SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120258.Seq
         (752 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloropla...    39   0.004
At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloropla...    38   0.005
At5g32405.1 68418.m03813 hypothetical protein                          28   5.8  
At3g60700.1 68416.m06791 expressed protein                             28   5.8  
At2g20780.1 68415.m02442 mannitol transporter, putative similar ...    28   7.7  

>At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloroplast
           / O-succinylhomoserine (Thiol)-lyase (CGS) identical to
           SP|P55217 Cystathionine gamma-synthase, chloroplast
           precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine
           (Thiol)-lyase) {Arabidopsis thaliana}
          Length = 563

 Score = 38.7 bits (86), Expect = 0.004
 Identities = 18/43 (41%), Positives = 27/43 (62%)
 Frame = -2

Query: 742 GHSGVFSFKHSGGLKESRKFLSSLKVFTLAESLGGYESLAELP 614
           G  GV SF+  G ++ + KF+ SLK+  +A S GG ES+ + P
Sbjct: 475 GFGGVVSFEIDGDIETTIKFVDSLKIPYIAPSFGGCESIVDQP 517


>At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloroplast,
           putative / O-succinylhomoserine (Thiol)-lyase, putative
           strong similarity to SP|P55217 Cystathionine
           gamma-synthase, chloroplast precursor (EC 4.2.99.9)
           (CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis
           thaliana}; contains Pfam profile PF01053: Cys/Met
           metabolism PLP-dependent enzyme
          Length = 412

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 19/41 (46%), Positives = 26/41 (63%)
 Frame = -2

Query: 742 GHSGVFSFKHSGGLKESRKFLSSLKVFTLAESLGGYESLAE 620
           G  G+ SF+  G LK + KF+ +LK+  LA S GG ESL +
Sbjct: 325 GIGGLISFEIDGDLKTTIKFIDALKIPYLAASFGGCESLVD 365


>At5g32405.1 68418.m03813 hypothetical protein
          Length = 256

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 10/32 (31%), Positives = 19/32 (59%)
 Frame = -1

Query: 347 NIKLIISLFCYLCVFCFGL*ATHIISCISSIK 252
           +I+L   +F Y C FC+    T ++SC+  ++
Sbjct: 219 SIQLWFLIFVYCCSFCYHTTVTLLLSCLQPVQ 250


>At3g60700.1 68416.m06791 expressed protein
          Length = 219

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 14/47 (29%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
 Frame = -1

Query: 287 ATHIISCISSIKIVWVS--TVKMCLCLFMSEHL*SRHYDKTIVVVFN 153
           AT I  C S + +VWV+   VK     F+S+ + + H   + +   N
Sbjct: 26  ATLIFCCFSQLLVVWVTLCAVKFSCSYFLSKSITTNHVPYSTIAFMN 72


>At2g20780.1 68415.m02442 mannitol transporter, putative similar to 
           mannitol transporter [Apium graveolens var. dulce]
           GI:12004316; contains Pfam profile PF00083: major
           facilitator superfamily protein
          Length = 526

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -1

Query: 299 FGL*ATHIISCISSIKIVWVSTVKMCLCLF 210
           F L AT +I  +    +++VST+ M LCLF
Sbjct: 352 FILFATFLIDSVGRKPLLYVSTIGMTLCLF 381


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,274,639
Number of Sequences: 28952
Number of extensions: 261465
Number of successful extensions: 463
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 459
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 463
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -