BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120448.Seq (746 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 31 0.17 SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 31 0.17 SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo... 29 0.70 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 3.8 SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 26 5.0 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 6.6 SPAC1F7.07c |fip1||iron permease Fip1|Schizosaccharomyces pombe|... 26 6.6 SPBC342.06c |rtt109|kat11|RTT109 family histone lysine acetyltra... 25 8.7 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 25 8.7 SPCC1183.09c |pmp31|mug75|plasma membrane proteolipid Pmp31|Schi... 25 8.7 SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyce... 25 8.7 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 31.1 bits (67), Expect = 0.17 Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = -3 Query: 663 IAVKLFPIKLAELPRFSCTPECSW-ICAIRAPLVRTAAPCWGRSSTQSCCRLR 508 + L+ + ++ RFS SW + +AP V + + C S ++SCC LR Sbjct: 1758 LVANLWDVTDKDIDRFSLKMLESWGLFENKAPFVNSTSICTAVSESRSCCHLR 1810 >SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 744 Score = 31.1 bits (67), Expect = 0.17 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Frame = -1 Query: 464 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 285 P+ + L L+S A R T L + A G + + PR++L V EP+ + S Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442 Query: 284 SID--SGFS 264 S++ SGFS Sbjct: 443 SLNNFSGFS 451 >SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 385 Score = 29.1 bits (62), Expect = 0.70 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%) Frame = -3 Query: 423 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 310 K+CG A HGH R R+W F + Y +K ++ F Sbjct: 39 KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 26.6 bits (56), Expect = 3.8 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +1 Query: 283 DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 408 D I + G NK S+ + T+ + SPN+ +T+SN T+ Sbjct: 891 DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932 >SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 26.2 bits (55), Expect = 5.0 Identities = 14/52 (26%), Positives = 26/52 (50%) Frame = +1 Query: 286 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 441 D +KE + K +S+F ++Y PNA+ N T ++D++E + Sbjct: 64 DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = -1 Query: 362 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSG 270 LTP +P+ ++L+P L+ L S+I SG Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSG 703 >SPAC1F7.07c |fip1||iron permease Fip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 397 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/42 (28%), Positives = 19/42 (45%) Frame = -3 Query: 309 VGALFDYVVYR*RFFGNILWLNLLSPCAMRKLATRALLSFTF 184 VG L Y +YR N+ W + S C + ++ + TF Sbjct: 188 VGCLIGYFIYRGGNVMNLQWFLIASTCILYLISAGLMSKATF 229 >SPBC342.06c |rtt109|kat11|RTT109 family histone lysine acetyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 369 Score = 25.4 bits (53), Expect = 8.7 Identities = 8/18 (44%), Positives = 13/18 (72%) Frame = -3 Query: 630 ELPRFSCTPECSWICAIR 577 ELPRF P+C ++C ++ Sbjct: 212 ELPRFPDDPKCRYLCELQ 229 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 25.4 bits (53), Expect = 8.7 Identities = 14/34 (41%), Positives = 21/34 (61%) Frame = -1 Query: 410 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 309 P V++ V AF +TP YTVP+ +++PTL Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415 >SPCC1183.09c |pmp31|mug75|plasma membrane proteolipid Pmp31|Schizosaccharomyces pombe|chr 3|||Manual Length = 105 Score = 25.4 bits (53), Expect = 8.7 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = -2 Query: 277 IAVFRQYTLAQFIIAVCDAQIGHPGVI 197 + + R + A F+I +C +G PG+I Sbjct: 23 VGIRRGFCTADFLINICLCALGIPGII 49 >SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyces pombe|chr 3|||Manual Length = 210 Score = 25.4 bits (53), Expect = 8.7 Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 2/49 (4%) Frame = +2 Query: 113 SLTEASPSLGQSSESVESDENKRLNVKLN--NARVANLRIAHGDNKLSQ 253 SLT+A SL + S+SVE + +L+ +L+ ++AN R G L Q Sbjct: 16 SLTDAIDSLDKRSDSVEV-KIAKLDAQLSVFQQKIANTRPGPGQTALKQ 63 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,038,876 Number of Sequences: 5004 Number of extensions: 62580 Number of successful extensions: 170 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 170 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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