BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120231.Seq (864 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 106 2e-23 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 103 2e-22 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 102 4e-22 At3g06450.1 68416.m00746 anion exchange family protein similar t... 32 0.57 At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 29 3.0 At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 4.0 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 5.3 At5g13880.1 68418.m01622 expressed protein 29 5.3 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 5.3 At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 29 5.3 At5g05810.1 68418.m00639 zinc finger (C3HC4-type RING finger) fa... 28 9.2 At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 9.2 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 9.2 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 106 bits (255), Expect = 2e-23 Identities = 47/84 (55%), Positives = 64/84 (76%), Gaps = 1/84 (1%) Frame = +1 Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 432 IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123 Query: 433 TQLRTDFDSGKELLCTVLKSCGEE 504 Q+++ FD GK+L+ +V+ + GEE Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEE 147 Score = 91.9 bits (218), Expect = 5e-19 Identities = 36/57 (63%), Positives = 47/57 (82%) Frame = +2 Query: 77 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLV 247 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFV 60 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 103 bits (247), Expect = 2e-22 Identities = 46/84 (54%), Positives = 63/84 (75%), Gaps = 1/84 (1%) Frame = +1 Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 432 IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123 Query: 433 TQLRTDFDSGKELLCTVLKSCGEE 504 TQL+ F+ GK+++ +V+ + GEE Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEE 147 Score = 88.2 bits (209), Expect = 6e-18 Identities = 35/57 (61%), Positives = 46/57 (80%) Frame = +2 Query: 77 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLV 247 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFV 60 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 102 bits (244), Expect = 4e-22 Identities = 45/84 (53%), Positives = 62/84 (73%), Gaps = 1/84 (1%) Frame = +1 Query: 256 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 432 IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123 Query: 433 TQLRTDFDSGKELLCTVLKSCGEE 504 Q+R FD GK+++ +V+ S GEE Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEE 147 Score = 83.0 bits (196), Expect = 2e-16 Identities = 33/57 (57%), Positives = 43/57 (75%) Frame = +2 Query: 77 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLV 247 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFV 60 >At3g06450.1 68416.m00746 anion exchange family protein similar to putative Anion exchanger family members: GB:AAD39673, GB:AAD55295 [Arabidopsis thaliana] Length = 732 Score = 31.9 bits (69), Expect = 0.57 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = -3 Query: 718 IASDVSEGEAPRGLRAPPLYYYSFFKLNLYTILC 617 +AS +++ E LR PP Y+Y F L TILC Sbjct: 316 VASQLAQQE-DFNLRKPPAYHYDLFLLGFLTILC 348 >At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-related contains weak similarity to Calcium-binding mitochondrial protein Anon-60Da (Swiss-Prot:P91927) [Drosophila melanogaster] Length = 755 Score = 29.5 bits (63), Expect = 3.0 Identities = 17/44 (38%), Positives = 22/44 (50%) Frame = +3 Query: 135 VRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLYLQW 266 + P +K V+ R A+ K H K E +TLK WLG L W Sbjct: 182 IGPAIKAVASMNRADWAK--KLTHWKHEFVSTLKHYWLGTKLLW 223 >At5g14230.1 68418.m01663 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 591 Score = 29.1 bits (62), Expect = 4.0 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +1 Query: 406 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 507 L IPDGD T L G +C L SCG C Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 28.7 bits (61), Expect = 5.3 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +3 Query: 42 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 128 ++ F T Q K++ S P +P P PP P Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108 >At5g13880.1 68418.m01622 expressed protein Length = 188 Score = 28.7 bits (61), Expect = 5.3 Identities = 9/36 (25%), Positives = 19/36 (52%) Frame = +3 Query: 6 VEEPVGHISFLTVVKFKTQQWVTSKTHTSRPETPGP 113 +E+ + ++ L + +F Q W+ + P+TP P Sbjct: 150 LEDEIDKVADLFITRFHRQMWLQKQLSLESPDTPSP 185 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 5.3 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -1 Query: 177 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 70 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At2g27680.1 68415.m03354 aldo/keto reductase family protein contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family Length = 384 Score = 28.7 bits (61), Expect = 5.3 Identities = 26/95 (27%), Positives = 44/95 (46%) Frame = +1 Query: 277 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFD 456 ++I S MDV + + D ++DGYL + DL+E +G + T T+FD Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKE-----EGKIKTVALTNFD 201 Query: 457 SGKELLCTVLKSCGEECASPSKQTQLSTNKPTQHL 561 + E L +L++ G S Q + +P Q + Sbjct: 202 T--ERLQKILEN-GIPVVSNQVQHSIVDMRPQQRM 233 >At5g05810.1 68418.m00639 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 353 Score = 27.9 bits (59), Expect = 9.2 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = -2 Query: 779 LSITELGRDYGTRRRIEGVRYCE*RQRGGGAPRSPSTTSVL 657 L+ E GRD R + + E R+R GG PR T+V+ Sbjct: 297 LAAAEGGRDVINGRSVSELTGIERRRRWGGEPRQRQATAVI 337 >At3g53330.1 68416.m05884 plastocyanin-like domain-containing protein similar to mavicyanin SP:P80728 from [Cucurbita pepo] Length = 310 Score = 27.9 bits (59), Expect = 9.2 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%) Frame = +3 Query: 75 SKTHT-SRPETPGPQPPS 125 SKTH SRP TP P PPS Sbjct: 127 SKTHERSRPITPSPPPPS 144 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.9 bits (59), Expect = 9.2 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%) Frame = +3 Query: 78 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 203 KT T P PP PCN PC S + + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,945,861 Number of Sequences: 28952 Number of extensions: 416011 Number of successful extensions: 1177 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 1075 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1163 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 2019160800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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