BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120281.Seq (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 42 7e-05 SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||M... 29 0.42 SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce... 26 3.9 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 25 5.2 SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyce... 25 5.2 SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 6.8 SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 25 6.8 SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.0 >SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 41.5 bits (93), Expect = 7e-05 Identities = 18/34 (52%), Positives = 22/34 (64%) Frame = +2 Query: 149 KFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKT 250 K+ ID T D I DV FEKYL + +KV+GKT Sbjct: 11 KYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKT 44 Score = 41.5 bits (93), Expect = 7e-05 Identities = 23/65 (35%), Positives = 33/65 (50%), Gaps = 1/65 (1%) Frame = +1 Query: 241 GQNKYLSNHVVVARD-KTKVAITADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDAY 417 G+ L + VVV+R+ +K+A+ A I FS DWLRVV++ Y Sbjct: 42 GKTGNLGSSVVVSREGSSKIAVIAHIDFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVY 101 Query: 418 ELRYF 432 ELRY+ Sbjct: 102 ELRYY 106 >SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 29.1 bits (62), Expect = 0.42 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = -2 Query: 133 LDAFAADFATLHSFFASLLVQLGFLSRRF 47 +D + DFAT H+ + + L +GFLS F Sbjct: 87 IDLYILDFATQHNLYVASLRNMGFLSLVF 115 >SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 25.8 bits (54), Expect = 3.9 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +3 Query: 234 KLRAKQIPKQSRCRRQG*DESRYHRRHSFFKEVPEVFNKTLPQEEQF 374 +LR + SR R + + ++ R SF +VPE + TLP+ F Sbjct: 125 QLRRDLRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDF 171 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 25.4 bits (53), Expect = 5.2 Identities = 12/44 (27%), Positives = 24/44 (54%), Gaps = 1/44 (2%) Frame = -3 Query: 216 YFSKLPTSKILSSAGCVQSIVNFKLIFR-LMPLPRILPPFTPFL 88 ++ + ++ G +++I ++F L L RI+ PFTPF+ Sbjct: 729 WYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPFI 772 >SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyces pombe|chr 3|||Manual Length = 554 Score = 25.4 bits (53), Expect = 5.2 Identities = 8/22 (36%), Positives = 16/22 (72%) Frame = -1 Query: 344 KYFRYLFEKGMSAVIATFVLSL 279 K+FRY+F ++ ++ FVL++ Sbjct: 198 KWFRYIFRVSVAVILLDFVLNM 219 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 25.0 bits (52), Expect = 6.8 Identities = 11/30 (36%), Positives = 20/30 (66%) Frame = +2 Query: 194 DVGNFEKYLKEHVKVEGKTNT*AITLSSPG 283 + GN +KY + ++KV GK ++ +T +PG Sbjct: 955 EAGNLKKYDQPNLKVSGKNDS-FVTHHTPG 983 >SPBC4B4.04 |||translation initiation factor eIF2A |Schizosaccharomyces pombe|chr 2|||Manual Length = 576 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = -3 Query: 330 PL*KRNVCGDSDFRLIPGDDNVIA*VFV 247 P+ K V G SDF L PG ++ +A VF+ Sbjct: 159 PIAKLRVEGISDFALSPGQNHAVA-VFI 185 >SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 350 Score = 24.6 bits (51), Expect = 9.0 Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 7/53 (13%) Frame = +2 Query: 191 LDVGNFEKYLKEHVKVEG------KTNT*AITLS-SPGIRRKSLSPQTFLFQR 328 LD +F K +H++V+ +++ I+ S P +RRKSL P+ +FQR Sbjct: 111 LDQRSFSKTPNKHIEVKNLKDLCSPSHSGRISKSLCPVLRRKSLLPKPKMFQR 163 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,792,323 Number of Sequences: 5004 Number of extensions: 31679 Number of successful extensions: 78 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 77 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 78 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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