BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120321.Seq (814 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_31183| Best HMM Match : No HMM Matches (HMM E-Value=.) 55 8e-08 SB_57825| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.9 SB_27940| Best HMM Match : IncA (HMM E-Value=0.41) 30 1.9 SB_15804| Best HMM Match : Toxin_28 (HMM E-Value=8.6) 30 1.9 SB_10369| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.9 SB_5747| Best HMM Match : NAF1 (HMM E-Value=7.3) 30 1.9 SB_16260| Best HMM Match : Ribosomal_L12 (HMM E-Value=1.4) 30 1.9 SB_13929| Best HMM Match : VKOR (HMM E-Value=0.65) 30 1.9 SB_11550| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.9 SB_10191| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.9 SB_42842| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.6 SB_38773| Best HMM Match : Flagellin_IN (HMM E-Value=9.9) 30 2.6 SB_8210| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.6 SB_37766| Best HMM Match : IncA (HMM E-Value=0.4) 30 2.6 SB_10025| Best HMM Match : GLYCAM-1 (HMM E-Value=1.3) 30 2.6 SB_6351| Best HMM Match : IncA (HMM E-Value=0.13) 30 2.6 SB_22296| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.4 SB_45530| Best HMM Match : DnaB_C (HMM E-Value=0.89) 29 3.4 SB_59750| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.5 SB_45711| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.5 SB_19157| Best HMM Match : FLO_LFY (HMM E-Value=0.27) 29 4.5 SB_2785| Best HMM Match : ATP-synt_F (HMM E-Value=0.15) 29 5.9 SB_20653| Best HMM Match : Sialidase (HMM E-Value=0.35) 29 5.9 SB_11154| Best HMM Match : Flagellin_IN (HMM E-Value=2.2) 29 5.9 SB_58552| Best HMM Match : UbiD (HMM E-Value=1.1) 28 7.8 SB_57351| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.8 SB_50121| Best HMM Match : DoxX (HMM E-Value=1.6) 28 7.8 SB_42687| Best HMM Match : UPF0262 (HMM E-Value=2.1) 28 7.8 SB_40685| Best HMM Match : NPIP (HMM E-Value=1.8) 28 7.8 SB_29421| Best HMM Match : N_methyl (HMM E-Value=0.58) 28 7.8 SB_18689| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.8 SB_59013| Best HMM Match : DoxX (HMM E-Value=1.6) 28 7.8 SB_34369| Best HMM Match : Herpes_US9 (HMM E-Value=0.64) 28 7.8 >SB_31183| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 128 Score = 54.8 bits (126), Expect = 8e-08 Identities = 23/34 (67%), Positives = 26/34 (76%) Frame = +2 Query: 257 YSDANKAKGITWNDDTLFEYLENPKKYIPGTKMV 358 Y+ AN+ KGITW +DTL YLENPKKYIPG V Sbjct: 74 YTTANQNKGITWGEDTLMVYLENPKKYIPGRSNV 107 Score = 52.4 bits (120), Expect = 4e-07 Identities = 21/24 (87%), Positives = 21/24 (87%) Frame = +3 Query: 183 GGKHKVGPNLHGFFGRKTGQAAGF 254 GGKHK GPNLHG FGRKTGQA GF Sbjct: 49 GGKHKTGPNLHGLFGRKTGQAEGF 72 >SB_57825| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 495 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_27940| Best HMM Match : IncA (HMM E-Value=0.41) Length = 418 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_15804| Best HMM Match : Toxin_28 (HMM E-Value=8.6) Length = 329 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_10369| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 386 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_5747| Best HMM Match : NAF1 (HMM E-Value=7.3) Length = 268 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_16260| Best HMM Match : Ribosomal_L12 (HMM E-Value=1.4) Length = 341 Score = 30.3 bits (65), Expect = 1.9 Identities = 22/90 (24%), Positives = 43/90 (47%), Gaps = 3/90 (3%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGIYFL-FSPIS 506 R+ SL WY L+ Q++ +++LP LP+ E+ + +I S I P++ Sbjct: 30 RHCSLFNSWYNLEREHQITTISVLPPIATLPAGHHTAESIVETINRTESKILTAKLDPLT 89 Query: 507 --ISVMYSAMLQLHYNIKVFVSSKSQHNHL 590 I++M S + L+ + +++ N L Sbjct: 90 WKIALMSSGVAFLNAELCALFGLEAKDNQL 119 >SB_13929| Best HMM Match : VKOR (HMM E-Value=0.65) Length = 498 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 394 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 