BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120250.Seq (443 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 91 9e-20 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 91 9e-20 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 91 9e-20 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 91 9e-20 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 91 9e-20 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 62 5e-11 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 36 0.002 SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 33 0.026 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 27 0.98 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 26 3.0 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 25 5.2 SPAC13G6.06c |||glycine cleavage complex subunit P|Schizosacchar... 25 5.2 SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 6.9 SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces po... 25 6.9 SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharo... 25 6.9 SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 24 9.1 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 27 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 27 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 27 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 27 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 103 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 179 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 255 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 331 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 113 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 189 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 265 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 341 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 90.6 bits (215), Expect = 9e-20 Identities = 40/50 (80%), Positives = 47/50 (94%) Frame = +3 Query: 249 QAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 ++KI DKEG+P DQQRLIFAGKQLED +T++DYNIQKESTLH+VLRLRGG Sbjct: 27 KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 MQIF+KTLTGKTIT E E ++T+ ++K K+ K+ P Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 61.7 bits (143), Expect = 5e-11 Identities = 26/52 (50%), Positives = 37/52 (71%) Frame = +3 Query: 243 RSQAKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 398 R + ++ +KEG+P QQRLI+AGKQ+ D K Y+++ S LH+VL LRGG Sbjct: 25 RIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76 Score = 31.1 bits (67), Expect = 0.080 Identities = 14/37 (37%), Positives = 24/37 (64%) Frame = +1 Query: 172 MQIFIKTLTGKTITAETEPAETVADLKQKLPIKKVCP 282 M I +KTLTGK I + +P + V+ +K+++ K+ P Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIP 37 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 36.3 bits (80), Expect = 0.002 Identities = 16/44 (36%), Positives = 28/44 (63%) Frame = +3 Query: 258 IADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRL 389 IA + ++QRLI+AG+ L+D +++ Y IQ ++H+V L Sbjct: 33 IAPVADIEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLVKTL 76 >SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 32.7 bits (71), Expect = 0.026 Identities = 18/57 (31%), Positives = 31/57 (54%) Frame = -1 Query: 173 IFTLLLINDNGCCECALASTDGGIVQQAADTHTTNYFPSTAIDFHARNQKLSGRRAR 3 +F LLLI D+G + L +++ A DT+T +Y + +DF R +L G+ + Sbjct: 8 LFKLLLIGDSGVGKSCL------LLRFADDTYTESYISTIGVDFKIRTFELEGKTVK 58 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 27.5 bits (58), Expect = 0.98 Identities = 16/57 (28%), Positives = 30/57 (52%) Frame = -1 Query: 173 IFTLLLINDNGCCECALASTDGGIVQQAADTHTTNYFPSTAIDFHARNQKLSGRRAR 3 + LLLI D+G + L +++ + D+ T ++ + IDF R +L G+R + Sbjct: 9 LIKLLLIGDSGVGKSCL------LLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIK 59 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.8 bits (54), Expect = 3.0 Identities = 16/67 (23%), Positives = 31/67 (46%), Gaps = 2/67 (2%) Frame = +3 Query: 168 KNANIHQNIDGQNHYRRNGTRRDGGRSQAKIADKEGVPVDQQRLIF--AGKQLEDSKTMA 341 K+ N Q+I G+++ R+N GRS + D +R + K+ S + + Sbjct: 1518 KDKNKRQDISGESNSRQNNAISRAGRSNGSNRGNDSRDADGRRSTHYASNKRPRSSDSQS 1577 Query: 342 DYNIQKE 362 N+++E Sbjct: 1578 PSNLREE 1584 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 25.0 bits (52), Expect = 5.2 Identities = 11/36 (30%), Positives = 16/36 (44%) Frame = +3 Query: 183 HQNIDGQNHYRRNGTRRDGGRSQAKIADKEGVPVDQ 290 H + G + + G+RR GG +PVDQ Sbjct: 936 HGGMSGSHRGSQRGSRRGGGERDKPDPSSLTIPVDQ 971 >SPAC13G6.06c |||glycine cleavage complex subunit P|Schizosaccharomyces pombe|chr 1|||Manual Length = 1017 Score = 25.0 bits (52), Expect = 5.2 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = -1 Query: 338 HSFGIFQLFARKDKSLLIYGH 276 H FGIF + D S+ YGH Sbjct: 260 HVFGIFVQYPAADGSIFDYGH 280 >SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 475 Score = 24.6 bits (51), Expect = 6.9 Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = +2 Query: 203 KPLPPKRNPQRRWPISSKNCR*RRCA-RRSTKTYLCGQTIGRFQNYGRLQYSKGIYSSHG 379 KPL PK N + +W + N + R+S+++ G R GR +G YSSHG Sbjct: 135 KPLAPKANGKEKWVTITPNFTHTPISNRKSSRSRNDG---SRRNGNGR---RRGNYSSHG 188 >SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 451 Score = 24.6 bits (51), Expect = 6.9 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +1 Query: 100 TIPPSVEARAHSQQPLSFISNS 165 T+ PS RAHSQ P S + N+ Sbjct: 59 TMRPSALTRAHSQNPNSSLVNN 80 >SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 24.6 bits (51), Expect = 6.9 Identities = 11/44 (25%), Positives = 24/44 (54%) Frame = +3 Query: 255 KIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLR 386 +IAD D++++I+A + + + +AD+N H +L+ Sbjct: 542 EIADLRDELQDKEQIIYALRYAQKQRDIADFNQSLAKFPHKILK 585 >SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 2344 Score = 24.2 bits (50), Expect = 9.1 Identities = 9/28 (32%), Positives = 15/28 (53%) Frame = -1 Query: 122 ASTDGGIVQQAADTHTTNYFPSTAIDFH 39 A+ DG Q ++H NY+ +D+H Sbjct: 2238 AAEDGKETLQIFESHPDNYYQVCFVDYH 2265 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,888,441 Number of Sequences: 5004 Number of extensions: 39724 Number of successful extensions: 116 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 102 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 116 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 162176800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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