Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120174.Seq
(817 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79640.1 68414.m09286 protein kinase family protein contains ... 30 1.6
At5g08780.1 68418.m01041 histone H1/H5 family protein contains P... 30 2.1
At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 29 2.8
At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein ... 29 3.7
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 4.9
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 4.9
At5g43490.1 68418.m05317 hypothetical protein similar to unknown... 28 6.4
At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 28 8.5
At4g25320.1 68417.m03643 DNA-binding protein-related contains Pf... 28 8.5
At1g09970.2 68414.m01124 leucine-rich repeat transmembrane prote... 28 8.5
At1g09970.1 68414.m01123 leucine-rich repeat transmembrane prote... 28 8.5
>At1g79640.1 68414.m09286 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 694
Score = 30.3 bits (65), Expect = 1.6
Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 5/75 (6%)
Frame = +3
Query: 108 GDEQPVRFVAKD--IASSLKYVNCE-RAIRVHVDGKYKSTFEHAD--QIQHHAPIAWQSR 272
GD+ P K ASSL + + + V G++K T E+ D ++ +PI +S
Sbjct: 511 GDDVPTEMAVKPPKAASSLDESDDKSKPPVVQQRGRFKVTSENLDIEKVVAPSPILQKSH 570
Query: 273 ATRCICTHTQCSLPN 317
+ + +C H+ SLP+
Sbjct: 571 SMQVLCQHSSASLPH 585
>At5g08780.1 68418.m01041 histone H1/H5 family protein contains Pfam
domain, PF00538: linker histone H1 and H5 family
Length = 457
Score = 29.9 bits (64), Expect = 2.1
Identities = 16/48 (33%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +3
Query: 174 ERAIRVHVDGKYKST-FEHADQIQHHAPIAWQSRATRCICTHTQCSLP 314
E AI + KYK+ F H + + HH + R C C + SLP
Sbjct: 77 EDAISEFIKSKYKNLPFAHTNLLSHHLAKLVEKREILCDCNNDCYSLP 124
>At2g02790.1 68415.m00222 calmodulin-binding family protein very low
similarity to SP|P12036 Neurofilament triplet H protein
{Homo sapiens}; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 636
Score = 29.5 bits (63), Expect = 2.8
Identities = 20/52 (38%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Frame = +1
Query: 175 NGQYVSTSTASTNRRLSMPTKSSTMLQ*RG--KAGR--PAVFAPTHSAHYQI 318
NG S +T ++RR S+P K Q G ++GR P+ APT SA +I
Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523
>At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein
contains Pfam PF00096: Zinc finger, C2H2 type
Length = 387
Score = 29.1 bits (62), Expect = 3.7
Identities = 17/77 (22%), Positives = 35/77 (45%)
Frame = +2
Query: 335 IMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDLTQKLTVANADWR 514
I+KSK P ++EW+ ++++ TG + T D +++ + ++ V +
Sbjct: 17 ILKSKAPAVKWIKEWVPQDIV----ATGGKCHLHKWVTEDTFSRLKEKEKEPDVPEPEPE 72
Query: 515 KQPIAHFVCQRNDCGST 565
F+C + CG T
Sbjct: 73 PTTEILFLCSYDGCGKT 89
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +3
Query: 48 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 188
Q G F DT +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +3
Query: 48 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 188
Q G F DT +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At5g43490.1 68418.m05317 hypothetical protein similar to unknown
protein (gb|AAF20218.1)
Length = 131
Score = 28.3 bits (60), Expect = 6.4
Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
Frame = +2
Query: 227 CRPNP--APCSNSVAKQGDPLYLHPHTVLITKSGVIQLIMKSKLPYAIELQEWLL 385
C P+P A V + G+P PH L + ++ + + LP + QEWLL
Sbjct: 33 CPPSPLAATMKVKVKENGEPSAKPPHPDLKYLNQILHVPTRELLPEIDDDQEWLL 87
>At4g37060.1 68417.m05248 patatin, putative similar to patatin-like
latex allergen [Hevea brasiliensis][PMID:10589016];
contains patatin domain PF01734
Length = 414
Score = 27.9 bits (59), Expect = 8.5
Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 2/66 (3%)
Frame = +2
Query: 263 AKQGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLLEEVIPQV-LCTGKYAPAV 436
A Q + YL L + + L KS L I+L E +L + Q+ + TG Y PA
Sbjct: 313 ALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKMLTNRVMQMNIDTGTYEPAA 372
Query: 437 EMDTND 454
E ND
Sbjct: 373 ENINND 378
>At4g25320.1 68417.m03643 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 404
Score = 27.9 bits (59), Expect = 8.5
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +1
Query: 193 TSTASTNRRLSMPTKSSTMLQ*RGKAGRPAVFAP 294
T A+T L+MPT++++ Q + K GRP + P
Sbjct: 64 TENAATPFSLTMPTENTSAEQLKKKRGRPRKYNP 97
>At1g09970.2 68414.m01124 leucine-rich repeat transmembrane protein
kinase, putative Similar to A. thaliana receptor-like
protein kinase (gb|RLK5_ARATH). ESTs
gb|ATTS0475,gb|ATTS4362 come from this gene isoform
contains a TG acceptor site at intron.
Length = 977
Score = 27.9 bits (59), Expect = 8.5
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +3
Query: 666 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGPRLKRLGSNDVIFS 800
PS+ + C SL D+GNN F+ P+ SL +L+ L N+ FS
Sbjct: 114 PSDLKNCTSLKYLDLGNNLFSGAFPEFSSLN-QLQFLYLNNSAFS 157
>At1g09970.1 68414.m01123 leucine-rich repeat transmembrane protein
kinase, putative Similar to A. thaliana receptor-like
protein kinase (gb|RLK5_ARATH). ESTs
gb|ATTS0475,gb|ATTS4362 come from this gene isoform
contains a TG acceptor site at intron.
Length = 976
Score = 27.9 bits (59), Expect = 8.5
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +3
Query: 666 PSNPQLCHSLAVCDVGNNEFAFLRPQKRSLGPRLKRLGSNDVIFS 800
PS+ + C SL D+GNN F+ P+ SL +L+ L N+ FS
Sbjct: 114 PSDLKNCTSLKYLDLGNNLFSGAFPEFSSLN-QLQFLYLNNSAFS 157
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,120,784
Number of Sequences: 28952
Number of extensions: 397272
Number of successful extensions: 1079
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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