BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120215.Seq (831 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.46 SPBC24C6.10c |||conserved eukaryotic protein|Schizosaccharomyces... 28 1.4 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 2.5 SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schi... 27 2.5 SPBC16D10.02 |trm11||tRNA |Schizosaccharomyces pombe|chr 2|||Manual 27 3.3 SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomy... 27 4.3 SPAC8F11.05c |mug130||sequence orphan|Schizosaccharomyces pombe|... 26 7.5 SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 7.5 SPBC18H10.21c ||SPBC9B6.01c|dubious|Schizosaccharomyces pombe|ch... 26 7.5 SPBC428.05c |arg12||argininosuccinate synthase |Schizosaccharomy... 25 10.0 SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 25 10.0 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 29.9 bits (64), Expect = 0.46 Identities = 16/69 (23%), Positives = 32/69 (46%) Frame = +1 Query: 556 AFIHNRFHPLVTNFTNKMEFVVTETNDTSIPGEPILFTENEGVLLCSVDRPSIVKMLSRE 735 AF H F ++ + N +E N + P+L T+ L +D P ++++ + Sbjct: 1380 AFDHQSFKDIIVHVAN-LELYYRALN-FYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKS 1437 Query: 736 FDTEALVNF 762 +T ++NF Sbjct: 1438 ENTPLILNF 1446 >SPBC24C6.10c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 28.3 bits (60), Expect = 1.4 Identities = 16/36 (44%), Positives = 22/36 (61%), Gaps = 2/36 (5%) Frame = -3 Query: 367 GFLDLFI-AVYNFGDRSNFVAFDTFHF-YTLIYNIC 266 GF+ + I A+ F DRS VAF +F + L+Y IC Sbjct: 120 GFIPVLIKAMKQFKDRSENVAFTSFRYALFLVYYIC 155 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 27.5 bits (58), Expect = 2.5 Identities = 21/76 (27%), Positives = 39/76 (51%), Gaps = 5/76 (6%) Frame = -2 Query: 755 TKASVSNSRLSILTIDGLSTEHNSTPSFSVNKMGSPGMLVSLVSVTTN--SILLVKLVTS 582 +++SV + I + GLS ++ + + + +G+P +S VSVT N ++ + T+ Sbjct: 182 SQSSVVSVNSEIFSYFGLSQQYVNYSTSRLCVVGTPRANMSTVSVTNNGSAVSNYTVNTN 241 Query: 581 GW---NLLCIKANVLN 543 GW N C+ V N Sbjct: 242 GWTSSNFKCVDDVVAN 257 >SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 680 Score = 27.5 bits (58), Expect = 2.5 Identities = 19/71 (26%), Positives = 34/71 (47%) Frame = -1 Query: 480 ISRVLSIAMYLLSANLIFRVS*LSKLLRFFKSNSANS*VFWIFSSLFTISVTVRTLLHLT 301 + R+LSIA +LLS + + R+ + + N+ S V + T SV V+ L + Sbjct: 14 LKRILSIAFFLLSLSTLLRIVNAQQ----YVDNNIGSMVLSDYDFAETPSVRVQRALEIL 69 Query: 300 RFIFIHLSTTF 268 R+ + T+ Sbjct: 70 RYYYEQEDVTY 80 >SPBC16D10.02 |trm11||tRNA |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 27.1 bits (57), Expect = 3.3 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = +1 Query: 439 GAQQIHGYAQHPGNDPAAVGNI*NKADTRQIAAVVFSTLAFIHNR 573 GA++I +Q P + ++ GN K + QI+ +V+ + F +R Sbjct: 326 GAKKIAKCSQRPPKESSSTGNHYPKLEQYQISDMVYDIICFASDR 370 >SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 4.3 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -1 Query: 648 GNACVISFSDHKLHFVSKISNK 583 G+AC +S + K+H ++ ISN+ Sbjct: 230 GSACAVSDDEGKIHVINDISNE 251 >SPAC8F11.05c |mug130||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 241 Score = 25.8 bits (54), Expect = 7.5 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 3/32 (9%) Frame = +1 Query: 556 AFIHNRFHPLV---TNFTNKMEFVVTETNDTS 642 AFI N++ PLV T++TN V +TN S Sbjct: 112 AFIENKYSPLVFHATSWTNGFSIDVQKTNPIS 143 >SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosaccharomyces pombe|chr 2|||Manual Length = 297 Score = 25.8 bits (54), Expect = 7.5 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -1 Query: 651 SGNACVISFSDHKLHFVSKISNKW 580 SGN +S D+K++ + NKW Sbjct: 266 SGNILAVSCGDNKVYLFKESQNKW 289 >SPBC18H10.21c ||SPBC9B6.01c|dubious|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.8 bits (54), Expect = 7.5 Identities = 11/21 (52%), Positives = 12/21 (57%) Frame = +3 Query: 48 NSKHVFDHFGCSSNYCFNNYV 110 N VF+H CS Y FNN V Sbjct: 2 NGLRVFEHVHCSVLYKFNNIV 22 >SPBC428.05c |arg12||argininosuccinate synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 410 Score = 25.4 bits (53), Expect = 10.0 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = -2 Query: 611 SILLVKLVTSGWNLLCIKANV 549 S +L L+ GW ++C ANV Sbjct: 19 SCILAWLIEEGWEVICYMANV 39 >SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 786 Score = 25.4 bits (53), Expect = 10.0 Identities = 21/70 (30%), Positives = 30/70 (42%) Frame = -2 Query: 809 RGAKRLGYPHVAVIVLKFTKASVSNSRLSILTIDGLSTEHNSTPSFSVNKMGSPGMLVSL 630 +GAKR+ + VA L +ILT+DG T SVN + G V++ Sbjct: 3 QGAKRVRFYIVAADGLSKRDLFRQPDPFAILTVDGEQTHTTKVIKKSVNPYWNEGFEVTV 62 Query: 629 VSVTTNSILL 600 + SI L Sbjct: 63 KPSSVISIRL 72 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,045,475 Number of Sequences: 5004 Number of extensions: 57398 Number of successful extensions: 173 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 173 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 408446760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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