BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120258.Seq (752 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 29 0.54 SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3... 29 0.71 SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 26 5.0 SPCC1450.07c |||D-amino acid oxidase |Schizosaccharomyces pombe|... 26 6.6 SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces p... 26 6.6 >SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 29.5 bits (63), Expect = 0.54 Identities = 14/43 (32%), Positives = 25/43 (58%) Frame = -2 Query: 742 GHSGVFSFKHSGGLKESRKFLSSLKVFTLAESLGGYESLAELP 614 G+ V SF GG +SRK +++LK+ + ++G ++L P Sbjct: 341 GYGAVLSFGAKGGPDQSRKVVNALKLASQLANVGDAKTLVIAP 383 >SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 449 Score = 29.1 bits (62), Expect = 0.71 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 202 SLINKQRHIFTVLTQTILMELMQEIICVAYKPKQNT 309 SL N Q+H++ VLTQ + + + C +K + T Sbjct: 217 SLSNYQQHLYQVLTQNVSLRFCSRLQCSFHKYDEKT 252 >SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 2344 Score = 26.2 bits (55), Expect = 5.0 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = -1 Query: 185 HYDKTIVVVFNCYFLWALKLSKAQPSLYLPLRTTE 81 H DK + FN Y+L A++ + SL L R +E Sbjct: 347 HQDKKDLETFNPYYLNAIESESSSQSLSLSQRASE 381 >SPCC1450.07c |||D-amino acid oxidase |Schizosaccharomyces pombe|chr 3|||Manual Length = 348 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%) Frame = -1 Query: 164 VVFNCYFLWALKLSKAQ-PSLY 102 VVFNC LWA KL + P +Y Sbjct: 183 VVFNCTGLWASKLGGVEDPDVY 204 >SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 25.8 bits (54), Expect = 6.6 Identities = 19/56 (33%), Positives = 28/56 (50%) Frame = -2 Query: 742 GHSGVFSFKHSGGLKESRKFLSSLKVFTLAESLGGYESLAELP*VQKLIHDSITPL 575 G V + H+ GL + + K TLAE+L G++ LAE QK+I P+ Sbjct: 360 GIPSVLKYLHAEGLIDGSNITVTGK--TLAENLRGFKDLAE---GQKIIRPLSNPI 410 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,873,937 Number of Sequences: 5004 Number of extensions: 54745 Number of successful extensions: 123 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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