct: 112 KNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLT 171
Query: 425 KGVDE 439
K +++
Sbjct: 172 KSLNK 176
Score = 45.2 bits (102), Expect = 5e-05
Identities = 20/42 (47%), Positives = 31/42 (73%)
Frame = +3
Query: 126 EAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
E + + + RQ+ ++G ++ +RL A+ VLI G+ GLGAEIAKN
Sbjct: 72 EIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKN 113
Score = 37.5 bits (83), Expect = 0.011
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = +3
Query: 141 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
+YD QI ++G QK+L AKV +G LG E KN
Sbjct: 474 RYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKN 510
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 46.0 bits (104), Expect = 3e-05
Identities = 20/50 (40%), Positives = 33/50 (66%)
Frame = +3
Query: 102 GNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
GN+ E + + + RQ+ ++G ++ ++L A+ VLI G+ GLG EIAKN
Sbjct: 61 GNDNSNNQEIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKN 110
Score = 42.7 bits (96), Expect = 3e-04
Identities = 31/89 (34%), Positives = 45/89 (50%)
Frame = +2
Query: 245 KKRILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHT 424
K IL GVKSV L D ++ DL S F+ + IG NRA S+ + + LN V V++ T
Sbjct: 109 KNIILAGVKSVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLT 168
Query: 425 KGVDELPDSFFTEFDVVCATGLKQEQFEE 511
+L ++F VV + E+ E
Sbjct: 169 ---GKLTKEQLSDFQVVVFVDISFEKATE 194
Score = 37.9 bits (84), Expect = 0.008
Identities = 16/37 (43%), Positives = 23/37 (62%)
Frame = +3
Query: 141 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
+YD QI ++G QK+L A+V ++G LG E KN
Sbjct: 471 RYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKN 507
>At2g32410.1 68415.m03960 auxin-resistance protein, putative strong
similarity to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family
Length = 523
Score = 35.5 bits (78), Expect = 0.044
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 141 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
+YDRQ+R+WG Q L A + ++ G+E KN
Sbjct: 7 KYDRQLRIWGELGQSALETASICLLNCGPTGSEALKN 43
>At5g37530.1 68418.m04520 thiF family protein similar to SP|P30138
Adenylyltransferase thiF (EC 2.7.7.-) {Escherichia
coli}; contains Pfam profile PF00899: ThiF family
Length = 457
Score = 35.1 bits (77), Expect = 0.058
Identities = 15/40 (37%), Positives = 26/40 (65%)
Frame = +3
Query: 126 EAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIA 245
E +E R I+ +GL+SQ ++ + V++IGL G+G+ A
Sbjct: 70 EIVSEHLTRNIQFFGLESQHKVTGSYVVVIGLGGVGSHAA 109
>At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 540
Score = 35.1 bits (77), Expect = 0.058
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 141 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKN 251
+YDRQ+R+WG Q L A + ++ G+E KN
Sbjct: 23 KYDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKN 59
>At5g19690.1 68418.m02342 oligosaccharyl transferase STT3 subunit
family protein similar to SP|P39007 Oligosaccharyl
transferase STT3 subunit {Saccharomyces cerevisiae};
contains Pfam profile PF02516: Oligosaccharyl
transferase STT3 subunit
Length = 779
Score = 31.5 bits (68), Expect = 0.71
Identities = 14/44 (31%), Positives = 30/44 (68%), Gaps = 1/44 (2%)
Frame = +3
Query: 612 EEIVQHKATKRGPDDEEKNA-RETVSITVKRRAIYVPLQNALSA 740
EEIV+ +++K+G E + A + +V +K++A+ +PL+ ++ A
Sbjct: 478 EEIVKERSSKKGKKKEREPADKPSVKAKIKKKALVLPLEASIVA 521
>At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3 /
molybdopterin synthase sulphurylase (CNX5) identical to
SP|Q9ZNW0 Molybdenum cofactor synthesis protein 3
(Molybdopterin synthase sulfurylase) (MPT synthase
sulfurylase) {Arabidopsis thaliana}; contains Pfam
profiles PF00899: ThiF family, PF00581: Rhodanese-like
domain, PF05237: MoeZ/MoeB domain
Length = 464
Score = 28.7 bits (61), Expect = 5.0
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = +3
Query: 120 LSEAEAEQYDRQIRL--WGLDSQKRLRAAKVLIIGLSGLGA 236
LS + +Y RQ+ L + ++ Q L + VL+IG GLG+
Sbjct: 64 LSPDQIYRYSRQLLLPSFAVEGQSNLLKSSVLVIGAGGLGS 104
>At3g02990.1 68416.m00294 heat shock factor protein 2 (HSF2) / heat
shock transcription factor 2 (HSTF2) identical to heat
shock transcription factor 2 (HSF2) SP:Q96320 from
[Arabidopsis thaliana]; contains Pfam profile: PF00447
HSF-type DNA-binding domain
Length = 468
Score = 28.7 bits (61), Expect = 5.0
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +3
Query: 441 YLTASSRNLTSYVQQVSNKSNSKNQQCLSRQNKKFICGDV 560
Y ++ + + +QQ+ SN+ + + LS N F+ GDV
Sbjct: 251 YQSSMNDATNTMLQQIQQMSNAPSHESLSSNNGSFLLGDV 290
>At4g30860.1 68417.m04381 SET domain-containing protein low
similarity to IL-5 promoter REII-region-binding protein
[Homo sapiens] GI:12642795; contains Pfam profile
PF00856: SET domain
Length = 497
Score = 27.9 bits (59), Expect = 8.7
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 668 CPRNSFYNSKTSSYICPASECFI 736
C + S SK+S + CP ECF+
Sbjct: 149 CAKESLGFSKSSKFKCPQHECFV 171
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 27.9 bits (59), Expect = 8.7
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +3
Query: 180 QKRLRAAKVLIIGLSGLGAEIAK 248
Q ++ AKVL++G G+G E+ K
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLK 29
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 27.9 bits (59), Expect = 8.7
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +3
Query: 180 QKRLRAAKVLIIGLSGLGAEIAK 248
Q ++ AKVL++G G+G E+ K
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLK 29
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,823,478
Number of Sequences: 28952
Number of extensions: 332679
Number of successful extensions: 1010
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1006
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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