BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120230X.Seq
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26340.1 68415.m03160 expressed protein 29 2.5
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 28 3.2
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 28 3.2
At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 27 5.7
At1g79640.1 68414.m09286 protein kinase family protein contains ... 27 9.9
At1g77060.1 68414.m08972 mutase family protein similar to carbox... 27 9.9
>At2g26340.1 68415.m03160 expressed protein
Length = 230
Score = 28.7 bits (61), Expect = 2.5
Identities = 20/54 (37%), Positives = 27/54 (50%)
Frame = +2
Query: 329 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTSDVIARIDDL 490
S ++MKS+L A+ + LL+ V QVL GK V S+ A DDL
Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = +3
Query: 60 QVKIGQFKFGQDEFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200
Q G F +D T +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARD--THQYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = +3
Query: 60 QVKIGQFKFGQDEFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200
Q G F +D T +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARD--THQYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At2g02790.1 68415.m00222 calmodulin-binding family protein very low
similarity to SP|P12036 Neurofilament triplet H protein
{Homo sapiens}; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 636
Score = 27.5 bits (58), Expect = 5.7
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Frame = +1
Query: 187 NGQYVSTSTASTNRRLSMPTKSRPCSR*RG--KAGR--PAVFAPTHSAHYQI 330
NG S +T ++RR S+P K + G ++GR P+ APT SA +I
Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523
>At1g79640.1 68414.m09286 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 694
Score = 26.6 bits (56), Expect = 9.9
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 5/75 (6%)
Frame = +3
Query: 120 GDEQPVRFVAKD--IASSLKYVNCE-RAIRVHVDGKYKSTFEHAD--QIQTMLQIAWQSR 284
GD+ P K ASSL + + + V G++K T E+ D ++ I +S
Sbjct: 511 GDDVPTEMAVKPPKAASSLDESDDKSKPPVVQQRGRFKVTSENLDIEKVVAPSPILQKSH 570
Query: 285 ATRCICTHTQCSLPN 329
+ + +C H+ SLP+
Sbjct: 571 SMQVLCQHSSASLPH 585
>At1g77060.1 68414.m08972 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 339
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +2
Query: 251 PDHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKS 358
P H PD + + G L +HP T L + + ++K+
Sbjct: 256 PLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKT 291
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,869,630
Number of Sequences: 28952
Number of extensions: 262015
Number of successful extensions: 674
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 665
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 674
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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