Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120376.Seq
(812 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g15430.1 68415.m01765 DNA-directed RNA polymerase II 36 kDa p... 85 4e-17
At2g15400.1 68415.m01762 DNA-directed RNA polymerase II 36 kDa p... 76 3e-14
At1g60850.2 68414.m06849 DNA-directed RNA polymerase, putative i... 52 3e-07
At1g60850.1 68414.m06848 DNA-directed RNA polymerase, putative i... 52 3e-07
At1g60620.1 68414.m06824 DNA-directed RNA polymerase, putative i... 50 1e-06
At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative i... 50 2e-06
At1g17750.1 68414.m02197 leucine-rich repeat transmembrane prote... 32 0.52
At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma m... 30 2.1
At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 29 2.8
At4g01280.1 68417.m00169 myb family transcription factor contain... 29 3.7
At1g01580.1 68414.m00075 ferric-chelate reductase, putative simi... 29 4.9
At3g52490.1 68416.m05772 heat shock protein-related contains sim... 28 6.4
At1g50290.1 68414.m05638 expressed protein 28 6.4
At1g55610.1 68414.m06365 protein kinase family protein contains ... 28 8.5
>At2g15430.1 68415.m01765 DNA-directed RNA polymerase II 36 kDa
polypeptide A / RNA polymerase II subunit 3 (RPB36A)
identical to SP|Q39211 DNA-directed RNA polymerase II 36
kDa polypeptide A (EC 2.7.7.6) (RNA polymerase II
subunit 3) {Arabidopsis thaliana}
Length = 319
Score = 85.4 bits (202), Expect = 4e-17
Identities = 43/82 (52%), Positives = 55/82 (67%), Gaps = 3/82 (3%)
Frame = +1
Query: 250 VRANSTVLSDEFLAHRIGLIPLISDDVVDKIRYSRDCMCVDF---CSECSVEFTLDVKCT 420
+ NS+VL+DEF+AHR+GLIPL S+ + +R+SRDC D C CSVEF L KC
Sbjct: 55 IEVNSSVLNDEFIAHRLGLIPLTSERAMS-MRFSRDCDACDGDGQCEFCSVEFRLSSKCV 113
Query: 421 DEQTRHVTTADLKSSDPRVVPV 486
+QT VT+ DL S+DP V PV
Sbjct: 114 TDQTLDVTSRDLYSADPTVTPV 135
Score = 74.5 bits (175), Expect = 7e-14
Identities = 33/59 (55%), Positives = 44/59 (74%)
Frame = +3
Query: 507 SQADYGEADEILIIKLRKGQELKLRAYAKKGFGKEHAKWNPTAGVSFEYDPDNIMRHTL 683
S +D E I+I+KLR+GQELKLRA A+KG GK+HAKW+P A V+F Y+PD I+ +
Sbjct: 142 SVSDSSEHKGIIIVKLRRGQELKLRAIARKGIGKDHAKWSPAATVTFMYEPDIIINEDM 200
Score = 33.9 bits (74), Expect = 0.13
Identities = 13/27 (48%), Positives = 21/27 (77%)
Frame = +2
Query: 179 IKRTNSMRRVFIAETPTMAIDWVQLEL 259
+ N++RRV I+E PT+AID V++E+
Sbjct: 31 VSMANALRRVMISEVPTVAIDLVEIEV 57
>At2g15400.1 68415.m01762 DNA-directed RNA polymerase II 36 kDa
polypeptide B / RNA polymerase II subunit 3 (RPB36B)
identical to SP|Q39212 DNA-directed RNA polymerase II 36
kDa polypeptide B (EC 2.7.7.6) (RNA polymerase II
subunit 3) {Arabidopsis thaliana}
Length = 319
Score = 75.8 bits (178), Expect = 3e-14
Identities = 39/82 (47%), Positives = 53/82 (64%), Gaps = 3/82 (3%)
Frame = +1
