BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120258.Seq (752 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloropla... 39 0.004 At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloropla... 38 0.005 At5g32405.1 68418.m03813 hypothetical protein 28 5.8 At3g60700.1 68416.m06791 expressed protein 28 5.8 At2g20780.1 68415.m02442 mannitol transporter, putative similar ... 28 7.7 >At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloroplast / O-succinylhomoserine (Thiol)-lyase (CGS) identical to SP|P55217 Cystathionine gamma-synthase, chloroplast precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis thaliana} Length = 563 Score = 38.7 bits (86), Expect = 0.004 Identities = 18/43 (41%), Positives = 27/43 (62%) Frame = -2 Query: 742 GHSGVFSFKHSGGLKESRKFLSSLKVFTLAESLGGYESLAELP 614 G GV SF+ G ++ + KF+ SLK+ +A S GG ES+ + P Sbjct: 475 GFGGVVSFEIDGDIETTIKFVDSLKIPYIAPSFGGCESIVDQP 517 >At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloroplast, putative / O-succinylhomoserine (Thiol)-lyase, putative strong similarity to SP|P55217 Cystathionine gamma-synthase, chloroplast precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis thaliana}; contains Pfam profile PF01053: Cys/Met metabolism PLP-dependent enzyme Length = 412 Score = 38.3 bits (85), Expect = 0.005 Identities = 19/41 (46%), Positives = 26/41 (63%) Frame = -2 Query: 742 GHSGVFSFKHSGGLKESRKFLSSLKVFTLAESLGGYESLAE 620 G G+ SF+ G LK + KF+ +LK+ LA S GG ESL + Sbjct: 325 GIGGLISFEIDGDLKTTIKFIDALKIPYLAASFGGCESLVD 365 >At5g32405.1 68418.m03813 hypothetical protein Length = 256 Score = 28.3 bits (60), Expect = 5.8 Identities = 10/32 (31%), Positives = 19/32 (59%) Frame = -1 Query: 347 NIKLIISLFCYLCVFCFGL*ATHIISCISSIK 252 +I+L +F Y C FC+ T ++SC+ ++ Sbjct: 219 SIQLWFLIFVYCCSFCYHTTVTLLLSCLQPVQ 250 >At3g60700.1 68416.m06791 expressed protein Length = 219 Score = 28.3 bits (60), Expect = 5.8 Identities = 14/47 (29%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -1 Query: 287 ATHIISCISSIKIVWVS--TVKMCLCLFMSEHL*SRHYDKTIVVVFN 153 AT I C S + +VWV+ VK F+S+ + + H + + N Sbjct: 26 ATLIFCCFSQLLVVWVTLCAVKFSCSYFLSKSITTNHVPYSTIAFMN 72 >At2g20780.1 68415.m02442 mannitol transporter, putative similar to mannitol transporter [Apium graveolens var. dulce] GI:12004316; contains Pfam profile PF00083: major facilitator superfamily protein Length = 526 Score = 27.9 bits (59), Expect = 7.7 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = -1 Query: 299 FGL*ATHIISCISSIKIVWVSTVKMCLCLF 210 F L AT +I + +++VST+ M LCLF Sbjct: 352 FILFATFLIDSVGRKPLLYVSTIGMTLCLF 381 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,274,639 Number of Sequences: 28952 Number of extensions: 261465 Number of successful extensions: 463 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 459 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 463 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1672953192 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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