BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120240.Seq (882 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 31 1.0 At2g32780.1 68415.m04013 ubiquitin-specific protease 1, putative... 29 4.1 At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 5.4 At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 5.4 At5g03560.1 68418.m00314 expressed protein 28 7.2 At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 28 7.2 At5g52920.1 68418.m06567 pyruvate kinase, putative similar to py... 28 9.5 At5g08780.1 68418.m01041 histone H1/H5 family protein contains P... 28 9.5 At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 28 9.5 At4g25320.1 68417.m03643 DNA-binding protein-related contains Pf... 28 9.5 At1g77060.1 68414.m08972 mutase family protein similar to carbox... 28 9.5 At1g70420.1 68414.m08102 expressed protein 28 9.5 >At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative similar to GTL1 [Arabidopsis thaliana] GI:2664198 Length = 669 Score = 31.1 bits (67), Expect = 1.0 Identities = 21/47 (44%), Positives = 26/47 (55%), Gaps = 2/47 (4%) Frame = -3 Query: 646 RHAVGQLRRLRRAFSQSCRAVSASRRATIISLANKMSDRL--LPPNL 512 R + +L R SQ RA SASR A IISL K++ LPP+L Sbjct: 308 RQEMARLAREHEVMSQE-RAASASRDAAIISLIQKITGHTIQLPPSL 353 >At2g32780.1 68415.m04013 ubiquitin-specific protease 1, putative (UBP1) similar to GI:11993461 Length = 1083 Score = 29.1 bits (62), Expect = 4.1 Identities = 20/63 (31%), Positives = 27/63 (42%), Gaps = 3/63 (4%) Frame = +1 Query: 505 GHADLAEATDRSFCLPTK*LWLDATLKRLGKIAKTRAANGAA---GQPHGGHCARRDSQT 675 GH D E D S + T+ D + RL + + A GGH RRDS T Sbjct: 987 GHVDFQEFIDLSKYMDTRCSEEDEPVYRLAGLVEHLGAMSRGHYVSYIRGGHKERRDSDT 1046 Query: 676 QQP 684 ++P Sbjct: 1047 KEP 1049 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 28.7 bits (61), Expect = 5.4 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +3 Query: 57 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 197 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 822 Score = 28.7 bits (61), Expect = 5.4 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +3 Query: 57 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 197 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g03560.1 68418.m00314 expressed protein Length = 241 Score = 28.3 bits (60), Expect = 7.2 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = -3 Query: 556 SLANKMSDRLLPPNLRGHRQFLR 488 ++ + + DR LPP LRG R F+R Sbjct: 81 AVIDAVHDRKLPPELRGRRDFVR 103 >At2g02790.1 68415.m00222 calmodulin-binding family protein very low similarity to SP|P12036 Neurofilament triplet H protein {Homo sapiens}; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 636 Score = 28.3 bits (60), Expect = 7.2 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%) Frame = +1 Query: 184 NGQYVSTSTASTNRRLSMPTKSSTCSR*RG--KAGR--PAVFAPTHSAHYQI 327 NG S +T ++RR S+P K + G ++GR P+ APT SA +I Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523 >At5g52920.1 68418.m06567 pyruvate kinase, putative similar to pyruvate kinase isozyme G, chloroplast precursor [Nicotiana tabacum] SWISS-PROT:Q40546 Length = 579 Score = 27.9 bits (59), Expect = 9.5 Identities = 18/91 (19%), Positives = 40/91 (43%), Gaps = 2/91 (2%) Frame = +1 Query: 589 LGKIAKTRAANGAAGQPHGGHCARRDSQTQQPAAXXXXXXXXXXXXXXCVSAPAKTIAGR 768 + ++ TR+ G+ P+GG + R + +PA+ VS ++ + Sbjct: 1 MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60 Query: 769 SLK--RLGSNDVIFSSDYVPNSMNVLNKVKE 855 +++ R+ + + S + VPN L ++ E Sbjct: 61 TVRSARVETEVIPVSPEDVPNREEQLERLLE 91 >At5g08780.1 68418.m01041 histone H1/H5 family protein contains Pfam domain, PF00538: linker histone H1 and H5 family Length = 457 Score = 27.9 bits (59), Expect = 9.5 Identities = 16/48 (33%), Positives = 22/48 (45%), Gaps = 1/48 (2%) Frame = +3 Query: 183 ERAIRVHVDGKYKST-FEHADQIQHMLQIAWQSRATRCICTHTQCSLP 323 E AI + KYK+ F H + + H L + R C C + SLP Sbjct: 77 EDAISEFIKSKYKNLPFAHTNLLSHHLAKLVEKREILCDCNNDCYSLP 124 >At4g37060.1 68417.m05248 patatin, putative similar to patatin-like latex allergen [Hevea brasiliensis][PMID:10589016]; contains patatin domain PF01734 Length = 414 Score = 27.9 bits (59), Expect = 9.5 Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 2/66 (3%) Frame = +2 Query: 272 AKQGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLLEEVIPQV-LCTGKYAPAV 445 A Q + YL L + + L KS L I+L E +L + Q+ + TG Y PA Sbjct: 313 ALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKMLTNRVMQMNIDTGTYEPAA 372 Query: 446 EMDTND 463 E ND Sbjct: 373 ENINND 378 >At4g25320.1 68417.m03643 DNA-binding protein-related contains Pfam domain PF03479: Domain of unknown function (DUF296), found in AT-hook motifs Pfam:PF02178 Length = 404 Score = 27.9 bits (59), Expect = 9.5 Identities = 12/34 (35%), Positives = 21/34 (61%) Frame = +1 Query: 202 TSTASTNRRLSMPTKSSTCSR*RGKAGRPAVFAP 303 T A+T L+MPT++++ + + K GRP + P Sbjct: 64 TENAATPFSLTMPTENTSAEQLKKKRGRPRKYNP 97 >At1g77060.1 68414.m08972 mutase family protein similar to carboxyvinyl-carboxyphosphonate phosphorylmutase GB:O49290 from [Arabidopsis thaliana]; similar to carboxyphosphonoenolpyruvate mutase (GI:47149) [Streptomyces hygroscopicus]; contains Prosite PS00161: Isocitrate lyase signature Length = 339 Score = 27.9 bits (59), Expect = 9.5 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +2 Query: 248 PAHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKS 355 P H PD + + G L +HP T L + + ++K+ Sbjct: 256 PLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKT 291 >At1g70420.1 68414.m08102 expressed protein Length = 272 Score = 27.9 bits (59), Expect = 9.5 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +1 Query: 184 NGQYVSTSTASTNRRLSMPTKSSTCSR*RGKAGRPAVFAPTHSAHYQIW 330 NG ++ST+ST RLS KSS + + K + T SAH +++ Sbjct: 187 NGSSSTSSTSSTAARLSGVVKSSEKGKEKTKTDKKKDKMRTKSAHEKLY 235 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,144,284 Number of Sequences: 28952 Number of extensions: 410958 Number of successful extensions: 1162 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 1130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1161 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 2077687200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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