Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120283.Seq
(816 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 126 4e-30
SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr... 81 2e-16
SPAPB24D3.01 ||SPAPB2C8.02|transcription factor |Schizosaccharom... 30 0.34
SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 29 1.0
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 29 1.0
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 2.4
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 26 5.6
SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Sc... 26 5.6
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 26 7.4
SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual 26 7.4
SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc... 25 9.7
SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb... 25 9.7
>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 126 bits (304), Expect = 4e-30
Identities = 62/100 (62%), Positives = 74/100 (74%), Gaps = 2/100 (2%)
Frame = -1
Query: 801 EEKYLIATSEQPILLSTGMNGFQESS--LPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQF 628
EE+YLIATSEQPI F++ S LP+KYAG STC+R+E GSHGRD GIFRVH F
Sbjct: 231 EERYLIATSEQPISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSHGRDAWGIFRVHAF 290
Query: 627 EKVEQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYRV 508
EK+EQFVLT P SW+ EMIN+AE+F K+L LPYR+
Sbjct: 291 EKIEQFVLTDP--EKSWEAFTEMINHAEDFYKSLELPYRI 328
Score = 111 bits (268), Expect = 9e-26
Identities = 51/85 (60%), Positives = 64/85 (75%)
Frame = -3
Query: 508 VNIVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRYGQTKKMNAATE 329
V IVSGALN+AA+KK DLEAWFP G ++ELVSCSNC +YQ+R L +R G K + +
Sbjct: 329 VAIVSGALNNAAAKKYDLEAWFPFQGEYKELVSCSNCTDYQSRNLEIRCGVKKMGDREKK 388
Query: 328 YVHMLNATMCATTRVICAILEVHQT 254
YVH LN+T+CAT R +C ILE +QT
Sbjct: 389 YVHCLNSTLCATERALCCILENYQT 413
Score = 25.8 bits (54), Expect = 7.4
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = -2
Query: 251 DGIKVPEILKPWLPKQYQELIPFVKPAP 168
DG+ VP++L+P++ + +PF K P
Sbjct: 415 DGVNVPKVLQPYMGG--KTFLPFTKELP 440
>SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 454
Score = 81.0 bits (191), Expect = 2e-16
Identities = 41/84 (48%), Positives = 53/84 (63%)
Frame = -3
Query: 508 VNIVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRYGQTKKMNAATE 329
+N+ + L +AS+K D+EAW P ++ E+ S SNCLEYQARRLL RY K +
Sbjct: 350 LNMPTAELGSSASQKYDIEAWMPARQSYGEITSASNCLEYQARRLLTRYRNDKD----SG 405
Query: 328 YVHMLNATMCATTRVICAILEVHQ 257
+VH LN T A R+I AILE HQ
Sbjct: 406 FVHTLNGTAAAIPRLIIAILENHQ 429
Score = 62.9 bits (146), Expect = 5e-11
Identities = 27/69 (39%), Positives = 46/69 (66%)
Frame = -1
Query: 714 KYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKVEQFVLTSPHDNASWQMMDEMINNAEEFC 535
K G+S +R+E G+ G++ RG++R+H+F KVE F T H + S +M +E++N +EF
Sbjct: 283 KVVGVSRAYRREAGARGKENRGLYRLHEFTKVELFAWT--HPSRSSEMFNEIVNFQKEFV 340
Query: 534 KALGLPYRV 508
+ L +P R+
Sbjct: 341 ETLKIPARI 349
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/19 (57%), Positives = 15/19 (78%), Gaps = 1/19 (5%)
Frame = -2
Query: 254 EDG-IKVPEILKPWLPKQY 201
EDG +KVPE L P++ K+Y
Sbjct: 431 EDGTVKVPETLVPYIHKEY 449
>SPAPB24D3.01 ||SPAPB2C8.02|transcription factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 594
Score = 30.3 bits (65), Expect = 0.34
Identities = 16/33 (48%), Positives = 19/33 (57%), Gaps = 5/33 (15%)
Frame = -3
Query: 424 RELVSCSNCLEYQ-----ARRLLVRYGQTKKMN 341
REL SC NC+ YQ ++RL Y TKK N
Sbjct: 23 RELPSCQNCIVYQEECHYSKRLKRSYSATKKKN 55
>SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 372
Score = 28.7 bits (61), Expect = 1.0
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 5/59 (8%)
Frame = +3
Query: 441 GNQASKSSFLEAAWFRAPDTM--FTHGTVVLVLCRT---LQHCLSFRPSFAKMRYHEEM 602
GN + ++ W+RAP+ M F + + L T L LS RP F YH ++
Sbjct: 193 GNPGFMTEYVATRWYRAPEIMLSFREYSKAIDLWSTGCILAEMLSARPLFPGKDYHSQI 251
>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1473
Score = 28.7 bits (61), Expect = 1.0
Identities = 8/20 (40%), Positives = 15/20 (75%)
Frame = -2
Query: 245 IKVPEILKPWLPKQYQELIP 186
+ + +L+PWLP + QE++P
Sbjct: 289 VAIVRVLRPWLPSKLQEVLP 308
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 27.5 bits (58), Expect = 2.4
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -3
Query: 472 SKKLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRY 362
SKKL+ E W P +F E++ ++ LE +++ L V +
Sbjct: 1394 SKKLEEEYWLPFLLSFNEIICEASDLEVRSKALKVLF 1430
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 26.2 bits (55), Expect = 5.6
Identities = 16/57 (28%), Positives = 26/57 (45%)
Frame = -1
Query: 666 GRDTRGIFRVHQFEKVEQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYRV*TSC 496
G DT I + EK+ + + W+ +D+ N ++ K + PYR TSC
Sbjct: 454 GSDTTWIINFFKSEKLTEIKI------GKWEKIDDKWNRKVQYIKPVAPPYRQ-TSC 503
>SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 776
Score = 26.2 bits (55), Expect = 5.6
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 317 AERDHVRHHQGHLRHTRSSPDEDGIKVPEILK 222
+ER H+ LRH +S ED +KV ++ K
Sbjct: 382 SERTHLLRELEELRHLATSAKEDFVKVEKLNK 413
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 25.8 bits (54), Expect = 7.4
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = -3
Query: 478 AASKKLDLEAWFPGSGAFRELV 413
+A+KK ++ W PGSG ++V
Sbjct: 156 SAAKKYGIQFWGPGSGIIHQIV 177
>SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 566
Score = 25.8 bits (54), Expect = 7.4
Identities = 10/33 (30%), Positives = 21/33 (63%)
Frame = -2
Query: 731 NHLFPSNMRDSPHASDKRLDLMDVTRVVSFEYI 633
N L N+ +SP S+KR+ ++D + ++ E++
Sbjct: 30 NELKNQNLFESPAESEKRVKVLDELQQITTEFV 62
>SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 745
Score = 25.4 bits (53), Expect = 9.7
Identities = 20/83 (24%), Positives = 35/83 (42%), Gaps = 2/83 (2%)
Frame = -1
Query: 807 VVEEKYLIATSEQPILLSTGMNGFQESSLPIKYAGLSTCFRQEVGSHGRDTRGIF--RVH 634
++ E LI P +L + F + +L +KY L R V SH + + R
Sbjct: 463 LISEHILIIPRLVPDVLRIAVKTFADETLEVKYQILELSVRLYVLSHSEEKQNDLESRDD 522
Query: 633 QFEKVEQFVLTSPHDNASWQMMD 565
+ +VL+ H + S+ + D
Sbjct: 523 VVSLLFNYVLSLIHFDMSYDLRD 545
>SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 510
Score = 25.4 bits (53), Expect = 9.7
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +3
Query: 570 SFAKMRYHEEMLEQIVPPFQIDVLERYHACHVHEIQPL 683
+ A R HE+ L + P +D R HA H+++ L
Sbjct: 19 TLADSRIHEQYLVKAFPNEPVDYEGRMHAGHLNQTDQL 56
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,447,221
Number of Sequences: 5004
Number of extensions: 72516
Number of successful extensions: 204
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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