SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120433.Seq
         (767 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g37060.1 68417.m05248 patatin, putative similar to patatin-li...    33   0.28 
At5g10130.1 68418.m01173 pollen Ole e 1 allergen and extensin fa...    30   2.0  
At2g40630.1 68415.m05011 expressed protein                             29   2.6  
At3g58770.1 68416.m06550 expressed protein  ; expression support...    29   3.4  
At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family...    29   3.4  
At5g24150.1 68418.m02839 squalene monooxygenase 1,1 / squalene e...    29   4.5  
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain...    29   4.5  
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain...    29   4.5  
At5g02730.1 68418.m00214 allergen V5/Tpx-1-related family protei...    29   4.5  

>At4g37060.1 68417.m05248 patatin, putative similar to patatin-like
           latex allergen [Hevea brasiliensis][PMID:10589016];
           contains patatin domain PF01734
          Length = 414

 Score = 32.7 bits (71), Expect = 0.28
 Identities = 28/84 (33%), Positives = 38/84 (45%), Gaps = 4/84 (4%)
 Frame = +3

Query: 171 TFEHADQIQHHAPDSVAK--QGDPLYLT-PTTVLITKSGVIQLIMKSKLPYAIELQEWLL 341
           TFE +  I H+    V K  Q +  YL      L   +  + L  KS L   I+L E +L
Sbjct: 295 TFESSRDIVHYHSSVVFKALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKML 354

Query: 342 EEVIPQV-LCTGKYAPAVEMDTND 410
              + Q+ + TG Y PA E   ND
Sbjct: 355 TNRVMQMNIDTGTYEPAAENINND 378


>At5g10130.1 68418.m01173 pollen Ole e 1 allergen and extensin
           family protein contains similarity to pollen specific
           protein C13 precursor [Zea mays] SWISS-PROT:P33050
          Length = 164

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 29/91 (31%), Positives = 40/91 (43%), Gaps = 6/91 (6%)
 Frame = +3

Query: 24  KFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHADQIQH- 200
           +FG +T   +Y+ G    VR V KD  + LK      A+    DGKYK       Q Q  
Sbjct: 40  RFGFETIATQYIRGAR--VRIVCKDRVT-LKSELVGVAV-TGPDGKYKVAVRGDRQDQQC 95

Query: 201 -----HAPDSVAKQGDPLYLTPTTVLITKSG 278
                H+P S  ++ DP   T T +L   +G
Sbjct: 96  LAELVHSPLSRCQEADPGRSTATVILTRSNG 126


>At2g40630.1 68415.m05011 expressed protein
          Length = 535

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 17/56 (30%), Positives = 23/56 (41%)
 Frame = +3

Query: 537 SARLRKRAPRNGAAGQPHGGHCARRDSQTQQPAAVPQPGRVRRGHNEFAFLRRKND 704
           S R  +   RNG    P GG  A   +    P  VP P + +        L++KND
Sbjct: 206 SMRRWREMKRNGFLSGPLGGVAAPTSTVVTTPVEVPAPKQQKNKRRGGESLKKKND 261


>At3g58770.1 68416.m06550 expressed protein  ; expression supported
           by MPSS
          Length = 771

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = -1

Query: 758 SLLKMTSLDPIVSGCGPAIVFAAQKRKLVVPTS 660
           +LL+ TS DP    C P +  A+Q R   VP S
Sbjct: 580 ALLRDTSKDPFPLFCEPLVSLASQSRAYAVPDS 612


>At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family
           protein
          Length = 635

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 19/68 (27%), Positives = 32/68 (47%)
 Frame = -1

Query: 698 FAAQKRKLVVPTSHTARLWHSCGLLGLAITSCAMSAMRLASCAVSRRAFSQSCRAVSASR 519
           FA         +S T+  +HS  LLG A    ++S+    +C  + +AF Q  +A   S 
Sbjct: 177 FATSSSASATSSSSTSSPFHSSSLLGFAPAVTSVSSAPTPACGPT-QAFGQPTQAFGLSM 235

Query: 518 RATTFRWQ 495
             +T R++
Sbjct: 236 FGSTPRFE 243


>At5g24150.1 68418.m02839 squalene monooxygenase 1,1 / squalene
           epoxidase 1,1 (SQP1,1) identical to SP|O65404
          Length = 516

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 20/57 (35%), Positives = 26/57 (45%)
 Frame = -1

Query: 677 LVVPTSHTARLWHSCGLLGLAITSCAMSAMRLASCAVSRRAFSQSCRAVSASRRATT 507
           L+ P     R+WHS  L GLA+    M    L +  VS+  F  +  A S S  A T
Sbjct: 461 LLSPFPSPLRIWHSLRLFGLAM---KMLVPHLKAEGVSQMLFPVNAAAYSKSYMAAT 514


>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 870

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 17/47 (36%), Positives = 22/47 (46%)
 Frame = +3

Query: 3   QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 143
           Q   G F    DT   +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 822

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 17/47 (36%), Positives = 22/47 (46%)
 Frame = +3

Query: 3   QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 143
           Q   G F    DT   +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At5g02730.1 68418.m00214 allergen V5/Tpx-1-related family protein
           low similarity to SP|Q05968 Pathogenesis-related protein
           1 precursor {Hordeum vulgare}; contains Pfam profile
           PF00188: SCP-like extracellular protein
          Length = 205

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 12/38 (31%), Positives = 20/38 (52%)
 Frame = -1

Query: 551 SQSCRAVSASRRATTFRWQTK*AIGCFRQICVGHRQFL 438
           + +C++ +     T   W+T  A+GC R  C  +R FL
Sbjct: 139 ANTCKSGAMCGHYTQIVWRTTTAVGCARSKCDNNRGFL 176


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,393,416
Number of Sequences: 28952
Number of extensions: 418106
Number of successful extensions: 1180
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1180
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -