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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120230X.Seq
         (516 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g26340.1 68415.m03160 expressed protein                             29   2.5  
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain...    28   3.2  
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain...    28   3.2  
At2g02790.1 68415.m00222 calmodulin-binding family protein very ...    27   5.7  
At1g79640.1 68414.m09286 protein kinase family protein contains ...    27   9.9  
At1g77060.1 68414.m08972 mutase family protein similar to carbox...    27   9.9  

>At2g26340.1 68415.m03160 expressed protein
          Length = 230

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 20/54 (37%), Positives = 27/54 (50%)
 Frame = +2

Query: 329 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTSDVIARIDDL 490
           S    ++MKS+L  A+   + LL+ V  QVL  GK    V    S+  A  DDL
Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218


>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 870

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 17/47 (36%), Positives = 23/47 (48%)
 Frame = +3

Query: 60  QVKIGQFKFGQDEFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200
           Q   G F   +D  T +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARD--THQYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 822

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 17/47 (36%), Positives = 23/47 (48%)
 Frame = +3

Query: 60  QVKIGQFKFGQDEFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200
           Q   G F   +D  T +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARD--THQYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At2g02790.1 68415.m00222 calmodulin-binding family protein very low
           similarity to SP|P12036 Neurofilament triplet H protein
           {Homo sapiens}; contains Pfam profile PF00612: IQ
           calmodulin-binding motif
          Length = 636

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
 Frame = +1

Query: 187 NGQYVSTSTASTNRRLSMPTKSRPCSR*RG--KAGR--PAVFAPTHSAHYQI 330
           NG   S +T  ++RR S+P K     +  G  ++GR  P+  APT SA  +I
Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523


>At1g79640.1 68414.m09286 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 694

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 5/75 (6%)
 Frame = +3

Query: 120 GDEQPVRFVAKD--IASSLKYVNCE-RAIRVHVDGKYKSTFEHAD--QIQTMLQIAWQSR 284
           GD+ P     K    ASSL   + + +   V   G++K T E+ D  ++     I  +S 
Sbjct: 511 GDDVPTEMAVKPPKAASSLDESDDKSKPPVVQQRGRFKVTSENLDIEKVVAPSPILQKSH 570

Query: 285 ATRCICTHTQCSLPN 329
           + + +C H+  SLP+
Sbjct: 571 SMQVLCQHSSASLPH 585


>At1g77060.1 68414.m08972 mutase family protein similar to
           carboxyvinyl-carboxyphosphonate phosphorylmutase
           GB:O49290 from [Arabidopsis thaliana]; similar to
           carboxyphosphonoenolpyruvate mutase (GI:47149)
           [Streptomyces hygroscopicus]; contains Prosite PS00161:
           Isocitrate lyase signature
          Length = 339

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +2

Query: 251 PDHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKS 358
           P H PD + + G  L +HP T L   +  +  ++K+
Sbjct: 256 PLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKT 291


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,869,630
Number of Sequences: 28952
Number of extensions: 262015
Number of successful extensions: 674
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 665
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 674
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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