SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120231.Seq
         (864 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos...   111   2e-25
SPBC336.10c |tif512||translation initiation factor|Schizosacchar...   110   2e-25
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc...    27   3.4  
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic...    27   4.5  
SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|ch...    27   4.5  
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2...    26   6.0  
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc...    26   7.9  

>SPAC26H5.10c |tif51||translation initiation factor
           eIF5A|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score =  111 bits (266), Expect = 2e-25
 Identities = 47/85 (55%), Positives = 67/85 (78%)
 Frame = +1

Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGT 435
           IFNG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM  +G  ++D+++P+G+LG 
Sbjct: 65  IFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGN 123

Query: 436 QLRTDFDSGKELLCTVLKSCGEECA 510
           ++   FD G++L+ TV+ + GEE A
Sbjct: 124 EIEEGFDEGRDLIITVVSAMGEETA 148



 Score =  104 bits (250), Expect = 1e-23
 Identities = 46/61 (75%), Positives = 53/61 (86%)
 Frame = +2

Query: 65  MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 244
           M + E   FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+
Sbjct: 1   MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60

Query: 245 V 247
           V
Sbjct: 61  V 61


>SPBC336.10c |tif512||translation initiation
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 157

 Score =  110 bits (265), Expect = 2e-25
 Identities = 47/85 (55%), Positives = 67/85 (78%)
 Frame = +1

Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGT 435
           IFNG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM  +G  ++D+++P+G+LG 
Sbjct: 65  IFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGN 123

Query: 436 QLRTDFDSGKELLCTVLKSCGEECA 510
           ++   F+ GK+L+ TV+ + GEE A
Sbjct: 124 EIEEGFEEGKDLIITVVSAMGEEIA 148



 Score =  104 bits (250), Expect = 1e-23
 Identities = 46/61 (75%), Positives = 53/61 (86%)
 Frame = +2

Query: 65  MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 244
           M + E   FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+
Sbjct: 1   MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60

Query: 245 V 247
           V
Sbjct: 61  V 61


>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 762

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = +1

Query: 400 EDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCG-EECASPSKQTQLSTNKPTQHL 561
           EDL + D + G+ + + FD    LLC+V+       CA P K   +   +   +L
Sbjct: 95  EDLLLEDTEDGSIVVSKFDRISNLLCSVIDLFEFVRCAHPDKMVVMKAEEAYSYL 149


>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
            subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1729

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = +1

Query: 301  NMDVPHVKREDYQLTDISDDGYL 369
            NM +P+++ E  Q+ D + D YL
Sbjct: 968  NMSIPYIRGEYVQMIDANQDNYL 990


>SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 204

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +1

Query: 157 FRYAEGSSMQDC*NVHIQNRKARPR*SSLGWD 252
           + + E   +Q C N+  +N +A  R SS GWD
Sbjct: 41  YHHLEKGLLQQCFNLVKKNMEALYRQSSFGWD 72


>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1217

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 12/32 (37%), Positives = 15/32 (46%)
 Frame = -3

Query: 400  HAGHRCQPSGSGSHHPRYQSVGSLRASRGVRP 305
            H G    P+      PR  + GS  A+RG RP
Sbjct: 957  HVGTGLPPTSKSKPFPRLATGGSTAAARGPRP 988


>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
           Shk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 658

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 17/76 (22%), Positives = 32/76 (42%)
 Frame = +3

Query: 3   SVEEPVGHISFLTVVKFKTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVH 182
           S E+P+  +S L+          TS +    P  P P PP+  +  P + + ++  +   
Sbjct: 222 STEKPLLSVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTV--KTTT 279

Query: 183 ARLLKCPHPKPESTAT 230
           +   + P P   S +T
Sbjct: 280 SNASRQPSPLVSSKST 295


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,572,503
Number of Sequences: 5004
Number of extensions: 74901
Number of successful extensions: 222
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -