BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120230X.Seq (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g26340.1 68415.m03160 expressed protein 29 2.5 At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 28 3.2 At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 28 3.2 At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 27 5.7 At1g79640.1 68414.m09286 protein kinase family protein contains ... 27 9.9 At1g77060.1 68414.m08972 mutase family protein similar to carbox... 27 9.9 >At2g26340.1 68415.m03160 expressed protein Length = 230 Score = 28.7 bits (61), Expect = 2.5 Identities = 20/54 (37%), Positives = 27/54 (50%) Frame = +2 Query: 329 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTSDVIARIDDL 490 S ++MKS+L A+ + LL+ V QVL GK V S+ A DDL Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 28.3 bits (60), Expect = 3.2 Identities = 17/47 (36%), Positives = 23/47 (48%) Frame = +3 Query: 60 QVKIGQFKFGQDEFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200 Q G F +D T +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARD--THQYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 822 Score = 28.3 bits (60), Expect = 3.2 Identities = 17/47 (36%), Positives = 23/47 (48%) Frame = +3 Query: 60 QVKIGQFKFGQDEFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200 Q G F +D T +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARD--THQYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At2g02790.1 68415.m00222 calmodulin-binding family protein very low similarity to SP|P12036 Neurofilament triplet H protein {Homo sapiens}; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 636 Score = 27.5 bits (58), Expect = 5.7 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%) Frame = +1 Query: 187 NGQYVSTSTASTNRRLSMPTKSRPCSR*RG--KAGR--PAVFAPTHSAHYQI 330 NG S +T ++RR S+P K + G ++GR P+ APT SA +I Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523 >At1g79640.1 68414.m09286 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 694 Score = 26.6 bits (56), Expect = 9.9 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 5/75 (6%) Frame = +3 Query: 120 GDEQPVRFVAKD--IASSLKYVNCE-RAIRVHVDGKYKSTFEHAD--QIQTMLQIAWQSR 284 GD+ P K ASSL + + + V G++K T E+ D ++ I +S Sbjct: 511 GDDVPTEMAVKPPKAASSLDESDDKSKPPVVQQRGRFKVTSENLDIEKVVAPSPILQKSH 570 Query: 285 ATRCICTHTQCSLPN 329 + + +C H+ SLP+ Sbjct: 571 SMQVLCQHSSASLPH 585 >At1g77060.1 68414.m08972 mutase family protein similar to carboxyvinyl-carboxyphosphonate phosphorylmutase GB:O49290 from [Arabidopsis thaliana]; similar to carboxyphosphonoenolpyruvate mutase (GI:47149) [Streptomyces hygroscopicus]; contains Prosite PS00161: Isocitrate lyase signature Length = 339 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +2 Query: 251 PDHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKS 358 P H PD + + G L +HP T L + + ++K+ Sbjct: 256 PLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKT 291 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,869,630 Number of Sequences: 28952 Number of extensions: 262015 Number of successful extensions: 674 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 665 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 674 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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