Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120294.Seq
(885 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35910.1 68418.m04312 3'-5' exonuclease domain-containing pro... 96 2e-20
At1g54440.1 68414.m06210 3'-5' exonuclease domain-containing pro... 93 2e-19
At2g32415.1 68415.m03961 3'-5' exonuclease domain-containing pro... 91 7e-19
At1g56310.1 68414.m06473 3'-5' exonuclease domain-containing pro... 39 0.005
At5g24340.1 68418.m02868 3'-5' exonuclease domain-containing pro... 33 0.25
At2g25910.1 68415.m03109 3'-5' exonuclease domain-containing pro... 30 1.8
At1g17870.1 68414.m02211 expressed protein contains 6 transmembr... 30 1.8
At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2) sim... 29 5.4
At4g11720.1 68417.m01870 hypothetical protein 29 5.4
At5g25280.2 68418.m02999 serine-rich protein-related contains so... 28 7.2
At5g25280.1 68418.m02998 serine-rich protein-related contains so... 28 7.2
At3g24240.1 68416.m03042 leucine-rich repeat transmembrane prote... 28 7.2
At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin... 28 7.2
At1g58230.1 68414.m06618 WD-40 repeat family protein / beige-rel... 28 9.5
>At5g35910.1 68418.m04312 3'-5' exonuclease domain-containing
protein / helicase and RNase D C-terminal
domain-containing protein / HRDC domain-containing
protein low similarity to SP|Q01780
Polymyositis/scleroderma autoantigen 2 {Homo sapiens};
contains Pfam profiles PF00570: HRDC domain, PF01612:
3'-5' exonuclease
Length = 838
Score = 96.3 bits (229), Expect = 2e-20
Identities = 47/86 (54%), Positives = 60/86 (69%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 QGITCLIQISTDEGGDFIIDALAVREHIHK-LNVVFTDPKKLKVFHGADSDVLWLQRDFG 178
QG+TCL+QIST D+I+D +R HI L +F DPKK KV HGAD D++WLQRDFG
Sbjct: 263 QGLTCLMQIST-RTEDYIVDTFKLRIHIGPYLREIFKDPKKKKVMHGADRDIIWLQRDFG 321
Query: 179 VYLVGLFDTYHAAKSLGLPALSLKFL 256
+Y+ LFDT A++ L L SL+FL
Sbjct: 322 IYVCNLFDTGQASRVLNLERNSLEFL 347
Score = 83.8 bits (198), Expect = 1e-16
Identities = 40/104 (38%), Positives = 62/104 (59%), Gaps = 3/104 (2%)
Frame = +1
Query: 214 SQVTRSARAFAQISLMKYCGVDTDKTYRLADWRIRPLPDVLIKYARMDTHYLLYVWRVMK 393
S+V R + L +CGV +K Y+ ADWRIRPLP+ + +YAR DTHYLLY++ ++K
Sbjct: 334 SRVLNLERNSLEFLLQHFCGVTANKEYQNADWRIRPLPEEMTRYAREDTHYLLYIYDLIK 393
Query: 394 ---NQIIEKNAGQTNMLLSVFEDSRQTCASTYNKEVIHDESPYH 516
++ + +A + LL V++ S C Y KE++ + S H
Sbjct: 394 LELQRMAKDDAHTDSPLLEVYKRSYDVCTQLYEKELLTENSYLH 437
Score = 50.4 bits (115), Expect = 2e-06
Identities = 24/72 (33%), Positives = 43/72 (59%)
Frame = +3
Query: 540 FDNQQMAALKMLYKWRDSQARQLDESTTYLLPNHMLLSLSENLPRELQGVNACCDPMPPF 719
F+ Q+A + L +WRD AR DEST Y+LPN +LL +++ +P + + P+
Sbjct: 446 FNAAQLAIVAGLCEWRDFIARAEDESTGYVLPNKVLLEIAKEMPDSVGKLRRMLKSKHPY 505
Query: 720 VKQNVITIHRMI 755
+++NV ++ +I
Sbjct: 506 IERNVDSVVSVI 517
>At1g54440.1 68414.m06210 3'-5' exonuclease domain-containing
protein / helicase and RNase D C-terminal
domain-containing protein / HRDC domain-containing
protein similar to SP|Q01780 Polymyositis/scleroderma
autoantigen 2 {Homo sapiens}; contains Pfam profiles
PF00570: HRDC domain, PF01612: 3'-5' exonuclease
Length = 738
Score = 93.1 bits (221), Expect = 2e-19
Identities = 46/86 (53%), Positives = 60/86 (69%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 QGITCLIQISTDEGGDFIIDALAVREHIHK-LNVVFTDPKKLKVFHGADSDVLWLQRDFG 178
QG+TCL+QIST D+I+D + +HI L +F DPKK KV HGAD D++WLQRDFG
Sbjct: 263 QGLTCLMQIST-RTEDYIVDIFKLWDHIGPYLRELFKDPKKKKVIHGADRDIIWLQRDFG 321
Query: 179 VYLVGLFDTYHAAKSLGLPALSLKFL 256
+Y+ LFDT A++ L L SL+FL
Sbjct: 322 IYVCNLFDTGQASRVLKLERNSLEFL 347
Score = 76.2 bits (179), Expect = 3e-14
Identities = 33/63 (52%), Positives = 45/63 (71%)
Frame = +1
Query: 214 SQVTRSARAFAQISLMKYCGVDTDKTYRLADWRIRPLPDVLIKYARMDTHYLLYVWRVMK 393
S+V + R + L YCGV +K Y+ ADWRIRPLPDV+ +YAR DTHYLLY++ VM+
Sbjct: 334 SRVLKLERNSLEFLLKHYCGVAANKEYQKADWRIRPLPDVMKRYAREDTHYLLYIYDVMR 393
Query: 394 NQI 402
++
Sbjct: 394 MEL 396
Score = 44.0 bits (99), Expect = 1e-04
Identities = 21/65 (32%), Positives = 37/65 (56%)
Frame = +3
Query: 537 NFDNQQMAALKMLYKWRDSQARQLDESTTYLLPNHMLLSLSENLPRELQGVNACCDPMPP 716
N + Q++ L+ L +WRD AR DEST Y+LPN L +++ +P + + P
Sbjct: 433 NLNAVQLSILQGLCEWRDRIARADDESTGYVLPNKTLFDIAKEMPIVVAQLRRLLKSKLP 492
Query: 717 FVKQN 731
++++N
Sbjct: 493 YLERN 497
>At2g32415.1 68415.m03961 3'-5' exonuclease domain-containing
protein similar to SP|Q12149 Exosome complex exonuclease
RRP6 (EC 3.1.13.-) (Ribosomal RNA processing protein 6)
{Saccharomyces cerevisiae}
Length = 880
Score = 91.5 bits (217), Expect = 7e-19
Identities = 44/84 (52%), Positives = 58/84 (69%)
Frame = +2
Query: 5 GITCLIQISTDEGGDFIIDALAVREHIHKLNVVFTDPKKLKVFHGADSDVLWLQRDFGVY 184
G T LIQIST E DF++D +A+ + + L VF+DP KVFHGAD+DV+WLQRDF +Y
Sbjct: 155 GFTALIQISTHEE-DFLVDTIALHDVMSILRPVFSDPNICKVFHGADNDVIWLQRDFHIY 213
Query: 185 LVGLFDTYHAAKSLGLPALSLKFL 256
+V +FDT A + L P SL +L
Sbjct: 214 VVNMFDTAKACEVLSKPQRSLAYL 237
Score = 56.4 bits (130), Expect = 2e-08
Identities = 33/97 (34%), Positives = 50/97 (51%), Gaps = 7/97 (7%)
Frame = +1
Query: 220 VTRSARAFAQISLMKYCGVDTDKTYRLADWRIRPLPDVLIKYARMDTHYLLYV----WRV 387
+++ R+ A + L CGV T+K + DWR RPL + +++YAR D HYLLY+
Sbjct: 227 LSKPQRSLAYL-LETVCGVATNKLLQREDWRQRPLSEEMVRYARTDAHYLLYIADSLTTE 285
Query: 388 MKNQIIEKNAG---QTNMLLSVFEDSRQTCASTYNKE 489
+K E ++ + + LL S TC Y KE
Sbjct: 286 LKQLATEDSSSPDDRFHFLLEASRRSNMTCLQLYTKE 322
Score = 31.1 bits (67), Expect = 1.0
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = +3
Query: 531 KKNFDNQQMAALKMLYKWRDSQARQLDESTTYLLPNHMLLSLSENLP 671
K N ++ L WRD R DEST Y+L + ++ LS P
Sbjct: 346 KSNISLNAEELVRKLCAWRDLMGRIHDESTRYVLSDQAIVGLSCKQP 392
>At1g56310.1 68414.m06473 3'-5' exonuclease domain-containing
protein contains Pfam profile PF01612: 3'-5' exonuclease
Length = 582
Score = 38.7 bits (86), Expect = 0.005
Identities = 17/55 (30%), Positives = 32/55 (58%)
Frame = +1
Query: 262 KYCGVDTDKTYRLADWRIRPLPDVLIKYARMDTHYLLYVWRVMKNQIIEKNAGQT 426
K GV +KT R +DW RPL ++YA +D L++++R +++ ++ +T
Sbjct: 504 KILGVSLNKTRRNSDWEQRPLSQNQLEYAALDAAVLIHIFRHVRDHPPHDSSSET 558
>At5g24340.1 68418.m02868 3'-5' exonuclease domain-containing
protein contains Pfam profile PF01612: 3'-5' exonuclease
Length = 505
Score = 33.1 bits (72), Expect = 0.25
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 274 VDTDKTYRLADWRIRPLPDVLIKYARMDTHYLLYVWRVMKNQIIE 408
+ K + +DW RPL + YA D H LL ++ V + ++E
Sbjct: 163 ISLSKELQCSDWSYRPLTEEQKLYAATDAHCLLQIFDVFEAHLVE 207
>At2g25910.1 68415.m03109 3'-5' exonuclease domain-containing
protein / K homology domain-containing protein / KH
domain-containing protein contains Pfam profiles
PF01612: 3'-5' exonuclease, PF00013: KH domain
Length = 341
Score = 30.3 bits (65), Expect = 1.8
Identities = 10/36 (27%), Positives = 23/36 (63%)
Frame = +1
Query: 307 WRIRPLPDVLIKYARMDTHYLLYVWRVMKNQIIEKN 414
W RP+ +++I+ A D +LLY++ M ++ +++
Sbjct: 191 WTYRPMTELMIRAAADDVRFLLYLYHKMMGKLNQRS 226
>At1g17870.1 68414.m02211 expressed protein contains 6 transmembrane
domains; similar to predicted metalloproteases
Length = 573
Score = 30.3 bits (65), Expect = 1.8
Identities = 25/95 (26%), Positives = 43/95 (45%), Gaps = 10/95 (10%)
Frame = -1
Query: 258 QRNLSESAGRPSDLAAWYVSKRPTKYT-------PKSR*S---QRTSLSAPWNTFSFLGS 109
+ LSE+AGR D+ W++ +R + T PK+ + T LS PW S +
Sbjct: 222 EAKLSEAAGR--DVVVWFMEERSNEITKQVCMVQPKAEIDLQFESTRLSTPWGYVSAIAL 279
Query: 108 VKTTFSLCMCSLTARASMIKSPPSSVDI*MRHVMP 4
TTF ++ + P ++ D + +V+P
Sbjct: 280 CVTTFG----TIALMSGFFLKPDATFDDYIANVVP 310
>At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2)
similar to importin alpha 2 [Capsicum annuum]
GI:13752562; contains Pfam profiles PF01749: Importin
beta binding domain, PF00514:
Armadillo/beta-catenin-like repeat; non-consensus GG
donor splice site at exon 1 and 6; CT acceptor splice
site at exon 2
Length = 444
Score = 28.7 bits (61), Expect = 5.4
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = +3
Query: 342 VRSHGYALSFVRLA--RYEESNNREECWTNEYATVGLRGQ 455
V SHG LS + L +++S +E CWT T G R Q
Sbjct: 327 VISHGALLSLLSLLTHNHKKSIKKEACWTISNITAGNRDQ 366
>At4g11720.1 68417.m01870 hypothetical protein
Length = 658
Score = 28.7 bits (61), Expect = 5.4
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +2
Query: 749 YDLI-VRELPKDAQIFHITVRHKEHDERRD 835
YDL +R++P Q +H+T R EHD D
Sbjct: 137 YDLTYIRDVPYKPQEYHVTTRKCEHDAGPD 166
>At5g25280.2 68418.m02999 serine-rich protein-related contains some
similarity to serine-rich proteins
Length = 220
Score = 28.3 bits (60), Expect = 7.2
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = -1
Query: 354 HASVLDQHVRKRTNAPVRQTIRFIGVNAAILHQRNLSESAGRPSD 220
H S V T+AP+ Q+ R+ N +I R+++ S+ +PS+
Sbjct: 71 HRSASPTRVNLFTSAPMTQSFRYSIDNRSISPNRSIAVSSNKPSN 115
>At5g25280.1 68418.m02998 serine-rich protein-related contains some
similarity to serine-rich proteins
Length = 220
Score = 28.3 bits (60), Expect = 7.2
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = -1
Query: 354 HASVLDQHVRKRTNAPVRQTIRFIGVNAAILHQRNLSESAGRPSD 220
H S V T+AP+ Q+ R+ N +I R+++ S+ +PS+
Sbjct: 71 HRSASPTRVNLFTSAPMTQSFRYSIDNRSISPNRSIAVSSNKPSN 115
>At3g24240.1 68416.m03042 leucine-rich repeat transmembrane protein
kinase, putative similar to CLV1 receptor kinase
GB:AAB58929 from [Arabidopsis thaliana]
Length = 1141
Score = 28.3 bits (60), Expect = 7.2
Identities = 11/37 (29%), Positives = 24/37 (64%)
Frame = +3
Query: 522 IRSKKNFDNQQMAALKMLYKWRDSQARQLDESTTYLL 632
IR+++N DN++ + L YKW+ + ++L+ S ++
Sbjct: 747 IRARRNIDNERDSELGETYKWQFTPFQKLNFSVDQII 783
>At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling
(PFI) almost identical to tubulin folding cofactor E
(Pfifferling; PFI) GI:20514267 from [Arabidopsis
thaliana]; identical to cDNA tubulin folding cofactor E,
GI:20514266
Length = 531
Score = 28.3 bits (60), Expect = 7.2
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 627 LLPNHMLLSLSENLPRELQGVNACCDPMPPFVKQNV 734
+LPN LL L+ NL + + + A C+ +P N+
Sbjct: 158 ILPNLKLLDLTGNLISDWEEIGALCEQLPALTTLNL 193
>At1g58230.1 68414.m06618 WD-40 repeat family protein /
beige-related contains Pfam PF00400: WD domain, G-beta
repeat; similar to Lipopolysaccharide-responsive and
beige-like anchor protein (CDC4-like protein)
(Beige-like protein) (SP:P50851) [Homo sapiens}
Length = 1280
Score = 27.9 bits (59), Expect = 9.5
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = +3
Query: 459 SDLRLYIQ*RGDSRREPIPLYIRSKKNFDNQQMAALKMLYKWRDSQ 596
SD +Q G+ E + + SKK FD + LK + W +++
Sbjct: 224 SDRTRQVQALGERNNETLSIECNSKKAFDGELQNVLKTVVTWDENR 269
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,282,961
Number of Sequences: 28952
Number of extensions: 407974
Number of successful extensions: 1095
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1055
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1092
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2071520424
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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