Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120295.Seq
(716 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74680.1 68414.m08648 exostosin family protein contains Pfam ... 31 1.0
At3g24660.1 68416.m03096 leucine-rich repeat transmembrane prote... 30 1.3
At1g74360.1 68414.m08615 leucine-rich repeat transmembrane prote... 30 1.3
At1g09970.2 68414.m01124 leucine-rich repeat transmembrane prote... 30 1.8
At1g09970.1 68414.m01123 leucine-rich repeat transmembrane prote... 30 1.8
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 25 4.6
At1g33880.1 68414.m04200 avirulence-responsive family protein / ... 28 5.4
At5g04730.1 68418.m00484 expressed protein ; expression supporte... 28 7.1
At3g13380.1 68416.m01683 leucine-rich repeat family protein / pr... 27 9.4
At3g09370.1 68416.m01111 myb family transcription factor (MYB3R3... 27 9.4
At2g20450.1 68415.m02387 60S ribosomal protein L14 (RPL14A) 27 9.4
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 27 9.4
At1g17230.1 68414.m02099 leucine-rich repeat family protein / pr... 27 9.4
At1g05460.1 68414.m00555 RNA helicase SDE3 (SDE3) identical to R... 27 9.4
>At1g74680.1 68414.m08648 exostosin family protein contains Pfam
profile: PF03016 Exostosin family
Length = 461
Score = 30.7 bits (66), Expect = 1.0
Identities = 19/78 (24%), Positives = 39/78 (50%)
Frame = +1
Query: 46 GKYAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDC 225
G+Y+ A+ D+IA + + T++N + A + +L + + R+D T RQ+
Sbjct: 237 GRYSSAIANLEKDIIAPYVHVVK--TISNNESASFEKRPVLAYFQGAIYRKDGGTIRQEL 294
Query: 226 ENARRETAQLPTAWRTLR 279
N ++ + A+ T+R
Sbjct: 295 YNLLKDEKDVHFAFGTVR 312
>At3g24660.1 68416.m03096 leucine-rich repeat transmembrane protein
kinase, putative identical to putative kinase-like
protein TMKL1 precursor GB:P33543 from [Arabidopsis
thaliana], (Plant Mol. Biol. 23 (2), 415-421 (1993))
Length = 674
Score = 30.3 bits (65), Expect = 1.3
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = +3
Query: 285 VIAKPSNPQ-LCHSLAVCDVGNNEFAFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSMNV 461
V+ +P+ P C +L V D+G N+F+ P+ + + +K L + +F VP + V
Sbjct: 187 VLPEPALPNSTCGNLQVLDLGGNKFSGEFPEFITRFKGVKSLDLSSNVFEG-LVPEGLGV 245
Query: 462 LNKVKEAIPRNKF 500
L + N F
Sbjct: 246 LELESLNLSHNNF 258
>At1g74360.1 68414.m08615 leucine-rich repeat transmembrane protein
kinase, putative similar to brassinosteroid insensitive
1 GB:AAC49810 (putative receptor protein kinase);
contains Pfam profiles: PF00560 Leucine Rich Repeat (17
repeats), PF00069 Eukaryotic protein kinase domain
Length = 1106
Score = 30.3 bits (65), Expect = 1.3
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 297 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSMNVLNKVK 476
P C +L V ++ N+F P + SLK L + FS D +N+ N V
Sbjct: 269 PGQVSNCQNLNVLNLWGNKFTGNIPAEIGSISSLKGLYLGNNTFSRDIPETLLNLTNLVF 328
Query: 477 EAIPRNKF 500
+ RNKF
Sbjct: 329 LDLSRNKF 336
>At1g09970.2 68414.m01124 leucine-rich repeat transmembrane protein
kinase, putative Similar to A. thaliana receptor-like
protein kinase (gb|RLK5_ARATH). ESTs
gb|ATTS0475,gb|ATTS4362 come from this gene isoform
contains a TG acceptor site at intron.
Length = 977
Score = 29.9 bits (64), Expect = 1.8
Identities = 24/71 (33%), Positives = 37/71 (52%), Gaps = 1/71 (1%)
Frame = +3
Query: 297 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSM-NVLNKV 473
PS+ + C SL D+GNN F+ P+ SL + L+ L N+ FS + S+ N + V
Sbjct: 114 PSDLKNCTSLKYLDLGNNLFSGAFPEFSSLNQ-LQFLYLNNSAFSGVFPWKSLRNATSLV 172
Query: 474 KEAIPRNKFKA 506
++ N F A
Sbjct: 173 VLSLGDNPFDA 183
>At1g09970.1 68414.m01123 leucine-rich repeat transmembrane protein
kinase, putative Similar to A. thaliana receptor-like
protein kinase (gb|RLK5_ARATH). ESTs
gb|ATTS0475,gb|ATTS4362 come from this gene isoform
contains a TG acceptor site at intron.
Length = 976
Score = 29.9 bits (64), Expect = 1.8
Identities = 24/71 (33%), Positives = 37/71 (52%), Gaps = 1/71 (1%)
Frame = +3
Query: 297 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSM-NVLNKV 473
PS+ + C SL D+GNN F+ P+ SL + L+ L N+ FS + S+ N + V
Sbjct: 114 PSDLKNCTSLKYLDLGNNLFSGAFPEFSSLNQ-LQFLYLNNSAFSGVFPWKSLRNATSLV 172
Query: 474 KEAIPRNKFKA 506
++ N F A
Sbjct: 173 VLSLGDNPFDA 183
>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
similar to GTL1 [Arabidopsis thaliana] GI:2664198
Length = 669
Score = 25.4 bits (53), Expect(2) = 4.6
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = -1
Query: 269 RHAVGSCAVSRRAFSQSCRAVSASRRATIISLANKMSDRLASAKSALAT 123
R + A SQ RA SASR A IISL K++ +L++
Sbjct: 308 RQEMARLAREHEVMSQE-RAASASRDAAIISLIQKITGHTIQLPPSLSS 355
Score = 21.4 bits (43), Expect(2) = 4.6
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -1
Query: 398 QAAAQRSFLRAQKRK 354
QAA QRSFL A +++
Sbjct: 281 QAAMQRSFLEALEKR 295
>At1g33880.1 68414.m04200 avirulence-responsive family protein /
avirulence induced gene (AIG1) family protein similar to
AIG1 protein SP:P54120 [Arabidopsis thaliana]; contains
Pfam PF04548: AIG1 family;
Length = 234
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = +2
Query: 473 KGSHTAQQVQSQHNRITLLEDYTREELMNVIGSTMTE 583
+G H V S NRIT E+Y L + GS + E
Sbjct: 102 EGVHAVLFVLSMKNRITQEEEYALNTLQRIFGSKILE 138
>At5g04730.1 68418.m00484 expressed protein ; expression supported
by MPSS
Length = 321
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/51 (27%), Positives = 27/51 (52%)
Frame = +2
Query: 503 SQHNRITLLEDYTREELMNVIGSTMTERQIARMNSLRNGN*KFLYVVKRLS 655
S N L ++ +E++ N+I + +R++ + S GN L++ RLS
Sbjct: 46 SGRNLFQLAVEFKKEKIFNLIHG-LDDRKVTLLRSYDKGNNNILHIAGRLS 95
>At3g13380.1 68416.m01683 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1164
Score = 27.5 bits (58), Expect = 9.4
Identities = 18/76 (23%), Positives = 32/76 (42%), Gaps = 1/76 (1%)
Frame = +3
Query: 297 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSMNVLNKVK 476
P LC +L V D+ N PQ + SL+ L + S D++ ++ L+++
Sbjct: 295 PELSLLCRTLEVLDLSGNSLTGQLPQSFTSCGSLQSLNLGNNKLSGDFLSTVVSKLSRIT 354
Query: 477 EA-IPRNKFKANTTAS 521
+P N + S
Sbjct: 355 NLYLPFNNISGSVPIS 370
>At3g09370.1 68416.m01111 myb family transcription factor (MYB3R3)
contains Pfam profile: Myb DNA-binding proteins;
identical to cDNA putative c-myb-like transcription
factor (MYB3R3) GI:15375285
Length = 505
Score = 27.5 bits (58), Expect = 9.4
Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = -2
Query: 265 MRLAAAPFRGARFRNLAEPFQRRVEPQSF-RWQ 170
+R A F+G ++N+A+ F R E Q RWQ
Sbjct: 89 LRQAVDTFKGKSWKNIAKSFPDRTEVQCLHRWQ 121
>At2g20450.1 68415.m02387 60S ribosomal protein L14 (RPL14A)
Length = 134
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/64 (21%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = +3
Query: 324 LAVCDVGNNEFAFL-RPQKRSLGRSLKRLGSNDVIFSSDYVPNSMNVLNKVKEAIPRNKF 500
+ + DV + A + P + +LKRL D++ + VP ++ +++A +NK+
Sbjct: 25 VVIVDVVDQNRALVDAPDMERIQMNLKRLSLTDIVIDINRVPKKKVLIEAMEKADVKNKW 84
Query: 501 KANT 512
+ ++
Sbjct: 85 EKSS 88
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 27.5 bits (58), Expect = 9.4
Identities = 20/74 (27%), Positives = 36/74 (48%), Gaps = 2/74 (2%)
Frame = +3
Query: 306 PQLCHSLAVCDVGNNEFAFLRPQKRS-LGRSLKRLGSNDVIFSSDYVPNSMNVLNKVKEA 482
P+ H+L DV N+F L P+ + L+ L ++ F + +P+S+ +N ++
Sbjct: 409 PKSAHNLLFLDVSANDFNHLFPENIGWIFPHLRYLNTSKNNFQEN-LPSSLGNMNGIQYM 467
Query: 483 -IPRNKFKANTTAS 521
+ RN F N S
Sbjct: 468 DLSRNSFHGNLPRS 481
>At1g17230.1 68414.m02099 leucine-rich repeat family protein /
protein kinase family protein contains protein kinase
domain, Pfam:PF00069; contains leucine-rich repeats,
Pfam:PF00560
Length = 1133
Score = 27.5 bits (58), Expect = 9.4
Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +3
Query: 297 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGRSLKRLG-SNDVIFSSDYVPNSMNVLNKV 473
P + LC SL V D+ N F + P + ++ +LK+L + +F S +P + L+ +
Sbjct: 108 PQDLSLCRSLEVLDLCTNRFHGVIPIQLTMIITLKKLYLCENYLFGS--IPRQIGNLSSL 165
Query: 474 KEAI 485
+E +
Sbjct: 166 QELV 169
>At1g05460.1 68414.m00555 RNA helicase SDE3 (SDE3) identical to RNA
helicase SDE3 [Arabidopsis thaliana] GI:13811296
Length = 1002
Score = 27.5 bits (58), Expect = 9.4
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 5/67 (7%)
Frame = +3
Query: 306 PQLCHSLAVCDV--GNNEFAFLRPQKRSLGRSLKRLGSNDVIFSSD---YVPNSMNVLNK 470
P + + + CD GNN F R + + ++KRL +ND + D P V+ K
Sbjct: 663 PMVFYGIQGCDEREGNNPSWFNRIEISKVIETIKRLTANDCVQEEDIGVITPYRQQVM-K 721
Query: 471 VKEAIPR 491
+KE + R
Sbjct: 722 IKEVLDR 728
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,141,343
Number of Sequences: 28952
Number of extensions: 296548
Number of successful extensions: 864
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 864
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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