BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120305.Seq (722 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 122 4e-29 SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 111 1e-25 SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 47 2e-06 SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.17 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.22 SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 30 0.29 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.2 SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 27 3.6 SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.6 SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 27 3.6 SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 4.7 SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 26 4.7 SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 8.3 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 8.3 >SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 122 bits (295), Expect = 4e-29 Identities = 62/81 (76%), Positives = 72/81 (88%) Frame = +1 Query: 256 RPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLA 435 RP+LI+K+ IPVVMAGIIAIYGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLA Sbjct: 47 RPDLIVKNTIPVVMAGIIAIYGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLA 104 Query: 436 AGFAIGIVGDAGVRGTAQQPR 498 AGFAIGIVGDAGVRGTAQQPR Sbjct: 105 AGFAIGIVGDAGVRGTAQQPR 125 Score = 62.5 bits (145), Expect = 6e-11 Identities = 25/37 (67%), Positives = 32/37 (86%) Frame = +2 Query: 137 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAM 247 P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAM 43 Score = 41.1 bits (92), Expect = 2e-04 Identities = 20/22 (90%), Positives = 21/22 (95%) Frame = +3 Query: 510 MILILIFAEVLGLYGLIVAIYL 575 MILILIFAEVLGLYGLIVA+ L Sbjct: 130 MILILIFAEVLGLYGLIVALLL 151 >SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 111 bits (266), Expect = 1e-25 Identities = 50/81 (61%), Positives = 66/81 (81%) Frame = +1 Query: 256 RPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLA 435 RPE++MKS+IPVVM+GII +YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+A Sbjct: 47 RPEIVMKSLIPVVMSGIIGVYGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVA 106 Query: 436 AGFAIGIVGDAGVRGTAQQPR 498 AG+AIG+VGD GV+ +Q R Sbjct: 107 AGYAIGVVGDRGVQSFMRQDR 127 Score = 50.0 bits (114), Expect = 3e-07 Identities = 21/37 (56%), Positives = 27/37 (72%) Frame = +2 Query: 137 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAM 247 PIY FFG G ++++FS LGA YGTA +G GIAA+ Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAV 43 Score = 39.1 bits (87), Expect = 6e-04 Identities = 18/22 (81%), Positives = 20/22 (90%) Frame = +3 Query: 510 MILILIFAEVLGLYGLIVAIYL 575 M+LILIFAEVLGLYGLIV + L Sbjct: 132 MVLILIFAEVLGLYGLIVGLIL 153 >SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 47.2 bits (107), Expect = 2e-06 Identities = 23/83 (27%), Positives = 41/83 (49%), Gaps = 6/83 (7%) Frame = +1 Query: 259 PELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQE--PANY----PLYKGFIHLGAGLAVG 420 P + K++I ++ ++AIY L++A++ + + + PA + Y GF G+ VG Sbjct: 80 PRIKTKNLISIIFCEVVAIYSLIIAIVFSAKINDINPAGFYTKSHYYTGFALFWGGITVG 139 Query: 421 FSGLAAGFAIGIVGDAGVRGTAQ 489 L G +GI G + AQ Sbjct: 140 LCNLICGVCVGITGSSAALADAQ 162 Score = 29.1 bits (62), Expect = 0.67 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = +2 Query: 155 FGVMGAASAIIFSALGAAYGTAKSGTGI 238 +G++G AS + F +GAA+G GT I Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSI 72 Score = 27.5 bits (58), Expect = 2.1 Identities = 10/23 (43%), Positives = 18/23 (78%) Frame = +3 Query: 507 RMILILIFAEVLGLYGLIVAIYL 575 +++++ IF VLGL+GLIV + + Sbjct: 169 KVLVVEIFGSVLGLFGLIVGLLI 191 >SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 288 Score = 31.1 bits (67), Expect = 0.17 Identities = 21/68 (30%), Positives = 30/68 (44%) Frame = +1 Query: 280 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 459 II +AG+IA + +++ IAG + G ++ G L LA GF I Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246 Query: 460 GDAGVRGT 483 GD V T Sbjct: 247 GDVCVSNT 254 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 30.7 bits (66), Expect = 0.22 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = -1 Query: 485 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 384 +VP P++P MP P+A P A AP NP Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2|Schizosaccharomyces pombe|chr 2|||Manual Length = 659 Score = 30.3 bits (65), Expect = 0.29 Identities = 24/78 (30%), Positives = 36/78 (46%), Gaps = 5/78 (6%) Frame = -1 Query: 575 QVDGDDKSVKTQYFSENKNKNHSTNNLGC*AVPR-----TPASPTMPMAKPAARPENPTA 411 Q +G++KS K FSE+ + NH + + P +P SPT ++ E T Sbjct: 329 QSNGNEKSSKYNVFSESAHGNHQVLSFSPGSSPSFIEQPSPISPTSTTSEDTNTLEEDTD 388 Query: 410 KPAPKWMNPL*RG*LAGS 357 + KW+ RG L GS Sbjct: 389 DQSIKWI----RGALIGS 402 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 28.3 bits (60), Expect = 1.2 Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%) Frame = -1 Query: 485 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 312 A P P S P++PM PAA P P+A AP PL AG AP + A P Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478 Query: 311 AIIPA 297 A PA Sbjct: 479 APAPA 483 >SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating transcription Rct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 26.6 bits (56), Expect = 3.6 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -1 Query: 95 TGPKNCDDYLHTLTPRNYFCTDK 27 TG KNCDD+L +P + D+ Sbjct: 402 TGNKNCDDHLRDKSPERRYRYDR 424 >SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 281 Score = 26.6 bits (56), Expect = 3.6 Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 3/31 (9%) Frame = -1 Query: 581 CVQVDGDD-KSV--KTQYFSENKNKNHSTNN 498 C++VD +D K + K+QY +EN N N S NN Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTN-SINN 262 >SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 447 Score = 26.6 bits (56), Expect = 3.6 Identities = 16/59 (27%), Positives = 32/59 (54%), Gaps = 3/59 (5%) Frame = -1 Query: 584 FCVQVDGDDKSVKTQYFSENKNKNHSTNNLGC*--AVPRT-PASPTMPMAKPAARPENP 417 F Q+ + + + + SE++ ++ TN+ G ++P++ P SP M P++ P NP Sbjct: 126 FNPQLPSNSNTNRFDFGSESQLSSNYTNDTGLSLLSLPKSVPHSPAMHKRTPSSDPNNP 184 >SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces pombe|chr 2|||Manual Length = 760 Score = 26.2 bits (55), Expect = 4.7 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = -3 Query: 393 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 280 DEPF+K +L+ SN++S+ VD N+ D +D Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85 >SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 629 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = +1 Query: 439 GFAIGIVGDAGVRGTAQQPRXXXXXXXXXXXXKYWVFTDLSSPS 570 G A+G G+ G R +++P Y+ F DLS P+ Sbjct: 520 GSAVGQNGNVGPRQISEEPMSIIFNLGISNNWAYYYFRDLSFPA 563 >SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 25.4 bits (53), Expect = 8.3 Identities = 8/13 (61%), Positives = 10/13 (76%) Frame = -1 Query: 95 TGPKNCDDYLHTL 57 TGP N DDY+H + Sbjct: 369 TGPSNTDDYIHRI 381 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%) Frame = -1 Query: 485 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 396 +VP+ PA+P +P A +P A P P AP+ Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,206,695 Number of Sequences: 5004 Number of extensions: 67760 Number of successful extensions: 228 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 208 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 227 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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