BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120364.Seq (804 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 96 6e-21 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 48 1e-06 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 46 8e-06 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 46 8e-06 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 44 3e-05 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 43 4e-05 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 39 7e-04 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 37 0.003 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 36 0.007 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 36 0.007 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 36 0.009 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 34 0.021 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 32 0.11 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.77 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.8 SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 27 2.4 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 5.5 SPAP8A3.10 |||mitochondrial intermembrane space protein sorting ... 26 5.5 SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase Gmh1|Schizosa... 26 5.5 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 9.5 SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 25 9.5 SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo... 25 9.5 SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces p... 25 9.5 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 95.9 bits (228), Expect = 6e-21 Identities = 41/95 (43%), Positives = 62/95 (65%) Frame = +3 Query: 225 KGSGRWNHFLGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFI 404 KG G + GL + DD+TL+ W I+GP + +ENR+YSLKI C YPD PP F+ Sbjct: 19 KGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFV 78 Query: 405 SRIHMNCVNSQTGLVDNRQVPILARWQRDYTIKTV 509 SRI++ V+ +TG V+ ++ L W+R+Y+++TV Sbjct: 79 SRINLPGVDGETGKVNPHKIDCLRHWKREYSMETV 113 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/27 (62%), Positives = 24/27 (88%) Frame = +1 Query: 181 VPRNFRLLEELEQGQKGVGDGTISWVL 261 VPRNF+LLEELE+G+KG+G+ + S+ L Sbjct: 4 VPRNFKLLEELEKGEKGLGESSCSYGL 30 Score = 31.1 bits (67), Expect = 0.19 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = +2 Query: 509 LQEVRRLMTLKDNMKLSQPPEGSTF 583 L ++++ M N KL QPPEGSTF Sbjct: 114 LLDLKKEMASSSNRKLPQPPEGSTF 138 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 48.4 bits (110), Expect = 1e-06 Identities = 28/76 (36%), Positives = 41/76 (53%), Gaps = 1/76 (1%) Frame = +3 Query: 282 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 461 ++H GM GP TPYE + + IE YP PP F ++I+ V+SQTG + Sbjct: 33 ISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAI---C 89 Query: 462 VPILA-RWQRDYTIKT 506 + IL +W YT+K+ Sbjct: 90 LDILKDQWSPVYTMKS 105 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 45.6 bits (103), Expect = 8e-06 Identities = 26/82 (31%), Positives = 36/82 (43%), Gaps = 1/82 (1%) Frame = +3 Query: 267 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQTG 443 D D L HW G I GP T YE + + + YP PPT F S + H N S Sbjct: 53 DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNI 112 Query: 444 LVDNRQVPILARWQRDYTIKTV 509 +D + +W Y ++T+ Sbjct: 113 CLD----ILKDKWSAVYNVQTI 130 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 45.6 bits (103), Expect = 8e-06 Identities = 20/51 (39%), Positives = 28/51 (54%) Frame = +3 Query: 282 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNS 434 L HW I+GP +PY ++ L I T YP +PP F +RI+ +NS Sbjct: 30 LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINS 80 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 43.6 bits (98), Expect = 3e-05 Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 1/74 (1%) Frame = +3 Query: 291 WTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQTGLVDNRQVP 467 W +IIGP TP+E+ + L + +YP++PP +F+S + H N + +D Q Sbjct: 36 WNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQ-- 93 Query: 468 ILARWQRDYTIKTV 509 RW Y + + Sbjct: 94 --NRWSPTYDVAAI 105 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 43.2 bits (97), Expect = 4e-05 Identities = 23/65 (35%), Positives = 33/65 (50%), Gaps = 1/65 (1%) Frame = +3 Query: 231 SGRWNHFLGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISR 410 +G + L L+ D+ + HW +I GP TPYE + L I YP PP+ F ++ Sbjct: 17 AGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTK 76 Query: 411 I-HMN 422 I H N Sbjct: 77 IVHPN 81 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 39.1 bits (87), Expect = 7e-04 Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 1/60 (1%) Frame = +3 Query: 264 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQT 440 SD + L +W I G P+T +E +Y L + YP PP RF + H N S T Sbjct: 32 SDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGT 91 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 37.1 bits (82), Expect = 0.003 Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = +3 Query: 270 DDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 