SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120416X.Seq
         (536 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g25070.1 68418.m02971 expressed protein                             42   3e-04
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ...    31   0.49 
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ...    31   0.49 
At4g23800.1 68417.m03422 high mobility group (HMG1/2) family pro...    31   0.49 
At1g33820.1 68414.m04185 hypothetical protein                          29   1.5  
At1g15040.2 68414.m01798 glutamine amidotransferase-related            29   1.5  
At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos...    29   2.0  
At5g22040.1 68418.m02566 expressed protein                             29   2.6  
At1g15040.1 68414.m01797 glutamine amidotransferase-related            29   2.6  
At5g18510.1 68418.m02185 hypothetical protein                          28   4.5  
At1g77960.1 68414.m09085 hypothetical protein                          28   4.5  
At1g24560.1 68414.m03090 expressed protein                             28   4.5  
At3g61380.1 68416.m06869 expressed protein                             27   6.0  
At3g26540.1 68416.m03313 pentatricopeptide (PPR) repeat-containi...    27   6.0  
At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 tr...    27   6.0  
At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 tr...    27   6.0  
At1g10880.1 68414.m01250 expressed protein contains Pfam profile...    27   7.9  

>At5g25070.1 68418.m02971 expressed protein
          Length = 736

 Score = 41.9 bits (94), Expect = 3e-04
 Identities = 22/82 (26%), Positives = 41/82 (50%)
 Frame = -2

Query: 250 QKTEELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMHKKD 71
           +K E L  + E +  ++  K+K+I+  D Q+  V   +N + TGF+E     D+M++   
Sbjct: 384 KKKEHLANELEELLALVKAKEKEIDENDSQIEAVEERINNVVTGFKELQTSMDKMLNDVQ 443

Query: 70  ELLQVKDTQVSNLIAKMIDLSD 5
             L   D +  +L  K  D+ +
Sbjct: 444 AGLTEVDKETEDLSRKKKDVDE 465


>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
           protein contains Pfam profile PF01465: GRIP domain;
           supporting cDNA gi|20303028|gb|AF499634.1|
          Length = 765

 Score = 31.1 bits (67), Expect = 0.49
 Identities = 19/86 (22%), Positives = 41/86 (47%), Gaps = 2/86 (2%)
 Frame = -2

Query: 295 CDIYRAHKRAASGGAQKTE-ELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTG 119
           CD  ++    A     + E E+ K +  +   ++++ + +  KD ++     ++NR+ + 
Sbjct: 371 CDALKSKLEIAESNYLQAEIEVAKMRSQLGSEMSMQTQILSTKDAELKGAREEINRLQSE 430

Query: 118 FQETMQRKDEMMHKKD-ELLQVKDTQ 44
           F     R   ++ KKD EL   KD++
Sbjct: 431 FSSYKIRAHALLQKKDMELAAAKDSE 456


>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
           protein contains Pfam profile PF01465: GRIP domain;
           supporting cDNA gi|20303028|gb|AF499634.1|
          Length = 788

 Score = 31.1 bits (67), Expect = 0.49
 Identities = 19/86 (22%), Positives = 41/86 (47%), Gaps = 2/86 (2%)
 Frame = -2

Query: 295 CDIYRAHKRAASGGAQKTE-ELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTG 119
           CD  ++    A     + E E+ K +  +   ++++ + +  KD ++     ++NR+ + 
Sbjct: 371 CDALKSKLEIAESNYLQAEIEVAKMRSQLGSEMSMQTQILSTKDAELKGAREEINRLQSE 430

Query: 118 FQETMQRKDEMMHKKD-ELLQVKDTQ 44
           F     R   ++ KKD EL   KD++
Sbjct: 431 FSSYKIRAHALLQKKDMELAAAKDSE 456


>At4g23800.1 68417.m03422 high mobility group (HMG1/2) family
           protein similar to HMG2B [Homo sapiens] GI:32335;
           contains Pfam profile PF00505: HMG (high mobility group)
           box
          Length = 456