444 >SB_11550| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 418 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_10191| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 340 Score = 30.3 bits (65), Expect = 1.9 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ ++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTISSLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_42842| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 385 Score = 29.9 bits (64), Expect = 2.6 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ + LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTIASLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_38773| Best HMM Match : Flagellin_IN (HMM E-Value=9.9) Length = 135 Score = 29.9 bits (64), Expect = 2.6 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ + LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTIASLPPIATLPAGHHTAESIVETINHAKSQI 80 >SB_8210| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1935 Score = 29.9 bits (64), Expect = 2.6 Identities = 25/87 (28%), Positives = 38/87 (43%) Frame = +3 Query: 189 KHKVGPNLHGFFGRKTGQAAGFHTPMPIKLRALHGMTTLSLNILRIPRNTSLEPRWYLLD 368 K GPN F G+K G + P+P K+ + LS ++ + L WY D Sbjct: 165 KLSTGPNFITFLGQKYG-----YRPLPHKIDSREFEKLLS--VITSTEDLDLVKYWYWRD 217 Query: 369 SRRQMSVLTLLPISNLLPSNFKVIENS 449 L PIS+ LP +F+ E++ Sbjct: 218 DNNVPPEYILQPISSRLP-HFRDAEHA 243 >SB_37766| Best HMM Match : IncA (HMM E-Value=0.4) Length = 585 Score = 29.9 bits (64), Expect = 2.6 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ + LP LP+ E+ + +I H S I Sbjct: 275 RQCSLFNSWYNLEREQQITTIASLPPIATLPAGHHTAESIVETINHAKSQI 325 >SB_10025| Best HMM Match : GLYCAM-1 (HMM E-Value=1.3) Length = 517 Score = 29.9 bits (64), Expect = 2.6 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ + LP LP+ E+ + +I H S I Sbjct: 322 RQCSLFNSWYNLEREQQITTIASLPPIATLPAGHHTAESIVETINHAKSQI 372 >SB_6351| Best HMM Match : IncA (HMM E-Value=0.13) Length = 417 Score = 29.9 bits (64), Expect = 2.6 Identities = 14/46 (30%), Positives = 23/46 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFH 467 R SL WY L+ Q++ + LP LP+ E+++ +I H Sbjct: 30 RQCSLFNSWYNLEREHQITTIASLPPIVTLPAGHHTAESTVETINH 75 >SB_22296| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 681 Score = 29.5 bits (63), Expect = 3.4 Identities = 22/83 (26%), Positives = 38/83 (45%), Gaps = 3/83 (3%) Frame = +3 Query: 222 FGRKTGQAAGFHTPMPIKLRALHGMTTL---SLNILRIPRNTSLEPRWYLLDSRRQMSVL 392 FGR+ + A H P+ + H + T+ + +I SL WY L+ Q++ + Sbjct: 249 FGRR--KPAKLHAPVVVVAYETHLLPTIVRPTFVAKQIEPRVSLAA-WYNLEREHQITTI 305 Query: 393 TLLPISNLLPSNFKVIENSIRSI 461 + LP LPS E+ + +I Sbjct: 306 SALPPIATLPSGHHTAESIVETI 328 >SB_45530| Best HMM Match : DnaB_C (HMM E-Value=0.89) Length = 396 Score = 29.5 bits (63), Expect = 3.4 Identities = 14/44 (31%), Positives = 23/44 (52%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSI 461 R+ SL WY L+ Q++ ++ LP LPS E+ + +I Sbjct: 30 RDCSLFNSWYNLEREHQITTISALPPIATLPSGHHTAESIVETI 73 >SB_59750| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 607 Score = 29.1 bits (62), Expect = 4.5 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ + LP LP+ E+ + +I H S I Sbjct: 297 RQCSLFNSWYNLEREQQITTIASLPPIATLPAGNHTAESIVETINHAKSQI 347 >SB_45711| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 178 Score = 29.1 bits (62), Expect = 4.5 Identities = 15/33 (45%), Positives = 20/33 (60%) Frame = -1 Query: 367 SSKYHLGSRDVFLGILKIFKESVVIPCNALSFI 269 +S Y L + +F G L+ K SVV+P LSFI Sbjct: 91 ASFYSLATLGMFAGFLRTKKPSVVVPFLPLSFI 123 >SB_19157| Best HMM Match : FLO_LFY (HMM E-Value=0.27) Length = 560 Score = 29.1 bits (62), Expect = 4.5 Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 1/52 (1%) Frame = +3 Query: 330 RNTSLEP-RWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R +L P RWY L+ +Q++ LP LP+ E+ + +I H S I Sbjct: 296 RRGALPPGRWYNLEREQQITTTDSLPTIATLPAGHHTAESIMETINHAKSQI 347 >SB_2785| Best HMM Match : ATP-synt_F (HMM