BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120278.Seq (785 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein ... 30 2.0 At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 4.6 At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 4.6 At4g25320.1 68417.m03643 DNA-binding protein-related contains Pf... 29 4.6 At3g57300.1 68416.m06378 transcriptional activator, putative sim... 29 4.6 At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 29 4.6 At3g51280.1 68416.m05613 male sterility MS5, putative similar to... 28 6.1 At5g55390.1 68418.m06901 hydroxyproline-rich glycoprotein family... 28 8.1 At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 28 8.1 >At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein contains Pfam PF00096: Zinc finger, C2H2 type Length = 387 Score = 29.9 bits (64), Expect = 2.0 Identities = 18/77 (23%), Positives = 35/77 (45%) Frame = +3 Query: 348 IMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDLTQKLTVPTQIWR 527 I+KSK P ++EW+ ++++ TG + T D +++ + ++ VP Sbjct: 17 ILKSKAPAVKWIKEWVPQDIV----ATGGKCHLHKWVTEDTFSRLKEKEKEPDVPEPEPE 72 Query: 528 KQPIAHFVCQRNDCGST 578 F+C + CG T Sbjct: 73 PTTEILFLCSYDGCGKT 89 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 28.7 bits (61), Expect = 4.6 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +1 Query: 61 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 201 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 822 Score = 28.7 bits (61), Expect = 4.6 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +1 Query: 61 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 201 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At4g25320.1 68417.m03643 DNA-binding protein-related contains Pfam domain PF03479: Domain of unknown function (DUF296), found in AT-hook motifs Pfam:PF02178 Length = 404 Score = 28.7 bits (61), Expect = 4.6 Identities = 13/34 (38%), Positives = 20/34 (58%) Frame = +2 Query: 206 TSTASTNRRLSMPTKSTPCSR*RGKAGRPAVFAP 307 T A+T L+MPT++T + + K GRP + P Sbjct: 64 TENAATPFSLTMPTENTSAEQLKKKRGRPRKYNP 97 >At3g57300.1 68416.m06378 transcriptional activator, putative similar to transcriptional activator SRCAP [Homo sapiens] GI:5106572; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain Length = 1507 Score = 28.7 bits (61), Expect = 4.6 Identities = 12/22 (54%), Positives = 17/22 (77%) Frame = -1 Query: 545 MSDRLLPPNLRWHRQFLRQIVN 480 +++RLL LRW RQFL ++VN Sbjct: 1012 VAERLLFSILRWERQFLDELVN 1033 >At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative similar to GTL1 [Arabidopsis thaliana] GI:2664198 Length = 669 Score = 28.7 bits (61), Expect = 4.6 Identities = 20/47 (42%), Positives = 24/47 (51%), Gaps = 2/47 (4%) Frame = -1 Query: 650 RHAVGHCAVSRRAFSQSCRAVSASRRATIISLANKMSDRL--LPPNL 516 R + A SQ RA SASR A IISL K++ LPP+L Sbjct: 308 RQEMARLAREHEVMSQE-RAASASRDAAIISLIQKITGHTIQLPPSL 353 >At3g51280.1 68416.m05613 male sterility MS5, putative similar to male sterility MS5 [Arabidopsis thaliana] GI:3859112; contains Pfam profile PF00515 TPR Domain Length = 430 Score = 28.3 bits (60), Expect = 6.1 Identities = 14/40 (35%), Positives = 19/40 (47%) Frame = +2 Query: 185 VNGQYVSTSTASTNRRLSMPTKSTPCSR*RGKAGRPAVFA 304 + G + + ST RRLSM K+T C K A+ A Sbjct: 363 IGGDHEGETNTSTRRRLSMEKKATECGLPDNKDFEDAIMA 402 >At5g55390.1 68418.m06901 hydroxyproline-rich glycoprotein family protein Length = 1332 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 15/28 (53%) Frame = -2 Query: 781 VSGCGPGSFLRRRNANSLLPTSHTARLW 698 V G G G +LR R+ N LLP + W Sbjct: 46 VDGSGLGFYLRGRSDNGLLPLHKLVKAW 73 >At4g37060.1 68417.m05248 patatin, putative similar to patatin-like latex allergen [Hevea brasiliensis][PMID:10589016]; contains patatin domain PF01734 Length = 414 Score = 27.9 bits (59), Expect = 8.1 Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 2/66 (3%) Frame = +3 Query: 276 AKQGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLLEEVIPQV-LCTGKYAPAV 449 A Q + YL L + + L KS L I+L E +L + Q+ + TG Y PA Sbjct: 313 ALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKMLTNRVMQMNIDTGTYEPAA 372 Query: 450 EMDTND 467 E ND Sbjct: 373 ENINND 378 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,915,583 Number of Sequences: 28952 Number of extensions: 392747 Number of successful extensions: 1071 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1046 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1071 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1765546400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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