 Score = 31.1 bits (67), Expect = 0.49
 Identities = 19/74 (25%), Positives = 39/74 (52%), Gaps = 6/74 (8%)
 Frame = -2

Query: 235 LFKKQEFIERI---IAIKDKQIEAKDLQVTRVMTDLNRMYTG---FQETMQRKDEMMHKK 74
           L +K E +E     +++K K  ++ +  +  + T L +M       +E ++ KDE++ KK
Sbjct: 20  LKQKNELVETPPSPVSVKGKSAKSFEQDLMEMQTMLEKMKIEKDKTEELLKEKDEILRKK 79

Query: 73  DELLQVKDTQVSNL 32
           +E L+ +D +   L
Sbjct: 80  EEELETRDAEQEKL 93


>At1g33820.1 68414.m04185 hypothetical protein
          Length = 182

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 16/51 (31%), Positives = 27/51 (52%)
 Frame = -2

Query: 172 KDLQVTRVMTDLNRMYTGFQETMQRKDEMMHKKDELLQVKDTQVSNLIAKM 20
           KD Q   ++  +  M T F+E     +  M +  + LQ+KDTQ  N++ +M
Sbjct: 72  KDTQTMNMVKQM--METAFKENAMVHERDMCQLKDTLQLKDTQTINMMTQM 120


>At1g15040.2 68414.m01798 glutamine amidotransferase-related
          Length = 333

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 13/48 (27%), Positives = 27/48 (56%)
 Frame = -2

Query: 223 QEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMH 80
           QEF++ +IA + KQ+ A  +++ R  T L + ++  +    R   ++H
Sbjct: 268 QEFVKAVIAFQKKQVNATQVEMKRKTTTLVKSFSQAEFLEVRDSFVLH 315


>At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative /
           phosphoglyceride transfer protein, putative similar to
           SP:P24859 from [Kluyveromyces lactissimilar to
           phosphatidylinositol transfer-like protein IV
           (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
           CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
           N-terminus
          Length = 608

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 15/48 (31%), Positives = 26/48 (54%)
 Frame = -2

Query: 160 VTRVMTDLNRMYTGFQETMQRKDEMMHKKDELLQVKDTQVSNLIAKMI 17
           ++ V++ L  +    +    RK EM H+K+ELL     +V  L A++I
Sbjct: 526 ISSVLSRLGDLEKQIENLHSRKSEMPHEKEELLNAAVYRVDALEAELI 573


>At5g22040.1 68418.m02566 expressed protein
          Length = 284

 Score = 28.7 bits (61), Expect = 2.6
 Identities = 17/67 (25%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
 Frame = -2

Query: 256 GAQKTEELFKKQEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYTGFQETMQR-KDEMMH 80
           G Q  E L  +Q+F++ I  +   QIEA+D ++ R    +N +   ++E +   +     
Sbjct: 120 GVQTFEGL--EQKFMDDITRLAKDQIEAEDAEIARHREKINTINARYEEQLATLRARHTG 177

Query: 79  KKDELLQ 59
           K++E+++
Sbjct: 178 KREEIMR 184


>At1g15040.1 68414.m01797 glutamine amidotransferase-related
          Length = 395

 Score = 28.7 bits (61), Expect = 2.6
 Identities = 11/34 (32%), Positives = 22/34 (64%)
 Frame = -2

Query: 223 QEFIERIIAIKDKQIEAKDLQVTRVMTDLNRMYT 122
           QEF++ +IA + KQ+ A  +++ R  T L + ++
Sbjct: 268 QEFVKAVIAFQKKQVNATQVEMKRKTTTLVKSFS 301


>At5g18510.1 68418.m02185 hypothetical protein
          Length = 702

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 12/40 (30%), Positives = 23/40 (57%)
 Frame = -3

Query: 411 ELQEWLLEEVIPHVLCTGKYDPAIKQQEEKNKQLVTKLIA 292
           E   WLLE+      C GK+   +K++E+ +++L  + +A
Sbjct: 625 EADSWLLEDREIERRCIGKFCSEVKKEEDVDERLRQRTLA 664