E-Value=0.15) Length = 379 Score = 28.7 bits (61), Expect = 5.9 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ + LP LP+ E + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTIAPLPPIATLPAGHNTAEAIVETINHAKSQI 80 >SB_20653| Best HMM Match : Sialidase (HMM E-Value=0.35) Length = 572 Score = 28.7 bits (61), Expect = 5.9 Identities = 20/95 (21%), Positives = 45/95 (47%), Gaps = 3/95 (3%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGIYFL-FSPIS 506 R SL WY L+ Q++++ LP LP+ E+ + +I S I P++ Sbjct: 30 RQCSLFNSWYNLEREHQITIIASLPPIATLPAGHHTAESIVETINRAESKILTAKLDPLT 89 Query: 507 --ISVMYSAMLQLHYNIKVFVSSKSQHNHLQLLFV 605 I+++ + L+ + +++++ L+ L++ Sbjct: 90 GKIALVSKGVAFLNAELCALFGLEAKNDQLEALYI 124 >SB_11154| Best HMM Match : Flagellin_IN (HMM E-Value=2.2) Length = 311 Score = 28.7 bits (61), Expect = 5.9 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEQEQQITTAASLPPIVTLPAGHHTAESIVETINHAKSQI 80 >SB_58552| Best HMM Match : UbiD (HMM E-Value=1.1) Length = 409 Score = 28.3 bits (60), Expect = 7.8 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ LP LP+ E+ + +I H S I Sbjct: 30 RQCSLFNSWYNLEREQQITTTDSLPTIATLPAGHHTAESIMETINHAKSQI 80 >SB_57351| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 559 Score = 28.3 bits (60), Expect = 7.8 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY L+ +Q++ LP LP+ E+ + +I H S I Sbjct: 373 RQCSLFNSWYNLEREQQITTTDSLPTIATLPAGHHTAESIMETINHAKSQI 423 >SB_50121| Best HMM Match : DoxX (HMM E-Value=1.6) Length = 338 Score = 28.3 bits (60), Expect = 7.8 Identities = 15/51 (29%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY ++ +Q++ LP LP+ + E+ + +I H S I Sbjct: 30 RQCSLFNSWYNVEREQQITTTDSLPTIATLPAGHRTTESIMETINHAKSQI 80 >SB_42687| Best HMM Match : UPF0262 (HMM E-Value=2.1) Length = 908 Score = 28.3 bits (60), Expect = 7.8 Identities = 13/44 (29%), Positives = 23/44 (52%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSI 461 R SL WY L+ Q++ ++ LP LP+ + E+ + +I Sbjct: 794 RQCSLFNSWYNLEREHQITTISALPPIATLPAGHHMAESIVETI 837 >SB_40685| Best HMM Match : NPIP (HMM E-Value=1.8) Length = 672 Score = 28.3 bits (60), Expect = 7.8 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSI 461 R SL WY L+ Q++ ++ LP LP+ E+ + +I Sbjct: 30 RQCSLFNSWYNLEQEHQITTISALPPIATLPAGHHTAESIVETI 73 >SB_29421| Best HMM Match : N_methyl (HMM E-Value=0.58) Length = 534 Score = 28.3 bits (60), Expect = 7.8 Identities = 13/44 (29%), Positives = 23/44 (52%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSI 461 R+ SL WY L+ Q++ ++ LP LP+ E+ + +I Sbjct: 323 RHCSLFNSWYNLEREHQITTISALPPIATLPAGHHTAESIVETI 366 >SB_18689| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 160 Score = 28.3 bits (60), Expect = 7.8 Identities = 15/36 (41%), Positives = 22/36 (61%) Frame = -2 Query: 813 LVYLQFNNLKLVTL*DLNKLMKFIANDNFFVKRLSQ 706 L YL+ N+ + VTL +L +++K A D F KR Q Sbjct: 33 LEYLEKNDDETVTLDELLEILKHKAGDGVFAKRTLQ 68 >SB_59013| Best HMM Match : DoxX (HMM E-Value=1.6) Length = 338 Score = 28.3 bits (60), Expect = 7.8 Identities = 15/51 (29%), Positives = 25/51 (49%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSIFHNISGI 482 R SL WY ++ +Q++ LP LP+ + E+ + +I H S I Sbjct: 30 RQCSLFNSWYNVEREQQITTTDSLPTIATLPAGHRTTESIMETINHAKSQI 80 >SB_34369| Best HMM Match : Herpes_US9 (HMM E-Value=0.64) Length = 361 Score = 28.3 bits (60), Expect = 7.8 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +3 Query: 330 RNTSLEPRWYLLDSRRQMSVLTLLPISNLLPSNFKVIENSIRSI 461 R SL WY L+ Q++ + LP LP+ + E+ + +I Sbjct: 80 RQCSLFNSWYNLEREHQITTIAALPPIATLPAGHQTAESIVETI 123 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 25,214,718 Number of Sequences: 59808 Number of extensions: 554728 Number of successful extensions: 1431 Number of sequences better than 10.0: 33 Number of HSP's better than 10.0 without gapping: 1319 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1428 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2263654701 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -