BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120289.Seq (752 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 40 4e-04 SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 32 0.10 SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 31 0.13 SPCC24B10.08c |||histone acetyltransferase complex subunit Ada2 ... 31 0.18 SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G family|Schizosaccha... 29 0.71 SPAC3H8.04 |||chromosome segregation protein|Schizosaccharomyces... 29 0.94 SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 29 0.94 SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 28 1.2 SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 28 1.2 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 27 2.9 SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyce... 27 3.8 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 27 3.8 SPBC21B10.08c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 26 5.0 SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 26 5.0 SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 26 6.6 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 6.6 SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 26 6.6 SPAC3C7.02c |||protein kinase inhibitor |Schizosaccharomyces pom... 26 6.6 SPBC428.17c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.8 SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizo... 25 8.8 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 25 8.8 SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|c... 25 8.8 SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||... 25 8.8 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 39.9 bits (89), Expect = 4e-04 Identities = 17/52 (32%), Positives = 29/52 (55%), Gaps = 2/52 (3%) Frame = +2 Query: 344 ECNVCKEISTDERFLKPKEC--CEYAICNACCVTMWKTATTHAKCPACRTSY 493 EC +C + + ER L K C C + +A C+ W ++ ++CP CR+S+ Sbjct: 1557 ECAICYSVLSVERTLPNKRCGTCRHKF-HASCLYKWFKSSNSSRCPLCRSSF 1607 >SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 269 Score = 31.9 bits (69), Expect = 0.10 Identities = 15/48 (31%), Positives = 22/48 (45%) Frame = +2 Query: 341 YECNVCKEISTDERFLKPKECCEYAICNACCVTMWKTATTHAKCPACR 484 Y+C +C + S + P C + CN C ++ T KCP CR Sbjct: 204 YKCVICLD-SPENLSCTP---CGHIFCNFCILSALGTTAATQKCPVCR 247 >SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 489 Score = 31.5 bits (68), Expect = 0.13 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = +2 Query: 347 CNVC-KEISTDERFLKPKECCEYAICNACCVTMWKTATTHAKCPACRTSYK*ARNQNEWR 523 C +C +EI ++ KP +C Y +C C + + + +CPACR Y +WR Sbjct: 18 CPLCMEEIDISDKNFKPCQC-GYRVCRFCWHHIKEDL--NGRCPACRRLY--TEENVQWR 72 >SPCC24B10.08c |||histone acetyltransferase complex subunit Ada2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 437 Score = 31.1 bits (67), Expect = 0.18 Identities = 13/45 (28%), Positives = 20/45 (44%) Frame = +2 Query: 341 YECNVCKEISTDERFLKPKECCEYAICNACCVTMWKTATTHAKCP 475 Y CNVC + T ++ EC ++ +C C + T H P Sbjct: 6 YHCNVCAQDITRSIHIRCVECVDFDLCIPCFTSGASLGTHHPSHP 50 >SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G family|Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 29.1 bits (62), Expect = 0.71 Identities = 16/40 (40%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Frame = +3 Query: 570 LTSYFDLKDNEHVPSEPM--AFIRNYLNCTFDLLDDAVLM 683 ++++ DL DN H+P + M I LN TFD D LM Sbjct: 332 ISNFLDLNDNFHLPLQIMDPRKIAQELNLTFDGFQDYCLM 371 >SPAC3H8.04 |||chromosome segregation protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 338 Score = 28.7 bits (61), Expect = 0.94 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +3 Query: 51 HHFIFDAQRIIQCIKEIEGAYG 116 HH+++D+QRII + + G YG Sbjct: 302 HHYLYDSQRIIFVQQSLGGLYG 323 >SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase Alg10|Schizosaccharomyces pombe|chr 1|||Manual Length = 445 Score = 28.7 bits (61), Expect = 0.94 Identities = 20/76 (26%), Positives = 35/76 (46%), Gaps = 5/76 (6%) Frame = -3 Query: 444 HMVTQHALHIAYSQHSFGFKNLSSVDISLQTLHSYKTEL-----STNKFITLTVLLIVSS 280 H + H I Y F F + S I HS +++L S F+ ++VLL+++ Sbjct: 250 HQASIHLSQINYFLWFFFFFSFPSYIIKYLMSHSRRSKLLSAVFSKKSFLIVSVLLLIAH 309 Query: 279 FSINNRQFVLTRSRTF 232 F+ F+L +R + Sbjct: 310 FNTIFHPFILADNRHY 325 >SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 232 Score = 28.3 bits (60), Expect = 1.2 Identities = 20/64 (31%), Positives = 25/64 (39%), Gaps = 1/64 (1%) Frame = +2 Query: 332 SVLYECNVCKEISTDERFLKPKEC-CEYAICNACCVTMWKTATTHAKCPACRTSYK*ARN 508 S LYECN C+EI + C C Y + CC + H C C T + A Sbjct: 179 SNLYECNACREI-----VIAGYVCDCGYCLHVYCCKHL-----AHVNCINCNTPWANATV 228 Query: 509 QNEW 520 W Sbjct: 229 IGRW 232 >SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 28.3 bits (60), Expect = 1.2 Identities = 16/59 (27%), Positives = 29/59 (49%) Frame = +2 Query: 341 YECNVCKEISTDERFLKPKECCEYAICNACCVTMWKTATTHAKCPACRTSYK*ARNQNE 517 Y+ +C +S D + +P C I + C+ + T + +CP C T+Y+ A+ E Sbjct: 21 YDKKLCS-LSNDS-WRRPCRCS--LIAHESCLISYITRSGSTRCPQCLTAYRIAKPPKE 75 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 27.1 bits (57), Expect = 2.9 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -1 Query: 545 FQSQQVAAATHFGCVLIYTMSC 480 FQ +A+A H GCV I+ +C Sbjct: 295 FQKSIIASAAHDGCVSIWNTAC 316 >SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 26.6 bits (56), Expect = 3.8 Identities = 8/28 (28%), Positives = 18/28 (64%) Frame = +3 Query: 393 QKNVANTLYATRAALPCGKRPPRTQSVQ 476 QKN+ + L++ + LPC + R ++++ Sbjct: 338 QKNIVDDLFSVKKPLPCSPKSKRVKTLE 365 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/29 (44%), Positives = 20/29 (68%) Frame = -3 Query: 387 KNLSSVDISLQTLHSYKTELSTNKFITLT 301 KNLS++ L+T +S+ ELS + ITL+ Sbjct: 303 KNLSTIQSPLKTSNSFFKELSPHSQITLS 331 >SPBC21B10.08c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 198 Score = 26.2 bits (55), Expect = 5.0 Identities = 20/73 (27%), Positives = 30/73 (41%), Gaps = 6/73 (8%) Frame = +3 Query: 489 RINKHATKMSGGGNLLTLERD--HFKYLFLTSYFDLKD---NEHVPSEPMAFIRNYLNCT 653 ++N+H K RD + Y+F +D K+ N H S P NYL T Sbjct: 30 KVNEHVLKSEPFTKTYYFARDAKNPDYIFGLELYDSKEVLENSHKTSNPFLAFANYLTST 89 Query: 654 F-DLLDDAVLMNY 689 L + L+N+ Sbjct: 90 HAPLAEPLQLLNF 102 >SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 860 Score = 26.2 bits (55), Expect = 5.0 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -2 Query: 508 VACLFIRCPARWTLCVRGGRFPHGNAARVA 419 V C IR P+ + LC G+ H +A ++A Sbjct: 337 VPCQLIRLPSDFVLCKMKGKKEHKDAFKIA 366 >SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 707 Score = 25.8 bits (54), Expect = 6.6 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = +1 Query: 379 KIFKAKRMLRIRYMQRVLRYHV 444 K+F +L I+Y+ +VL YHV Sbjct: 227 KVFNDLLLLAIQYVPKVLNYHV 248 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/55 (21%), Positives = 26/55 (47%) Frame = +2 Query: 308 VMNLFVDNSVLYECNVCKEISTDERFLKPKECCEYAICNACCVTMWKTATTHAKC 472 ++ F +NS + N+C+ S +F+ E+ +C C ++ + A + C Sbjct: 3258 ILKPFFENSKFTKQNLCESASIIVQFITDLTVGEFQLCLKCLLSFSQHAASLRIC 3312 >SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 2/48 (4%) Frame = -2 Query: 292 NSFQLFNKQQ--AICINSFTYICIEFIFLKQHAKEFLISFNASRNCFK 155 NSF L +++ +C C+ I L QH + LI + ++N F+ Sbjct: 286 NSFTLTRRRRHCRLCGRVICRFCVLEISLPQHPQPLLICMSCNQNYFR 333 >SPAC3C7.02c |||protein kinase inhibitor |Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 6.6 Identities = 23/97 (23%), Positives = 44/97 (45%) Frame = +3 Query: 393 QKNVANTLYATRAALPCGKRPPRTQSVQRAGHRINKHATKMSGGGNLLTLERDHFKYLFL 572 ++ + N++Y R+ P P+ ++++ + + A + L + R++FK F Sbjct: 145 KEKLLNSIYDVRSRDP---ESPKLITMEQ--ELVREEAACLVAEAQLTNITRENFKRAFT 199 Query: 573 TSYFDLKDNEHVPSEPMAFIRNYLNCTFDLLDDAVLM 683 L EH SE +A + Y DLLDD ++ Sbjct: 200 LHIGTLL--EH--SEKVAILCGYAKKILDLLDDTPIV 232 >SPBC428.17c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 602 Score = 25.4 bits (53), Expect = 8.8 Identities = 21/66 (31%), Positives = 31/66 (46%) Frame = +3 Query: 57 FIFDAQRIIQCIKEIEGAYGVRDRGNVIVFYPYLKQLRDALKLIKNSFACCFKNINSMQM 236 F + QRI+ I+ + GV GN YL + A K+ NSF C K+I + + Sbjct: 170 FAGEQQRIVDEIEYLVD--GVTFSGNSSASR-YLSLIGIAEKMFDNSFRLCLKSIRDVFL 226 Query: 237 YVNELI 254 + E I Sbjct: 227 RIFEEI 232 >SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 25.4 bits (53), Expect = 8.8 Identities = 12/40 (30%), Positives = 18/40 (45%) Frame = -2 Query: 280 LFNKQQAICINSFTYICIEFIFLKQHAKEFLISFNASRNC 161 L + I + F IC+ IF +++ I AS NC Sbjct: 202 LHENSREIVLTDFGSICLVPIFASNNSEAIAIQDKASENC 241 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 25.4 bits (53), Expect = 8.8 Identities = 8/18 (44%), Positives = 14/18 (77%) Frame = +2 Query: 338 LYECNVCKEISTDERFLK 391 +++CNV E+ D+RFL+ Sbjct: 865 VHDCNVISEVIPDQRFLR 882 >SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 25.4 bits (53), Expect = 8.8 Identities = 14/46 (30%), Positives = 26/46 (56%) Frame = +3 Query: 612 SEPMAFIRNYLNCTFDLLDDAVLMNYFNYLQSMQLKHLVGSTSTNI 749 SEPMAF+ +N + LL ++++ N ++ L+ L+ T T + Sbjct: 127 SEPMAFMSENVNLQYALL-NSIIDKMNNNIKKPNLEFLMPCTITKL 171 >SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1101 Score = 25.4 bits (53), Expect = 8.8 Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 3/72 (4%) Frame = -2 Query: 403 TFFWL*KSFIRRYFFTNIAFVQNRIVYEQIHNFNSFINSFQ---LFNKQQAICINSFTYI 233 T F + K FF ++ R +E + + F SFQ NK Q C+ SF+ + Sbjct: 542 TIFLISKYASSPSFFNEYPILKFRCDFENVRTMDRFYQSFQKALSMNKSQPSCL-SFSKL 600 Query: 232 CIEFIFLKQHAK 197 +F +++ Sbjct: 601 NDFVVFFNNYSR 612 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,158,107 Number of Sequences: 5004 Number of extensions: 66893 Number of successful extensions: 218 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 207 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 217 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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