>At1g77960.1 68414.m09085 hypothetical protein
          Length = 211

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 9/21 (42%), Positives = 15/21 (71%)
 Frame = +2

Query: 56  NLQQLVFFVHHFIFSLHSFLK 118
           N +Q +FF+H F+ + H+F K
Sbjct: 176 NAEQRLFFLHFFLLTYHTFFK 196


>At1g24560.1 68414.m03090 expressed protein
          Length = 678

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 16/66 (24%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
 Frame = -2

Query: 220 EFIERIIAIKDKQIEAKDL---QVTRVMTDLNRMYTGFQETMQRKDEMMHKKDELLQVKD 50
           E I  +IA  D++ +A++      + +    NR+     E ++++DE   ++DE L+ K+
Sbjct: 35  ERITELIAELDREKKAREAAESSKSELQVSFNRLKALAVEAIKKRDESKRERDEALKEKE 94

Query: 49  TQVSNL 32
              + L
Sbjct: 95  NLTNEL 100


>At3g61380.1 68416.m06869 expressed protein
          Length = 718

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 7/56 (12%)
 Frame = -3

Query: 342 IKQQEEKNKQLVTKLIA-------TFTEHTNALQAVGRKKPRNFLKSKSLSNASSQ 196
           + + EEK K+++ +LIA       T  +   A   +G ++  +FLK K LSN  +Q
Sbjct: 88  VDESEEKFKEIIKRLIAQKEGEIQTCKDLLEAFHVLGSEEEESFLK-KKLSNEDAQ 142


>At3g26540.1 68416.m03313 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile: PF01535 PPR repeat
          Length = 700

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +1

Query: 277 VLGKCRNQFSHQLFVLFLLLFNCGV 351
           + G CRN  S ++F LF+LL N GV
Sbjct: 569 IRGCCRNGRSKEVFELFMLLENEGV 593


>At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2
           transmembrane domains; similar to DNA double-strand
           break repair rad50 ATPase. (Swiss-Prot:O33600)
           [Sulfolobus acidocaldarius]
          Length = 440

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 14/72 (19%), Positives = 37/72 (51%), Gaps = 3/72 (4%)
 Frame = -2

Query: 247 KTEELFKKQEFIERIIAIKDKQ---IEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMHK 77
           +  EL K+ E +++ +  K+K+   IEA+  +  + + +LN       +T + +   + K
Sbjct: 116 RATELEKQVEVLKKFLEQKNKEKELIEAQTSETEKKLNELNSRVEKLHKTNEEQKNKIRK 175

Query: 76  KDELLQVKDTQV 41
            +  L++ + ++
Sbjct: 176 LERALKISEEEM 187


>At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2
           transmembrane domains; similar to DNA double-strand
           break repair rad50 ATPase. (Swiss-Prot:O33600)
           [Sulfolobus acidocaldarius]
          Length = 440

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 14/72 (19%), Positives = 37/72 (51%), Gaps = 3/72 (4%)
 Frame = -2

Query: 247 KTEELFKKQEFIERIIAIKDKQ---IEAKDLQVTRVMTDLNRMYTGFQETMQRKDEMMHK 77
           +  EL K+ E +++ +  K+K+   IEA+  +  + + +LN       +T + +   + K
Sbjct: 116 RATELEKQVEVLKKFLEQKNKEKELIEAQTSETEKKLNELNSRVEKLHKTNEEQKNKIRK 175

Query: 76  KDELLQVKDTQV 41
            +  L++ + ++
Sbjct: 176 LERALKISEEEM 187


>At1g10880.1 68414.m01250 expressed protein contains Pfam profile
           PF03267: Arabidopsis protein of unknown function, DUF266
          Length = 651

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +1

Query: 415 GVRKLALHNQLNHARFSNKHCM 480
           G R++ +HN LNH    N HCM
Sbjct: 473 GEREINIHN-LNHQMLHNAHCM 493


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,096,596
Number of Sequences: 28952
Number of extensions: 212542
Number of successful extensions: 633
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 615
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 633
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -