BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120231.Seq (864 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos... 111 2e-25 SPBC336.10c |tif512||translation initiation factor|Schizosacchar... 110 2e-25 SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 3.4 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 4.5 SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|ch... 27 4.5 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 6.0 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 26 7.9 >SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 111 bits (266), Expect = 2e-25 Identities = 47/85 (55%), Positives = 67/85 (78%) Frame = +1 Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGT 435 IFNG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM +G ++D+++P+G+LG Sbjct: 65 IFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGN 123 Query: 436 QLRTDFDSGKELLCTVLKSCGEECA 510 ++ FD G++L+ TV+ + GEE A Sbjct: 124 EIEEGFDEGRDLIITVVSAMGEETA 148 Score = 104 bits (250), Expect = 1e-23 Identities = 46/61 (75%), Positives = 53/61 (86%) Frame = +2 Query: 65 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 244 M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+ Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60 Query: 245 V 247 V Sbjct: 61 V 61 >SPBC336.10c |tif512||translation initiation factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 110 bits (265), Expect = 2e-25 Identities = 47/85 (55%), Positives = 67/85 (78%) Frame = +1 Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGT 435 IFNG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM +G ++D+++P+G+LG Sbjct: 65 IFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGN 123 Query: 436 QLRTDFDSGKELLCTVLKSCGEECA 510 ++ F+ GK+L+ TV+ + GEE A Sbjct: 124 EIEEGFEEGKDLIITVVSAMGEEIA 148 Score = 104 bits (250), Expect = 1e-23 Identities = 46/61 (75%), Positives = 53/61 (86%) Frame = +2 Query: 65 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 244 M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+ Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60 Query: 245 V 247 V Sbjct: 61 V 61 >SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 762 Score = 27.1 bits (57), Expect = 3.4 Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 1/55 (1%) Frame = +1 Query: 400 EDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCG-EECASPSKQTQLSTNKPTQHL 561 EDL + D + G+ + + FD LLC+V+ CA P K + + +L Sbjct: 95 EDLLLEDTEDGSIVVSKFDRISNLLCSVIDLFEFVRCAHPDKMVVMKAEEAYSYL 149 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 26.6 bits (56), Expect = 4.5 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = +1 Query: 301 NMDVPHVKREDYQLTDISDDGYL 369 NM +P+++ E Q+ D + D YL Sbjct: 968 NMSIPYIRGEYVQMIDANQDNYL 990 >SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 204 Score = 26.6 bits (56), Expect = 4.5 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 157 FRYAEGSSMQDC*NVHIQNRKARPR*SSLGWD 252 + + E +Q C N+ +N +A R SS GWD Sbjct: 41 YHHLEKGLLQQCFNLVKKNMEALYRQSSFGWD 72 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 26.2 bits (55), Expect = 6.0 Identities = 12/32 (37%), Positives = 15/32 (46%) Frame = -3 Query: 400 HAGHRCQPSGSGSHHPRYQSVGSLRASRGVRP 305 H G P+ PR + GS A+RG RP Sbjct: 957 HVGTGLPPTSKSKPFPRLATGGSTAAARGPRP 988 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 25.8 bits (54), Expect = 7.9 Identities = 17/76 (22%), Positives = 32/76 (42%) Frame = +3 Query: 3 SVEEPVGHISFLTVVKFKTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVH 182 S E+P+ +S L+ TS + P P P PP+ + P + + ++ + Sbjct: 222 STEKPLLSVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTV--KTTT 279 Query: 183 ARLLKCPHPKPESTAT 230 + + P P S +T Sbjct: 280 SNASRQPSPLVSSKST 295 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,572,503 Number of Sequences: 5004 Number of extensions: 74901 Number of successful extensions: 222 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 207 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 220 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 430470850 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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