BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120416X.Seq (536 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g25070.1 68418.m02971 expressed protein 42 3e-04 At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 31 0.49 At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 31 0.49 At4g23800.1 68417.m03422 high mobility group (HMG1/2) family pro... 31 0.49 At1g33820.1 68414.m04185 hypothetical protein 29 1.5 At1g15040.2 68414.m01798 glutamine amidotransferase-related 29 1.5 At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos... 29 2.0 At5g22040.1 68418.m02566 expressed protein 29 2.6 At1g15040.1 68414.m01797 glutamine amidotransferase-related 29 2.6 At5g18510.1 68418.m02185 hypothetical protein 28 4.5 At1g77960.1 68414.m09085 hypothetical protein 28 4.5 At1g24560.1 68414.m03090 expressed protein 28 4.5 At3g61380.1 68416.m06869 expressed protein 27 6.0 At3g26540.1 68416.m03313 pentatricopeptide (PPR) repeat-containi... 27 6.0 At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 tr... 27 6.0 At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 tr... 27 6.0 At1g10880.1 68414.m01250 expressed protein contains Pfam profile... 27 7.9 >At5g25070.1 68418.m02971 expressed protein Length = 736 Score = 41.9 bits (94), Expect = 3e-04 Identities = 22/82 (26%), Positives = 41/82 (50%) Frame = -2 Query: 250 QKTEELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMHKKD 71 +K E L + E + ++ K+K+I+ D Q+ V +N + TGF+E D+M++ Sbjct: 384 KKKEHLANELEELLALVKAKEKEIDENDSQIEAVEERINNVVTGFKELQTSMDKMLNDVQ 443 Query: 70 ELLQVKDTQVSNLIAKMIDLSD 5 L D + +L K D+ + Sbjct: 444 AGLTEVDKETEDLSRKKKDVDE 465 >At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing protein contains Pfam profile PF01465: GRIP domain; supporting cDNA gi|20303028|gb|AF499634.1| Length = 765 Score = 31.1 bits (67), Expect = 0.49 Identities = 19/86 (22%), Positives = 41/86 (47%), Gaps = 2/86 (2%) Frame = -2 Query: 295 CDIYRAHKRAASGGAQKTE-ELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTG 119 CD ++ A + E E+ K + + ++++ + + KD ++ ++NR+ + Sbjct: 371 CDALKSKLEIAESNYLQAEIEVAKMRSQLGSEMSMQTQILSTKDAELKGAREEINRLQSE 430 Query: 118 FQETMQRKDEMMHKKD-ELLQVKDTQ 44 F R ++ KKD EL KD++ Sbjct: 431 FSSYKIRAHALLQKKDMELAAAKDSE 456 >At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing protein contains Pfam profile PF01465: GRIP domain; supporting cDNA gi|20303028|gb|AF499634.1| Length = 788 Score = 31.1 bits (67), Expect = 0.49 Identities = 19/86 (22%), Positives = 41/86 (47%), Gaps = 2/86 (2%) Frame = -2 Query: 295 CDIYRAHKRAASGGAQKTE-ELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTG 119 CD ++ A + E E+ K + + ++++ + + KD ++ ++NR+ + Sbjct: 371 CDALKSKLEIAESNYLQAEIEVAKMRSQLGSEMSMQTQILSTKDAELKGAREEINRLQSE 430 Query: 118 FQETMQRKDEMMHKKD-ELLQVKDTQ 44 F R ++ KKD EL KD++ Sbjct: 431 FSSYKIRAHALLQKKDMELAAAKDSE 456 >At4g23800.1 68417.m03422 high mobility group (HMG1/2) family protein similar