BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120427.Seq (752 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe... 29 0.94 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 28 1.2 SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 5.0 SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit... 26 6.6 SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 6.6 >SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 652 Score = 28.7 bits (61), Expect = 0.94 Identities = 15/67 (22%), Positives = 33/67 (49%) Frame = +1 Query: 202 PKPRVSGHTRHSMQQLRRHLNSDMGVVMLVEEYFYCLSSCNFKQDAKLALQTLTNFESLS 381 P R S T + QL+++ S G+++ + YF + N+ + + ++ + S+S Sbjct: 488 PDARFSYFTGSILVQLKKY--SQSGILVYIPSYFDFVRVRNYMDEEGINYSAISEYSSVS 545 Query: 382 DLMASYN 402 D+ + N Sbjct: 546 DMTRARN 552 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 28.3 bits (60), Expect = 1.2 Identities = 16/40 (40%), Positives = 22/40 (55%) Frame = -1 Query: 386 RSDSDSKFVSVCSASLASCLKLHDDRQ*KYSSTSMTTPIS 267 +S SDS S S+S + + L+DD YSS + T IS Sbjct: 112 KSYSDSSTSSARSSSTSGSISLYDDYYPAYSSNAPNTAIS 151 >SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 26.2 bits (55), Expect = 5.0 Identities = 11/39 (28%), Positives = 19/39 (48%), Gaps = 2/39 (5%) Frame = +2 Query: 140 DKLFRCRLCYTHADWLWCDFHRNHAYRG--TRDIACNNY 250 D+ C +C H D ++ FH + Y+ T+ + N Y Sbjct: 52 DQSLTCPICKRHVDCVFYGFHGSSLYKKWYTQSLGSNQY 90 >SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit Skp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 161 Score = 25.8 bits (54), Expect = 6.6 Identities = 7/15 (46%), Positives = 10/15 (66%) Frame = +2 Query: 188 WCDFHRNHAYRGTRD 232 WC+ H+N Y GT + Sbjct: 57 WCEHHKNDLYSGTEE 71 >SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 25.8 bits (54), Expect = 6.6 Identities = 10/22 (45%), Positives = 16/22 (72%), Gaps = 1/22 (4%) Frame = +1 Query: 271 MGVVMLVEEY-FYCLSSCNFKQ 333 +G+V+ + Y F+C SSC+F Q Sbjct: 212 VGLVLTILTYVFFCASSCSFNQ 233 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,966,871 Number of Sequences: 5004 Number of extensions: 60729 Number of successful extensions: 161 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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