Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120389.Seq
(661 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14570.2 68414.m01733 UBX domain-containing protein contains ... 31 0.68
At1g14570.1 68414.m01732 UBX domain-containing protein contains ... 31 0.68
At3g04680.2 68416.m00502 pre-mRNA cleavage complex family protei... 29 2.1
At3g04680.1 68416.m00501 pre-mRNA cleavage complex family protei... 29 2.1
At2g45500.1 68415.m05659 AAA-type ATPase family protein similar ... 29 2.1
At5g61830.1 68418.m07758 short-chain dehydrogenase/reductase (SD... 29 3.6
At5g57380.1 68418.m07169 fibronectin type III domain-containing ... 29 3.6
At2g46560.1 68415.m05808 transducin family protein / WD-40 repea... 29 3.6
At5g60920.1 68418.m07642 phytochelatin synthetase, putative / CO... 28 4.8
At3g02210.1 68416.m00202 phytochelatin synthetase family protein... 28 6.3
At2g39810.1 68415.m04890 expressed protein 28 6.3
At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop... 27 8.4
At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop... 27 8.4
>At1g14570.2 68414.m01733 UBX domain-containing protein contains
Pfam profiles PF00789: UBX domain, PF02809: Ubiquitin
interaction motif
Length = 468
Score = 31.1 bits (67), Expect = 0.68
Identities = 20/71 (28%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = +1
Query: 217 GLLDPKRNTSAVQEAINTLKHTMNTEDVCAHML--DIVSFERIKEYIRANLGHFTVITDK 390
G + + TS+ Q+ L + +T + +HML D + + + + I+AN + V D
Sbjct: 185 GSFEQAKTTSSSQDKW-LLVNLQSTTEFSSHMLNRDTWANDAVSQTIKANFIFWQVYDDT 243
Query: 391 CSKRKVCLHHK 423
RKVC ++K
Sbjct: 244 TEGRKVCTYYK 254
>At1g14570.1 68414.m01732 UBX domain-containing protein contains
Pfam profiles PF00789: UBX domain, PF02809: Ubiquitin
interaction motif
Length = 468
Score = 31.1 bits (67), Expect = 0.68
Identities = 20/71 (28%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = +1
Query: 217 GLLDPKRNTSAVQEAINTLKHTMNTEDVCAHML--DIVSFERIKEYIRANLGHFTVITDK 390
G + + TS+ Q+ L + +T + +HML D + + + + I+AN + V D
Sbjct: 185 GSFEQAKTTSSSQDKW-LLVNLQSTTEFSSHMLNRDTWANDAVSQTIKANFIFWQVYDDT 243
Query: 391 CSKRKVCLHHK 423
RKVC ++K
Sbjct: 244 TEGRKVCTYYK 254
>At3g04680.2 68416.m00502 pre-mRNA cleavage complex family protein
similar to Pre-mRNA cleavage complex II protein Clp1
(Swiss-Prot:Q92989) [Homo sapiens]
Length = 444
Score = 29.5 bits (63), Expect = 2.1
Identities = 30/113 (26%), Positives = 55/113 (48%), Gaps = 8/113 (7%)
Frame = +1
Query: 94 VRSIKKRFDNRQLQDFFYATLKKID---NNDAPQKLHALRRV*FGLLDPKRNTSAVQEAI 264
V+ ++KR N ++Q++FY K++ N + L R+ G PK A ++
Sbjct: 307 VKEVRKRSRNFKIQEYFYGLSKELSPYANTSSFSDLQVF-RIGGGPQAPKSALPAGSTSV 365
Query: 265 -NTLKHT-MNTED-VCAHMLDIVSF-ERIKEYIRANLGHFTVITD-KCSKRKV 408
N L+ T +N +D H + VS+ E + I +N+ F +T+ K+K+
Sbjct: 366 SNPLRVTPVNIDDRDLLHSVLAVSYAEEPDQIISSNVSGFVYVTEVNVQKKKI 418
>At3g04680.1 68416.m00501 pre-mRNA cleavage complex family protein
similar to Pre-mRNA cleavage complex II protein Clp1
(Swiss-Prot:Q92989) [Homo sapiens]
Length = 444
Score = 29.5 bits (63), Expect = 2.1
Identities = 30/113 (26%), Positives = 55/113 (48%), Gaps = 8/113 (7%)
Frame = +1
Query: 94 VRSIKKRFDNRQLQDFFYATLKKID---NNDAPQKLHALRRV*FGLLDPKRNTSAVQEAI 264
V+ ++KR N ++Q++FY K++ N + L R+ G PK A ++
Sbjct: 307 VKEVRKRSRNFKIQEYFYGLSKELSPYANTSSFSDLQVF-RIGGGPQAPKSALPAGSTSV 365
Query: 265 -NTLKHT-MNTED-VCAHMLDIVSF-ERIKEYIRANLGHFTVITD-KCSKRKV 408
N L+ T +N +D H + VS+ E + I +N+ F +T+ K+K+
Sbjct: 366 SNPLRVTPVNIDDRDLLHSVLAVSYAEEPDQIISSNVSGFVYVTEVNVQKKKI 418
>At2g45500.1 68415.m05659 AAA-type ATPase family protein similar to
SP|Q9QYY8 Spastin (Fragment) {Mus musculus}; contains
Pfam profiles PF00004: ATPase AAA family, PF04212: MIT
domain
Length = 487
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +2