to HMG2B [Homo sapiens] GI:32335; contains Pfam profile PF00505: HMG (high mobility group) box Length = 456 Score = 31.1 bits (67), Expect = 0.49 Identities = 19/74 (25%), Positives = 39/74 (52%), Gaps = 6/74 (8%) Frame = -2 Query: 235 LFKKQEFIERI---IAIKDKQIEAKDLQVTRVMTDLNRMYTG---FQETMQRKDEMMHKK 74 L +K E +E +++K K ++ + + + T L +M +E ++ KDE++ KK Sbjct: 20 LKQKNELVETPPSPVSVKGKSAKSFEQDLMEMQTMLEKMKIEKDKTEELLKEKDEILRKK 79 Query: 73 DELLQVKDTQVSNL 32 +E L+ +D + L Sbjct: 80 EEELETRDAEQEKL 93 >At1g33820.1 68414.m04185 hypothetical protein Length = 182 Score = 29.5 bits (63), Expect = 1.5 Identities = 16/51 (31%), Positives = 27/51 (52%) Frame = -2 Query: 172 KDLQVTRVMTDLNRMYTGFQETMQRKDEMMHKKDELLQVKDTQVSNLIAKM 20 KD Q ++ + M T F+E + M + + LQ+KDTQ N++ +M Sbjct: 72 KDTQTMNMVKQM--METAFKENAMVHERDMCQLKDTLQLKDTQTINMMTQM 120 >At1g15040.2 68414.m01798 glutamine amidotransferase-related Length = 333 Score = 29.5 bits (63), Expect = 1.5 Identities = 13/48 (27%), Positives = 27/48 (56%) Frame = -2 Query: 223 QEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMH 80 QEF++ +IA + KQ+ A +++ R T L + ++ + R ++H Sbjct: 268 QEFVKAVIAFQKKQVNATQVEMKRKTTTLVKSFSQAEFLEVRDSFVLH 315 >At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative similar to SP:P24859 from [Kluyveromyces lactissimilar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 608 Score = 29.1 bits (62), Expect = 2.0 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = -2 Query: 160 VTRVMTDLNRMYTGFQETMQRKDEMMHKKDELLQVKDTQVSNLIAKMI 17 ++ V++ L + + RK EM H+K+ELL +V L A++I Sbjct: 526 ISSVLSRLGDLEKQIENLHSRKSEMPHEKEELLNAAVYRVDALEAELI 573 >At5g22040.1 68418.m02566 expressed protein Length = 284 Score = 28.7 bits (61), Expect = 2.6 Identities = 17/67 (25%), Positives = 36/67 (53%), Gaps = 1/67 (1%) Frame = -2 Query: 256 GAQKTEELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTGFQETMQR-KDEMMH 80 G Q E L +Q+F++ I + QIEA+D ++ R +N + ++E + + Sbjct: 120 GVQTFEGL--EQKFMDDITRLAKDQIEAEDAEIARHREKINTINARYEEQLATLRARHTG 177 Query: 79 KKDELLQ 59 K++E+++ Sbjct: 178 KREEIMR 184 >At1g15040.1 68414.m01797 glutamine amidotransferase-related Length = 395 Score = 28.7 bits (61), Expect = 2.6 Identities = 11/34 (32%), Positives = 22/34 (64%) Frame = -2 Query: 223 QEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYT 122 QEF++ +IA + KQ+ A +++ R T L + ++ Sbjct: 268 QEFVKAVIAFQKKQVNATQVEMKRKTTTLVKSFS 301 >At5g18510.1 68418.m02185 hypothetical protein Length = 702 Score = 27.9 bits (59), Expect = 4.5 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = -3 Query: 411 ELQEWLLEEVIPHVLCTGKYDPAIKQQEEKNKQLVTKLIA 292 E WLLE+ C GK+ +K++E+ +++L + +A Sbjct: 625 EADSWLLEDREIERRCIGKFCSEVKKEEDVDERLRQRTLA 664 >At1g77960.1 68414.m09085 hypothetical protein Length = 211 Score = 27.9 bits (59), Expect = 4.5 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = +2 Query: 56 NLQQLVFFVHHFIFSLHSFLK 118 N +Q +FF+H F+ + H+F K Sbjct: 176 NAEQRLFFLHFFLLTYHTFFK 196 >At1g24560.1 68414.m03090 expressed protein Length = 678 Score = 27.9 bits (59), Expect = 4.5 Identities = 16/66 (24%), Positives = 34/66 (51%), Gaps = 3/66 (4%) Frame = -2 Query: 220 EFIERIIAIKDKQIEAKDL---QVTRVMTDLNRMYTGFQETMQRKDEMMHKKDELLQVKD 50 E I +IA D++ +A++ + + NR+ E ++++DE ++DE L+ K+ Sbjct: 35 ERITELIAELDREKKAREAAESSKSELQVSFNRLKALAVEAIKKRDESKRERDEALKEKE 94 Query: 49 TQVSNL 32 + L Sbjct: 95 NLTNEL 100 >At3g61380.1 68416.m06869 expressed protein Length = 718 Score = 27.5 bits (58), Expect = 6.0 Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 7/56 (12%) Frame = -3 Query: 342 IKQQEEKNKQLVTKLIA-------TFTEHTNALQAVGRKKPRNFLKSKSLSNASSQ 196 + + EEK K+++ +LIA T + A +G ++ +FLK K LSN +Q Sbjct: 88 VDESEEKFKEIIKRLIAQKEGEIQTCKDLLEAFHVLGSEEEESFLK-KKLSNEDAQ 142 >At3g26540.1 68416.m03313 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile: PF01535 PPR repeat Length = 700 Score = 27.5 bits (58), Expect = 6.0 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = +1 Query: 277 VLGKCRNQFSHQLFVLFLLLFNCGV 351 + G CRN S ++F LF+LL N GV Sbjct: 569 IRGCCRNGRSKEVFELFMLLENEGV 593 >At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss-Prot:O33600) [Sulfolobus acidocaldarius] Length = 440 Score = 27.5 bits (58), Expect = 6.0 Identities = 14/72 (19%), Positives = 37/72 (51%), Gaps = 3/72 (4%) Frame = -2 Query: 247 KTEELFKKQEFIERIIAIKDKQ---IEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMHK 77 + EL K+ E +++ + K+K+ IEA+ + + + +LN +T + + + K Sbjct: 116 RATELEKQVEVLKKFLEQKNKEKELIEAQTSETEKKLNELNSRVEKLHKTNEEQKNKIRK 175 Query: 76 KDELLQVKDTQV 41 + L++ + ++ Sbjct: 176 LERALKISEEEM 187 >At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss-Prot:O33600) [Sulfolobus acidocaldarius] Length = 440 Score = 27.5 bits (58), Expect = 6.0 Identities = 14/72 (19%), Positives = 37/72 (51%), Gaps = 3/72 (4%) Frame = -2 Query: 247 KTEELFKKQEFIERIIAIKDKQ---IEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMHK 77 + EL K+ E +++ + K+K+ IEA+ + + + +LN +T + + + K Sbjct: 116 RATELEKQVEVLKKFLEQKNKEKELIEAQTSETEKKLNELNSRVEKLHKTNEEQKNKIRK 175 Query: 76 KDELLQVKDTQV 41 + L++ + ++ Sbjct: 176 LERALKISEEEM 187 >At1g10880.1 68414.m01250 expressed protein contains Pfam profile PF03267: Arabidopsis protein of unknown function, DUF266 Length = 651 Score = 27.1 bits (57), Expect = 7.9 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +1 Query: 415 GVRKLALHNQLNHARFSNKHCM 480 G R++ +HN LNH N HCM Sbjct: 473 GEREINIHN-LNHQMLHNAHCM 493 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,096,596 Number of Sequences: 28952 Number of extensions: 212542 Number of successful extensions: 633 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 615 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 633 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 993966856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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