SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120283.Seq
         (816 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g27470.1 68418.m03281 seryl-tRNA synthetase / serine--tRNA li...   134   5e-32
At1g11870.2 68414.m01369 seryl-tRNA synthetase, putative / serin...    75   6e-14
At1g11870.1 68414.m01368 seryl-tRNA synthetase, putative / serin...    75   6e-14
At1g11870.3 68414.m01367 seryl-tRNA synthetase, putative / serin...    73   2e-13
At2g47800.1 68415.m05966 glutathione-conjugate transporter (MRP4...    29   4.9  
At3g10980.2 68416.m01324 expressed protein                             28   8.5  
At3g10980.1 68416.m01325 expressed protein                             28   8.5  

>At5g27470.1 68418.m03281 seryl-tRNA synthetase / serine--tRNA
           ligase identical to SP|Q39230 Seryl-tRNA synthetase (EC
           6.1.1.11) (Serine--tRNA ligase) (SerRS) {Arabidopsis
           thaliana}
          Length = 451

 Score =  134 bits (325), Expect = 5e-32
 Identities = 57/98 (58%), Positives = 78/98 (79%)
 Frame = -1

Query: 801 EEKYLIATSEQPILLSTGMNGFQESSLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEK 622
           ++KYLIAT+EQP+           + LP++YAG S+CFR+E GSHGRDT GIFRVHQFEK
Sbjct: 231 DDKYLIATAEQPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK 290

Query: 621 VEQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYRV 508
           +EQF +T P++NASW+M+DEM+ N+E+F +AL LPY++
Sbjct: 291 IEQFCITGPNENASWEMLDEMMKNSEDFYQALKLPYQI 328



 Score =  123 bits (297), Expect = 1e-28
 Identities = 58/93 (62%), Positives = 68/93 (73%)
 Frame = -3

Query: 535 QSTRTTVPCVNIVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRYGQ 356
           Q+ +     V+IVSGALN AA+KK DLEAWFP S  FRELVSCSNC +YQARRL +RYGQ
Sbjct: 320 QALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELVSCSNCTDYQARRLEIRYGQ 379

Query: 355 TKKMNAATEYVHMLNATMCATTRVICAILEVHQ 257
            K      +YVHMLN+T+ AT R IC ILE +Q
Sbjct: 380 KKSNEQTKQYVHMLNSTLTATERTICCILENYQ 412


>At1g11870.2 68414.m01369 seryl-tRNA synthetase, putative /
           serine--tRNA ligase, putative similar to PIR|T03949
           serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
           contains Pfam profiles PF00587: tRNA synthetase class II
           core domain (G, H, P, S and T), PF02403: Seryl-tRNA
           synthetase N-terminal domain
          Length = 514

 Score = 74.9 bits (176), Expect = 6e-14
 Identities = 43/99 (43%), Positives = 59/99 (59%), Gaps = 10/99 (10%)
 Frame = -3

Query: 508 VNIVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRY-----GQT--K 350
           +++ +  L   A +K D+EAW PG G F E+ S SNC +YQ+RRL +RY      QT  K
Sbjct: 394 LDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPK 453

Query: 349 KMNA---ATEYVHMLNATMCATTRVICAILEVHQTRTAS 242
           K  A   AT++VH LNAT CA  R++  +LE +Q    S
Sbjct: 454 KGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGS 492



 Score = 73.7 bits (173), Expect = 1e-13
 Identities = 38/96 (39%), Positives = 57/96 (59%)
 Frame = -1

Query: 798 EKYLIATSEQPILLSTGMNGFQESSLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKV 619
           ++ LI T+E P+      +   ES+LP+KY   S CFR E G+ G  T+G++RVHQF K 
Sbjct: 299 DQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKA 358

Query: 618 EQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYR 511
           E FV+  P D+ S+    E+I   E+   +LGL ++
Sbjct: 359 EMFVICQPEDSESFH--QELIQIEEDLFTSLGLHFK 392


>At1g11870.1 68414.m01368 seryl-tRNA synthetase, putative /
           serine--tRNA ligase, putative similar to PIR|T03949
           serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
           contains Pfam profiles PF00587: tRNA synthetase class II
           core domain (G, H, P, S and T), PF02403: Seryl-tRNA
           synthetase N-terminal domain
          Length = 512

 Score = 74.9 bits (176), Expect = 6e-14
 Identities = 43/99 (43%), Positives = 59/99 (59%), Gaps = 10/99 (10%)
 Frame = -3

Query: 508 VNIVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRY-----GQT--K 350
           +++ +  L   A +K D+EAW PG G F E+ S SNC +YQ+RRL +RY      QT  K
Sbjct: 392 LDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPK 451

Query: 349 KMNA---ATEYVHMLNATMCATTRVICAILEVHQTRTAS 242
           K  A   AT++VH LNAT CA  R++  +LE +Q    S
Sbjct: 452 KGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGS 490



 Score = 73.7 bits (173), Expect = 1e-13
 Identities = 38/96 (39%), Positives = 57/96 (59%)
 Frame = -1

Query: 798 EKYLIATSEQPILLSTGMNGFQESSLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKV 619
           ++ LI T+E P+      +   ES+LP+KY   S CFR E G+ G  T+G++RVHQF K 
Sbjct: 297 DQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKA 356

Query: 618 EQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYR 511
           E FV+  P D+ S+    E+I   E+   +LGL ++
Sbjct: 357 EMFVICQPEDSESFH--QELIQIEEDLFTSLGLHFK 390


>At1g11870.3 68414.m01367 seryl-tRNA synthetase, putative /
           serine--tRNA ligase, putative similar to PIR|T03949
           serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
           contains Pfam profiles PF00587: tRNA synthetase class II
           core domain (G, H, P, S and T), PF02403: Seryl-tRNA
           synthetase N-terminal domain
          Length = 402

 Score = 72.9 bits (171), Expect = 2e-13
 Identities = 38/95 (40%), Positives = 56/95 (58%)
 Frame = -1

Query: 798 EKYLIATSEQPILLSTGMNGFQESSLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKV 619
           ++ LI T+E P+      +   ES+LP+KY   S CFR E G+ G  T+G++RVHQF K 
Sbjct: 299 DQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKA 358

Query: 618 EQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPY 514
           E FV+  P D+ S+    E+I   E+   +LGL +
Sbjct: 359 EMFVICQPEDSESFH--QELIQIEEDLFTSLGLHF 391


>At2g47800.1 68415.m05966 glutathione-conjugate transporter (MRP4)
            identical to AtMRP4 GI:2959767 from [Arabidopsis
            thaliana]
          Length = 1516

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 15/36 (41%), Positives = 22/36 (61%)
 Frame = +2

Query: 329  FSGSVHLFRLTVPYKQSSCLVLQTVTTRDKFPERTG 436
            F G+VHL  L V Y+ ++ LVL+ +T   K  E+ G
Sbjct: 1270 FHGNVHLEDLKVRYRPNTPLVLKGITLDIKGGEKVG 1305


>At3g10980.2 68416.m01324 expressed protein 
          Length = 286

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -3

Query: 502 IVSGALNHAASKKLDLEAWF 443
           +++G LNHA  KK   +AWF
Sbjct: 148 VMTGMLNHALPKKSQRDAWF 167


>At3g10980.1 68416.m01325 expressed protein 
          Length = 557

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -3

Query: 502 IVSGALNHAASKKLDLEAWF 443
           +++G LNHA  KK   +AWF
Sbjct: 148 VMTGMLNHALPKKSQRDAWF 167


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,346,782
Number of Sequences: 28952
Number of extensions: 400331
Number of successful extensions: 1128
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1070
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1124
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -