BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120295.Seq (716 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 29 0.88 SPBC582.03 |cdc13||cyclin Cdc13|Schizosaccharomyces pombe|chr 2|... 28 1.5 SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 28 1.5 SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 27 2.0 SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 26 4.7 SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 26 4.7 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 6.2 SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual 26 6.2 SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 6.2 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 25 8.2 SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 25 8.2 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 8.2 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 28.7 bits (61), Expect = 0.88 Identities = 18/81 (22%), Positives = 35/81 (43%) Frame = -3 Query: 705 KNSIYYALRKHFAGHPMLKRLTTYKNF*LPLRKLFMRAICRSVIVEPITFIXXXXXXXXS 526 ++ ++Y + P+L+ LT + N +P +L A+C ++ S Sbjct: 512 ESEVFYYFENYDEDIPILRNLTEFYNIAIPWLRLSGLALCHDPVIVTKLCRIGQKCVDNS 571 Query: 525 NVMRLCWL*TCCAVWLPLLCL 463 + R WL ++ LPL+ L Sbjct: 572 SESRTLWLDIIRSLLLPLITL 592 >SPBC582.03 |cdc13||cyclin Cdc13|Schizosaccharomyces pombe|chr 2|||Manual Length = 482 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = +1 Query: 469 K*RKPYRATSSKPTQPHHVAGRLHARGANERDRLHYDRTT 588 K R+P SS P+ P H++ + H+ + D H D+ T Sbjct: 113 KRRQPSVFNSSVPSLPQHLSTKSHSVSTHGVDAFHKDQAT 152 >SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1727 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/47 (25%), Positives = 25/47 (53%) Frame = +2 Query: 467 QSKGSHTAQQVQSQHNRITLLEDYTREELMNVIGSTMTERQIARMNS 607 Q++ SH +QVQS H ++ +E+ ++ + S + E + + S Sbjct: 153 QNENSHLGEQVQSAHQALSDIEERKKQHMFASSSSRVKEEILVQEKS 199 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 27.5 bits (58), Expect = 2.0 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = +1 Query: 82 DVIAKIDDLTQKLTVANAD 138 D +KID LT+KL VANA+ Sbjct: 618 DSASKIDSLTEKLKVANAE 636 >SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2176 Score = 26.2 bits (55), Expect = 4.7 Identities = 20/54 (37%), Positives = 24/54 (44%), Gaps = 2/54 (3%) Frame = -2 Query: 325 RLWHSCGLLGLAITSCAMSAMRL--AAAPFRGARFRNLAEPFQRRVEPQSFRWQ 170 R W S L L +C M RL +P R +F N RRVE + F WQ Sbjct: 1140 RGWSSVATLSL--DTCKMIEKRLWPTMSPLR--QFPNCPSEVIRRVEKKEFPWQ 1189 >SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 437 Score = 26.2 bits (55), Expect = 4.7 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%) Frame = -1 Query: 164 MSDRLASAKSALATVNFCVKSSILAI-----TSFVSISTAGAYLPVHST*GMT 21 + DRL SA+S T C+ ++ LA T +VS T G + V S G+T Sbjct: 128 IKDRLVSAQSISGTGALCIAANFLASFYPSKTIYVSDPTWGNHKNVFSRAGLT 180 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 25.8 bits (54), Expect = 6.2 Identities = 12/47 (25%), Positives = 25/47 (53%) Frame = +3 Query: 210 GSARLRKRAPRNGAAANRMADIAQDVIAKPSNPQLCHSLAVCDVGNN 350 G +++ KRA + +AD+++ ++ S HS A+ ++G N Sbjct: 447 GGSQIEKRANESNNLQREIADLSEQIVELESKRNDLHS-ALLEMGGN 492 >SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual Length = 264 Score = 25.8 bits (54), Expect = 6.2 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = +1 Query: 364 CARKNDRWAAA*NDW 408 C RK++RW AA N W Sbjct: 140 CRRKDNRWFAAINKW 154 >SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 25.8 bits (54), Expect = 6.2 Identities = 18/62 (29%), Positives = 27/62 (43%) Frame = +1 Query: 52 YAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCEN 231 YAP+V + I L + + A LA SL+LF + V +D ET + + Sbjct: 146 YAPSVAKANEGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLEVIEQDEETGDVEMSS 205 Query: 232 AR 237 R Sbjct: 206 MR 207 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 25.4 bits (53), Expect = 8.2 Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 4/44 (9%) Frame = +1 Query: 136 DLAEANRSLILF----ANEMIVARRDAETARQDCENARRETAQL 255 DL +ANR L ANE + +RD R+D + +R+ QL Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQL 361 >SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 471 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/26 (50%), Positives = 16/26 (61%), Gaps = 4/26 (15%) Frame = -1 Query: 533 LPAT*CGCVGFE----LVARYGFLYF 468 LPA GCVGF+ ++RYG L F Sbjct: 168 LPAAYVGCVGFKPSFGRISRYGMLAF 193 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 8.2 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +1 Query: 166 LFANEMIVARRDAETARQDCE 228 +FANE+++ + DA++ +QD E Sbjct: 556 IFANEILIEQADAQSFKQDEE 576 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,839,308 Number of Sequences: 5004 Number of extensions: 53578 Number of successful extensions: 137 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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