BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120254.Seq (606 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g37310.1 68418.m04481 endomembrane protein 70, putative multi... 31 0.45 At1g19930.1 68414.m02498 kelch repeat-containing F-box family pr... 30 1.4 At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 29 3.2 At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 29 3.2 At3g12500.1 68416.m01556 basic endochitinase identical to basic ... 28 4.2 At5g10520.1 68418.m01218 protein kinase family protein contains ... 28 5.5 At4g25710.1 68417.m03701 kelch repeat-containing F-box family pr... 27 9.6 At2g16390.1 68415.m01876 SNF2 domain-containing protein / helica... 27 9.6 At1g62600.1 68414.m07062 flavin-containing monooxygenase family ... 27 9.6 >At5g37310.1 68418.m04481 endomembrane protein 70, putative multispanning membrane protein, Homo sapiens, EMBL:HSU94831 Length = 564 Score = 31.5 bits (68), Expect = 0.45 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 3/56 (5%) Frame = +1 Query: 7 KTSPILYKYLWCNKMVFDVYYNGYYVEK---KFSKEFLIHIAPDLKNSVDWNGSTR 165 KT P YKY N + F+++YN V + + + FL+ + D + VD+ + R Sbjct: 146 KTDPSEYKYYLFNHLQFEIFYNKDRVIEIIVRTDQNFLVDLTEDKEVQVDFTYTVR 201 >At1g19930.1 68414.m02498 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 344 Score = 29.9 bits (64), Expect = 1.4 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = +3 Query: 345 KRPRRSLDSPRLDGYVLASSPIPHT 419 +RP + D+ + GYVLA+ PIPH+ Sbjct: 84 RRPILASDTRKSSGYVLATIPIPHS 108 >At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 383 Score = 28.7 bits (61), Expect = 3.2 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 8/50 (16%) Frame = +3 Query: 339 VDKRPRRSLDSPRLD-------GYVLASSPIPHTDWNEELKL-YAQSHGY 464 VD+ RS R+D GY ++ SP P W E +L + HGY Sbjct: 102 VDREADRSYKRARIDTIAGGSPGYGVSESPSPRISWENERRLKMVRDHGY 151 >At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 661 Score = 28.7 bits (61), Expect = 3.2 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 8/50 (16%) Frame = +3 Query: 339 VDKRPRRSLDSPRLD-------GYVLASSPIPHTDWNEELKL-YAQSHGY 464 VD+ RS R+D GY ++ SP P W E +L + HGY Sbjct: 102 VDREADRSYKRARIDTIAGGSPGYGVSESPSPRISWENERRLKMVRDHGY 151 >At3g12500.1 68416.m01556 basic endochitinase identical to basic endochitinase precursor SP:P19171 from [Arabidopsis thaliana] Length = 322 Score = 28.3 bits (60), Expect = 4.2 Identities = 11/46 (23%), Positives = 19/46 (41%) Frame = -3 Query: 460 PWLWAYSFNSSFQSVCGMGDDAKTYPSKRGESKERRGRLSTTWLFN 323 P+ W Y F + + T+P G+ RG + +W +N Sbjct: 150 PYSWGYCFKQEQNPASDYCEPSATWPCASGKRYYGRGPMQLSWNYN 195 >At5g10520.1 68418.m01218 protein kinase family protein contains protein kinase domain, INTERPRO:IPR000719 Length = 467 Score = 27.9 bits (59), Expect = 5.5 Identities = 14/47 (29%), Positives = 22/47 (46%) Frame = +2 Query: 206 CCSATADTIGPMAQSLFSCVQQIYSHAQHNSQTDRQLASIKKPGSRQ 346 C S ++D S FS + S + H Q ++ + SIKK R+ Sbjct: 49 CSSCSSDDKSSSTSSPFSNTTKTVSSSHHGLQWNKMIESIKKKSMRR 95 >At4g25710.1 68417.m03701 kelch repeat-containing F-box family protein similar to SKP1 interacting partner 6 [Arabidopsis thaliana] GI:10716957; contains Pfam profiles PF01344: Kelch motif, PF00646: F-box domain Length = 390 Score = 27.1 bits (57), Expect = 9.6 Identities = 12/28 (42%), Positives = 15/28 (53%) Frame = +3 Query: 366 DSPRLDGYVLASSPIPHTDWNEELKLYA 449 + R GYVLA PIPH+ + L A Sbjct: 112 EEDRSSGYVLARIPIPHSPLTQRYSLAA 139 >At2g16390.1 68415.m01876 SNF2 domain-containing protein / helicase domain-containing protein low similarity to RAD54 [Drosophila melanogaster] GI:1765914; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain Length = 888 Score = 27.1 bits (57), Expect = 9.6 Identities = 16/65 (24%), Positives = 29/65 (44%) Frame = +2 Query: 233 GPMAQSLFSCVQQIYSHAQHNSQTDRQLASIKKPGSRQTAAPFFRFSSFGRICFGIVAHT 412 G + +S+ + ++ A+ N++T K+ G +FS G + G+ AH Sbjct: 288 GVIEKSILEIIDVQFTKAKRNTRTYASETRTKRFGESDNE---LKFSEEGLMIGGLAAHP 344 Query: 413 THRLE 427 TH E Sbjct: 345 THAAE 349 >At1g62600.1 68414.m07062 flavin-containing monooxygenase family protein / FMO family protein low similarity to flavin-containing monooxygenase 2 from Cavia porcellus [SP|P36366]; contains Pfam profile PF00743 Flavin-binding monooxygenase-like Length = 452 Score = 27.1 bits (57), Expect = 9.6 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 2/45 (4%) Frame = +1 Query: 109 LIHIAPDLKN-SVDWN-GSTRKLLRVLNKRAYRQVLQCNGRYYWP 237 ++ +AP + S W ST K +VL Y V+ CNG Y P Sbjct: 137 VVKVAPAAEEGSGKWRIESTEKEKKVLRDEIYDAVVVCNGHYIEP 181 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,189,232 Number of Sequences: 28952 Number of extensions: 207804 Number of successful extensions: 611 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 594 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 611 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1206913392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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