422 DD ++ W MIIGP T YE + + YP PP +F + I H N Sbjct: 31 DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPN 82 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 35.9 bits (79), Expect = 0.007 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = +3 Query: 282 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 407 + W +I GPP TPYE Y + YP +PP R I+ Sbjct: 34 ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMIT 75 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 35.9 bits (79), Expect = 0.007 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +3 Query: 273 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 416 D L HW +GP + Y + ++ YP +PPT F +RI+ Sbjct: 33 DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 35.5 bits (78), Expect = 0.009 Identities = 17/46 (36%), Positives = 26/46 (56%) Frame = +3 Query: 264 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARF 401 S++D T W +I GP TP+E +Y ++ + YP PPT +F Sbjct: 30 SNEDDFFT-WDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKF 74 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 34.3 bits (75), Expect = 0.021 Identities = 14/47 (29%), Positives = 26/47 (55%) Frame = +3 Query: 309 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 449 GP TPY ++ + +E + YP + P+ F++RI ++ +G V Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 31.9 bits (69), Expect = 0.11 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +3 Query: 309 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 416 GP ++ YE + L++ YP PP RF+++I+ Sbjct: 40 GPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIY 75 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 29.1 bits (62), Expect = 0.77 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = -3 Query: 121 KLRLIQSSFIA*ALPKQIRFI*SKYGNLQQSN 26 K RL++S F +LP Q+RF+ Y NL+ N Sbjct: 27 KFRLLESQFALYSLPCQLRFV--HYNNLEDPN 56 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.9 bits (59), Expect = 1.8 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -2 Query: 479 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 384 S+ NW S+++ SL D + NP+NK W Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322 >SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 27.5 bits (58), Expect = 2.4 Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Frame = -2 Query: 299 SSPMSKSHIIIAFKTQEMVPSPTPFWPCSSS-SKRRKF 189 SSPMS+SH+ I+ + S P P S + K RKF Sbjct: 439 SSPMSESHVQISSEMPRTGMSALPMRPRSQNVDKHRKF 476 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 26.2 bits (55), Expect = 5.5 Identities = 9/37 (24%), Positives = 19/37 (51%) Frame = +3 Query: 306 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 416 I P Y+ + +I+ YP +PP + +++I+ Sbjct: 58 IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94 >SPAP8A3.10 |||mitochondrial intermembrane space protein sorting protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 171 Score = 26.2 bits (55), Expect = 5.5 Identities = 14/28 (50%), Positives = 20/28 (71%) Frame = +1 Query: 442 DLWIIDKSQFLLDGSVTIPSKQSSRSET 525 DL ++KS ++L+ SV PSKQ +SET Sbjct: 68 DLLNVNKS-YILERSVIDPSKQELKSET 94 >SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase Gmh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 26.2 bits (55), Expect = 5.5 Identities = 10/34 (29%), Positives = 19/34 (55%) Frame = -2 Query: 521 SLLEDCFDGIVTLPSSKNWDLSIIHKSSLTIDTV 420 +LL+DCF + +N++ ++ SSL + V Sbjct: 103 NLLDDCFKNRIEYAKLQNYNFEFVNVSSLVVPPV 136 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 25.4 bits (53), Expect = 9.5 Identities = 11/36 (30%), Positives = 16/36 (44%) Frame = -2 Query: 317 WWANNHSSPMSKSHIIIAFKTQEMVPSPTPFWPCSS 210 W + + + H+I + E P PTPF P S Sbjct: 671 WRGEYYPAKKGEYHMIYSALQSERFPGPTPFSPFRS 706 >SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 25.4 bits (53), Expect = 9.5 Identities = 20/65 (30%), Positives = 27/65 (41%), Gaps = 1/65 (1%) Frame = -2 Query: 293 PMSKSHIIIAFKTQEMVPSPTPFWPCSSSSKRRKFRGTTTPDDGFAMITVSPA*RTVK-T 117 P+S S ++F T T P SSSS +T D F +T + + T Sbjct: 247 PVSTSEATVSFSTDIPATPSTLSSPASSSSSYLVETSSTLTDSVFTTVTATSDSSVITYT 306 Query: 116 *INSV 102 INSV Sbjct: 307 LINSV 311 >SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizosaccharomyces pombe|chr 3|||Manual Length = 1045 Score = 25.4 bits (53), Expect = 9.5 Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 1/47 (2%) Frame = +2 Query: 503 NSLQEVRRLMTLKDNMKLSQPPEGSTF*FLQSSLNRPNL-LSHKTII 640 N ++RRL L + +Q PE S F +Q SL+ NL LS+ T + Sbjct: 813 NRPPDIRRLKRLVNFSFRTQDPEASNF-VIQPSLDIRNLYLSNNTFV 858 >SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 718 Score = 25.4 bits (53), Expect = 9.5 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +1 Query: 187 RNFRLLEELEQGQKGVGDGTISW 255 R+ R+LE LEQ +G+ GT+ + Sbjct: 628 RSVRMLEPLEQHGRGIYSGTLGY 650 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,335,116 Number of Sequences: 5004 Number of extensions: 70040 Number of successful extensions: 168 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 390427050 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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