Query: 416 ITNELPGCWALKKYIHQEYKRVVSKVYKNQTGKHAGATIFYRNCGRVQNE 565
+TNE + LK Y + + V + G H A + YRN R+ NE
Sbjct: 43 VTNERIA-YKLKGYFDLAKEEIAKGVRAEEWGLHDDALLHYRNAQRIMNE 91
>At5g61830.1 68418.m07758 short-chain dehydrogenase/reductase (SDR)
family protein contains INTERPRO family IPR002198 short
chain dehydrogenase/reductase SDR family
Length = 316
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 235 RNTSAVQEAINTLKHTMNTEDVCAHMLDIVSFERIKEY 348
RN +A EA+ +L+H V H LD+ I+E+
Sbjct: 68 RNVNAGLEAVKSLRHQEEGLKVYFHQLDVTDSSSIREF 105
>At5g57380.1 68418.m07169 fibronectin type III domain-containing
protein / PHD finger protein-related contains Pfam
profiles PF00041: Fibronectin type III domain, PF00628:
PHD-finger
Length = 600
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +2
Query: 422 NELPGCWALKKYIHQEYKRVVSKVYKNQTG-KHAGATIFYRNCGRVQNEKLLK*TG 586
N+L GCW + + +E +RV Y+ G K T YRN + +E + K G
Sbjct: 187 NDLLGCWRKQVKVAKETRRVDVLCYRLSLGQKLLRGTTKYRNLLELMDEAVKKLEG 242
>At2g46560.1 68415.m05808 transducin family protein / WD-40 repeat
family protein similar to CPY (GI:3096961) {Chironomus
thummi}; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies, 3 weak)|9780477|gb|BE522499.1|BE522499
Length = 2471
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/36 (38%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +2
Query: 485 SKVYKNQTGKHAGATIFYRNCGRVQNEKL-LK*TGI 589
S VY N+T +H + ++ CGR+QN++ LK T +
Sbjct: 515 SSVYLNRTIEHPISVASWKPCGRLQNQEFRLKYTSL 550
>At5g60920.1 68418.m07642 phytochelatin synthetase, putative / COBRA
cell expansion protein COB, putative similar to
phytochelatin synthetase GI:29570314; similar to
GB:AAK56072; identified in Roudier, et al, Plant Phys.
(2002) 130:538-548 (PMID:12376623); identical to cDNA
putative phytochelatin synthetase GI:3559804; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 456
Score = 28.3 bits (60), Expect = 4.8
Identities = 17/41 (41%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 644 KIYNSNL--AGPCWNLLSRARFQFISTTFHFAHGRSFCRRL 528
K YN L AGP N+ S F+ +TF F G +F RR+
Sbjct: 371 KFYNDFLSEAGPLGNVQSEILFRKDQSTFTFEKGWAFPRRI 411
>At3g02210.1 68416.m00202 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL3 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623);
supported by cDNA: gi|26452134|dbj|AK118555.1; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 452
Score = 27.9 bits (59), Expect = 6.3
Identities = 17/41 (41%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 644 KIYNSNL--AGPCWNLLSRARFQFISTTFHFAHGRSFCRRL 528
K YN L AGP N+ S FQ ++ F F G +F RR+
Sbjct: 366 KFYNDLLMQAGPFGNVQSELLFQKEASAFTFEKGWAFPRRI 406
>At2g39810.1 68415.m04890 expressed protein
Length = 865
Score = 27.9 bits (59), Expect = 6.3
Identities = 19/68 (27%), Positives = 29/68 (42%), Gaps = 7/68 (10%)
Frame = +1
Query: 229 PKRNTSAVQEAIN-----TLKHTMNTEDV--CAHMLDIVSFERIKEYIRANLGHFTVITD 387
P ++ VQEA+ L+ N V C D+ S R Y+ GH ++ T+
Sbjct: 22 PNYSSKPVQEALKHLASINLRELCNEAKVERCRATRDLASCGRFVNYVLNPCGHASLCTE 81
Query: 388 KCSKRKVC 411
C + VC
Sbjct: 82 CCQRCDVC 89
>At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 410 VFITNELPGCWALKKYIHQEYKRVVSKVYKNQTGKHAGATIFY 538
VF+ N LP AL I++E+K +Y +G++ FY
Sbjct: 79 VFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSFY 121
>At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 410 VFITNELPGCWALKKYIHQEYKRVVSKVYKNQTGKHAGATIFY 538
VF+ N LP AL I++E+K +Y +G++ FY
Sbjct: 79 VFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSFY 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,270,084
Number of Sequences: 28952
Number of extensions: 292470
Number of successful extensions: 722
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 722
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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