BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120440.Seq (697 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 1.9 SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 7.8 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/55 (27%), Positives = 28/55 (50%) Frame = +1 Query: 484 VITYFGRY*RKR*NLRVALLD*EL*IEQKKMLKVLLNTWMYSHGRRYYCKIMYVS 648 VITY + ++ + LLD + E + + +LL+ W+ H + Y + +VS Sbjct: 338 VITYMTKEQEEKDSTNSFLLDKDTGAEMELVSSMLLSEWLAEHYKDYGANLEFVS 392 >SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 25.4 bits (53), Expect = 7.8 Identities = 10/33 (30%), Positives = 18/33 (54%) Frame = -2 Query: 195 EQTVATFTNRNLLTIYDIMHHKTTSQINDVLST 97 E + F +RNL++ YD+ +N +L+T Sbjct: 204 EDVLLHFWDRNLMSTYDLKIQDINDSVNPLLNT 236 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,115,923 Number of Sequences: 5004 Number of extensions: 34559 Number of successful extensions: 70 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 67 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 70 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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