BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120354.Seq (636 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g01640.1 68417.m00213 hypothetical protein 30 1.5 At2g34280.1 68415.m04194 S locus F-box-related / SLF-related con... 30 1.5 At5g19720.1 68418.m02345 tRNA synthetase class I (E and Q) famil... 28 4.5 At2g45270.1 68415.m05635 glycoprotease M22 family protein simila... 28 6.0 At2g19190.1 68415.m02239 light-responsive receptor protein kinas... 28 6.0 >At4g01640.1 68417.m00213 hypothetical protein Length = 300 Score = 29.9 bits (64), Expect = 1.5 Identities = 18/45 (40%), Positives = 22/45 (48%) Frame = -2 Query: 368 HSVRELALHN*LDNTLLGDEHCVRLQVQWVTLLDHVVWAGVYGID 234 +S REL + N LD+ L E QV W DH W +Y ID Sbjct: 185 YSSRELIMCN-LDDRLCVSEKMWPNQVIWSLDSDHKTWKEIYSID 228 >At2g34280.1 68415.m04194 S locus F-box-related / SLF-related contains Pfam PF00646: F-box domain; contains weak hit to TIGRFAM TIGR01640 : F-box protein interaction domain; weakly similar to self-incompatibility (S-) locus F-box (GI:29420811) [Prunus mume] Length = 391 Score = 29.9 bits (64), Expect = 1.5 Identities = 18/45 (40%), Positives = 22/45 (48%) Frame = -2 Query: 368 HSVRELALHN*LDNTLLGDEHCVRLQVQWVTLLDHVVWAGVYGID 234 +S REL + N LD+ L E QV W DH W +Y ID Sbjct: 276 YSSRELIMCN-LDDRLCVSEKMWPNQVIWSLDSDHKTWKEIYSID 319 >At5g19720.1 68418.m02345 tRNA synthetase class I (E and Q) family protein similar to tRNA-glutamine synthetase [Lupinus luteus] GI:2995455; contains Pfam profile PF03950: tRNA synthetases class I (E and Q), anti-codon binding domain Length = 170 Score = 28.3 bits (60), Expect = 4.5 Identities = 14/46 (30%), Positives = 24/46 (52%) Frame = -2 Query: 323 LLGDEHCVRLQVQWVTLLDHVVWAGVYGIDPDSLYVVLYFLSTLTI 186 L E+ L W+T ++ + +G Y +D DS+ L F T+T+ Sbjct: 116 LFNSENPAELNDDWLTDINPQLQSGYYTVDKDSIPGKLVFNRTVTL 161 >At2g45270.1 68415.m05635 glycoprotease M22 family protein similar to SP|P36175 O-sialoglycoprotein endopeptidase (EC 3.4.24.57) (Glycoprotease) {Pasteurella haemolytica}; contains Pfam profile PF00814: Glycoprotease family Length = 480 Score = 27.9 bits (59), Expect = 6.0 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +3 Query: 237 YTIYPGPDNVVKQGDPLYLQPHTVLITKEGVIQLIMKSKLP 359 + +YPG + + + L LQ H L TK LI S P Sbjct: 15 FNLYPGISILARNNNSLRLQKHHKLKTKTPTFSLISPSSSP 55 >At2g19190.1 68415.m02239 light-responsive receptor protein kinase / senescence-responsive receptor-like serine/threonine kinase, putative (SIRK) similar to light repressible receptor protein kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376; contains Pfam profiles PF00069: Protein kinase domain and PF00560: Leucine Rich Repeat Length = 876 Score = 27.9 bits (59), Expect = 6.0 Identities = 22/91 (24%), Positives = 43/91 (47%), Gaps = 4/91 (4%) Frame = +3 Query: 210 QNDVQRVGVY---TIYPGPDNVVKQGDPLYLQPHTVLITKEGVIQLIMKSKLPYAVEL-Q 377 ++DV +GV I P + + +++ H I G I+ I+ +L ++ Sbjct: 754 KSDVYSLGVVLLEVITGQPAIASSKTEKVHISDHVRSILANGDIRGIVDQRLRERYDVGS 813 Query: 378 AWLLEEVIPQCCARASTRRPSRWTQIMV*LK 470 AW + E+ C S +RP+ +Q+++ LK Sbjct: 814 AWKMSEIALACTEHTSAQRPT-MSQVVMELK 843 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,545,667 Number of Sequences: 28952 Number of extensions: 283130 Number of successful extensions: 630 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 619 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 630 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1305036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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