Query: 250 VRANSTVLSDEFLAHRIGLIPLISDDVVDKIRYSRDCM-CV--DFCSECSVEFTLDVKCT 420
+ NS+VL+DEF+A R+ LIPL S+ + +R+ +DC C + C CSVEF L KC
Sbjct: 55 IEVNSSVLNDEFIAQRLSLIPLTSERAMS-MRFCQDCEDCNGDEHCEFCSVEFPLSAKCV 113
Query: 421 DEQTRHVTTADLKSSDPRVVPV 486
+QT VT+ DL S+DP V PV
Sbjct: 114 TDQTLDVTSRDLYSADPTVTPV 135
Score = 70.9 bits (166), Expect = 9e-13
Identities = 31/59 (52%), Positives = 43/59 (72%)
Frame = +3
Query: 507 SQADYGEADEILIIKLRKGQELKLRAYAKKGFGKEHAKWNPTAGVSFEYDPDNIMRHTL 683
S +D E I+I KLR+GQELKL+A A+KG GK+HAKW+P A V++ Y+PD I+ +
Sbjct: 142 STSDSSEHKGIIIAKLRRGQELKLKALARKGIGKDHAKWSPAATVTYMYEPDIIINEEM 200
Score = 32.3 bits (70), Expect = 0.39
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +2
Query: 179 IKRTNSMRRVFIAETPTMAIDWVQLEL 259
+ N++RRV I+E PTMAI V++E+
Sbjct: 31 VSMANALRRVMISEVPTMAIHLVKIEV 57
>At1g60850.2 68414.m06849 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514322; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 375
Score = 52.4 bits (120), Expect = 3e-07
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +3
Query: 534 EILIIKLRKGQELKLRAYAKKGFGKEHAKWNPTAGVSFEYDPDNIMR 674
+ILI KL GQE++L A+A KG GK HAKW+P + P+ ++R
Sbjct: 231 DILIAKLAPGQEIELEAHAVKGIGKTHAKWSPVGTAWYRMHPEVVLR 277
Score = 39.5 bits (88), Expect = 0.003
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +1
Query: 259 NSTVLSDEFLAHRIGLIPLISDDVVDKIRYSRDCMCVDFCSECSVEFTLDVKCTDEQTR- 435
N++V+ DE LAHR+GLIP+ +D + + D + ++ F L VKC + R
Sbjct: 122 NTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHD----QANEKNTIVFKLHVKCPKNRPRL 177
Query: 436 HVTTADLK 459
V T+DLK
Sbjct: 178 KVLTSDLK 185
>At1g60850.1 68414.m06848 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514322; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 375
Score = 52.4 bits (120), Expect = 3e-07
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +3
Query: 534 EILIIKLRKGQELKLRAYAKKGFGKEHAKWNPTAGVSFEYDPDNIMR 674
+ILI KL GQE++L A+A KG GK HAKW+P + P+ ++R
Sbjct: 231 DILIAKLAPGQEIELEAHAVKGIGKTHAKWSPVGTAWYRMHPEVVLR 277
Score = 39.5 bits (88), Expect = 0.003
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +1
Query: 259 NSTVLSDEFLAHRIGLIPLISDDVVDKIRYSRDCMCVDFCSECSVEFTLDVKCTDEQTR- 435
N++V+ DE LAHR+GLIP+ +D + + D + ++ F L VKC + R
Sbjct: 122 NTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHD----QANEKNTIVFKLHVKCPKNRPRL 177
Query: 436 HVTTADLK 459
V T+DLK
Sbjct: 178 KVLTSDLK 185
>At1g60620.1 68414.m06824 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514324; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 385
Score = 50.4 bits (115), Expect = 1e-06
Identities = 22/46 (47%), Positives = 31/46 (67%)
Frame = +3
Query: 534 EILIIKLRKGQELKLRAYAKKGFGKEHAKWNPTAGVSFEYDPDNIM 671
+ILI KL GQE++L A+A KG GK HAKW+P A + P+ ++
Sbjct: 238 DILIAKLGPGQEIELEAHAVKGIGKTHAKWSPVATAWYRMLPEVVL 283
Score = 38.7 bits (86), Expect = 0.005
Identities = 25/71 (35%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
Frame = +1
Query: 250 VRANSTVLSDEFLAHRIGLIPLISDDVVDKIRYSRDCMCVDFCSECSVEFTLDVKC-TDE 426
V N++V+ DE LAHR+GLIP+ +D + + D + ++ F L VKC +
Sbjct: 126 VANNTSVIQDEVLAHRLGLIPIAADPRLFEYLSEND----QPNEKNTIVFKLHVKCLKGD 181
Query: 427 QTRHVTTADLK 459
R V T++LK
Sbjct: 182 PRRKVLTSELK 192
>At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514322; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 302
Score = 49.6 bits (113), Expect = 2e-06
Identities = 21/43 (48%), Positives = 28/43 (65%)
Frame = +3
Query: 534 EILIIKLRKGQELKLRAYAKKGFGKEHAKWNPTAGVSFEYDPD 662
+ILI KL GQE++L A+A KG GK HAKW+P + P+
Sbjct: 231 DILIAKLAPGQEIELEAHAVKGIGKTHAKWSPVGTAWYRMHPE 273
Score = 39.5 bits (88), Expect = 0.003
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +1
Query: 259 NSTVLSDEFLAHRIGLIPLISDDVVDKIRYSRDCMCVDFCSECSVEFTLDVKCTDEQTR- 435
N++V+ DE LAHR+GLIP+ +D + + D + ++ F L VKC + R
Sbjct: 122 NTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHD----QANEKNTIVFKLHVKCPKNRPRL 177
Query: 436 HVTTADLK 459
V T+DLK
Sbjct: 178 KVLTSDLK 185
>At1g17750.1 68414.m02197 leucine-rich repeat transmembrane protein
kinase, putative similar to receptor-like protein kinase
INRPK1 GI:1684913 from [Ipomoea nil]
Length = 1088
Score = 31.9 bits (69), Expect = 0.52
Identities = 23/84 (27%), Positives = 38/84 (45%), Gaps = 1/84 (1%)
Frame = -1
Query: 506 SSLCRDVTGTTLGSLDFKSAVVTCLV-CSSVHFTSNVNSTLHSEQKSTHMQSREYLILST 330
SS C+ + L DF+ V + CSS+H V L S+ R+ ++
Sbjct: 240 SSNCKKLVSLDLSFNDFQGGVPPEIGNCSSLHSLVMVKCNLTGTIPSSMGMLRKVSVIDL 299
Query: 329 TSSDMSGMRPMRCAKNSSLKTVEL 258
+ + +SG P SSL+T++L
Sbjct: 300 SDNRLSGNIPQELGNCSSLETLKL 323
>At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma
membrane-type / Ca(2+)-ATPase isoform 2 (ACA2) identical
to SP|O81108 Calcium-transporting ATPase 2, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2)
{Arabidopsis thaliana}
Length = 1014
Score = 29.9 bits (64), Expect = 2.1
Identities = 16/47 (34%), Positives = 28/47 (59%)
Frame = -1
Query: 518 IGLASSLCRDVTGTTLGSLDFKSAVVTCLVCSSVHFTSNVNSTLHSE 378
+G A+++C D TGT + + + V +C +C +V +N S+L SE
Sbjct: 445 MGSATTICSDKTGTL--TTNHMTVVKSC-ICMNVQDVANKGSSLQSE 488
>At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA7)
identical to SP|O64806 Potential calcium-transporting
ATPase 7, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong
similarity to SP|O81108 Calcium-transporting ATPase 2,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
2) {Arabidopsis thaliana}
Length = 1015
Score = 29.5 bits (63), Expect = 2.8
Identities = 17/68 (25%), Positives = 37/68 (54%)
Frame = -1
