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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120427.Seq
         (752 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe...    29   0.94 
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos...    28   1.2  
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    26   5.0  
SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit...    26   6.6  
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar...    26   6.6  

>SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 652

 Score = 28.7 bits (61), Expect = 0.94
 Identities = 15/67 (22%), Positives = 33/67 (49%)
 Frame = +1

Query: 202 PKPRVSGHTRHSMQQLRRHLNSDMGVVMLVEEYFYCLSSCNFKQDAKLALQTLTNFESLS 381
           P  R S  T   + QL+++  S  G+++ +  YF  +   N+  +  +    ++ + S+S
Sbjct: 488 PDARFSYFTGSILVQLKKY--SQSGILVYIPSYFDFVRVRNYMDEEGINYSAISEYSSVS 545

Query: 382 DLMASYN 402
           D+  + N
Sbjct: 546 DMTRARN 552


>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 16/40 (40%), Positives = 22/40 (55%)
 Frame = -1

Query: 386 RSDSDSKFVSVCSASLASCLKLHDDRQ*KYSSTSMTTPIS 267
           +S SDS   S  S+S +  + L+DD    YSS +  T IS
Sbjct: 112 KSYSDSSTSSARSSSTSGSISLYDDYYPAYSSNAPNTAIS 151


>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 268

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 11/39 (28%), Positives = 19/39 (48%), Gaps = 2/39 (5%)
 Frame = +2

Query: 140 DKLFRCRLCYTHADWLWCDFHRNHAYRG--TRDIACNNY 250
           D+   C +C  H D ++  FH +  Y+   T+ +  N Y
Sbjct: 52  DQSLTCPICKRHVDCVFYGFHGSSLYKKWYTQSLGSNQY 90


>SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit
           Skp1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 161

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 7/15 (46%), Positives = 10/15 (66%)
 Frame = +2

Query: 188 WCDFHRNHAYRGTRD 232
           WC+ H+N  Y GT +
Sbjct: 57  WCEHHKNDLYSGTEE 71


>SPAPB1A10.07c |||sphingolipid biosynthesis
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 441

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 10/22 (45%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
 Frame = +1

Query: 271 MGVVMLVEEY-FYCLSSCNFKQ 333
           +G+V+ +  Y F+C SSC+F Q
Sbjct: 212 VGLVLTILTYVFFCASSCSFNQ 233


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,966,871
Number of Sequences: 5004
Number of extensions: 60729
Number of successful extensions: 161
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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