Query: 518 IGLASSLCRDVTGTTLGSLDFKSAVVTCLVCSSVHFTSNVNSTLHSEQKSTHMQSREYLI 339
+G A+++C D TGT + + + V +C +C +V ++ +S+L S+ ++ LI
Sbjct: 446 MGSATTICSDKTGTL--TTNHMTVVKSC-ICMNVQDVASKSSSLQSDIPEAALKLLLQLI 502
Query: 338 LSTTSSDM 315
+ T ++
Sbjct: 503 FNNTGGEV 510
>At4g01280.1 68417.m00169 myb family transcription factor contains
PFAM profile: PF00249 myb-like DNA binding domain
Length = 302
Score = 29.1 bits (62), Expect = 3.7
Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = +3
Query: 597 GFGKEHAKWNPTAGVSFEYDPDNIMR--HTLYPKPDEWPKSEHTELNEDQYEADFNWER 767
GFG A T+ VSF DP +R +T+ + W EH + E + D +W++
Sbjct: 26 GFGSIPATGR-TSTVSFSEDPTTKIRKPYTIKKSRENWTDQEHDKFLEALHLFDRDWKK 83
>At1g01580.1 68414.m00075 ferric-chelate reductase, putative similar
to ferric-chelate reductase (FRO1) [Pisum sativum]
GI:15341529; contains Pfam profile: PF01794 ferric
reductase like transmembrane component
Length = 725
Score = 28.7 bits (61), Expect = 4.9
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 485 TGTTLGSLDFKSAVVTCLVCSSVHFTSNVNSTLHSEQKS 369
TGTTL F VV CL C +HF N S H ++++
Sbjct: 71 TGTTLFMYMFPMMVVACLGCVYLHF-KNRKSPHHIDRET 108
>At3g52490.1 68416.m05772 heat shock protein-related contains
similarity to 101 kDa heat shock protein; HSP101
[Triticum aestivum] gi|11561808|gb|AAC83689
Length = 815
Score = 28.3 bits (60), Expect = 6.4
Identities = 15/46 (32%), Positives = 25/46 (54%)
Frame = +1
Query: 319 SDDVVDKIRYSRDCMCVDFCSECSVEFTLDVKCTDEQTRHVTTADL 456
S++V +++ S D + FC ECSV+F + + +VTT L
Sbjct: 387 SENVSLQLQQSSDQL--SFCEECSVKFESEARFLKSSNSNVTTVAL 430
>At1g50290.1 68414.m05638 expressed protein
Length = 134
Score = 28.3 bits (60), Expect = 6.4
Identities = 13/53 (24%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
Frame = -1
Query: 236 WPLLVFQR*IRVSY--CLYA*FGIFHNEFYIFIR*FCYVNRGLICVRHFLFLK 84
W L++ +R+S+ C++ F + + + R FC+ + G+ C R ++F++
Sbjct: 61 WRLILGHNSLRLSFRICIFRKFDL--QNYLKYERKFCFQHIGIACSRFYMFME 111
>At1g55610.1 68414.m06365 protein kinase family protein contains
Prosite:PS00107: Protein kinases ATP-binding region
signature
Length = 1166
Score = 27.9 bits (59), Expect = 8.5
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = -1
Query: 425 SSVHFTSNVNSTLHSEQKSTHMQSREYLILSTTSSDMSGMRPMRCAKNSSLKTVELA 255
SS FT NV S S Q S ++ + ++ +SG PM K SLKT++L+
Sbjct: 383 SSNGFTGNVPSGFCSLQSSPVLEK-----ILIANNYLSGTVPMELGKCKSLKTIDLS 434
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,127,914
Number of Sequences: 28952
Number of extensions: 368173
Number of successful extensions: 890
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 850
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 888
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1853336000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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