BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120168.Seq (679 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 138 9e-34 SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 132 3e-32 SPBC30B4.04c |sol1||SWI/SNF complex subunit Sol1|Schizosaccharom... 27 3.3 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 26 4.4 SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 7.6 SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 25 7.6 >SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase Tdh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 138 bits (333), Expect = 9e-34 Identities = 86/210 (40%), Positives = 110/210 (52%), Gaps = 4/210 (1%) Frame = +1 Query: 58 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 234 K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64 Query: 235 GFLVVNGTKLPFSQKGTLRPFHGEKLGLNML*SLLVSLPLQIKHLLTWREVLKKLLYQLP 414 G LV++G + + G + + Q + K+++ P Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124 Query: 415 VLMPPCLLWVLT*KLMTP---F*GHLKCFLHHKLFAPLAKVIQDNFEIFKGLMXXXXXXX 585 P + + + P + C + APLAKVI D F I +GLM Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTN--CLAPLAKVINDTFGIEEGLMTTVHATT 182 Query: 586 XXXXXXDGLSGKLWRDGRGAQQNIIPASTG 675 DG S K WR GRGA NIIP+STG Sbjct: 183 ATQKTVDGPSKKDWRGGRGASANIIPSSTG 212 Score = 104 bits (250), Expect = 1e-23 Identities = 48/71 (67%), Positives = 58/71 (81%) Frame = +3 Query: 255 NKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSADAPMF 434 N I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS DAPM+ Sbjct: 72 NAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSKDAPMY 131 Query: 435 VVGVNLEAYDP 467 VVGVN E ++P Sbjct: 132 VVGVNEEKFNP 142 Score = 34.3 bits (75), Expect = 0.016 Identities = 15/22 (68%), Positives = 17/22 (77%) Frame = +2 Query: 464 PPFKVISNASCTTNCLPHLQRL 529 P KVISNASCTTNCL L ++ Sbjct: 142 PSEKVISNASCTTNCLAPLAKV 163 >SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 132 bits (320), Expect = 3e-32 Identities = 84/210 (40%), Positives = 108/210 (51%), Gaps = 4/210 (1%) Frame = +1 Query: 58 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 234 K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64 Query: 235 GFLVVNGTKLPFSQKGTLRPFHGEKLGLNML*SLLVSLPLQIKHLLTWREVLKKLLYQLP 414 G LV++G + + G + + + K+++ P Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124 Query: 415 VLMPPCLLWVLT*KLMTP---F*GHLKCFLHHKLFAPLAKVIQDNFEIFKGLMXXXXXXX 585 P + + + P + C + APLAKVI D F I +GLM Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTN--CLAPLAKVINDTFGIEEGLMTTVHATT 182 Query: 586 XXXXXXDGLSGKLWRDGRGAQQNIIPASTG 675 DG S K WR GRGA NIIP+STG Sbjct: 183 ATQKTVDGPSKKDWRGGRGASANIIPSSTG 212 Score = 108 bits (259), Expect = 8e-25 Identities = 50/71 (70%), Positives = 59/71 (83%) Frame = +3 Query: 255 NKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSADAPMF 434 + I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS DAPMF Sbjct: 72 HSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSKDAPMF 131 Query: 435 VVGVNLEAYDP 467 VVGVNLE ++P Sbjct: 132 VVGVNLEKFNP 142 Score = 34.3 bits (75), Expect = 0.016 Identities = 15/22 (68%), Positives = 17/22 (77%) Frame = +2 Query: 464 PPFKVISNASCTTNCLPHLQRL 529 P KVISNASCTTNCL L ++ Sbjct: 142 PSEKVISNASCTTNCLAPLAKV 163 >SPBC30B4.04c |sol1||SWI/SNF complex subunit Sol1|Schizosaccharomyces pombe|chr 2|||Manual Length = 865 Score = 26.6 bits (56), Expect = 3.3 Identities = 23/67 (34%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = -3 Query: 377 QVSRCFICSGKDTSRLYNIFSPSFSPWNGLRVPF*ENGNFVPLTTRNPS*T-STLPLKRP 201 Q+S+ FI D +R +F SFSP+N + N L T PS T +T P P Sbjct: 613 QLSQNFILPNVDVARHVLLFILSFSPFNRKKSKTILNDT---LPTSIPSYTPATHPYAGP 669 Query: 200 WVESYLK 180 + +Y K Sbjct: 670 AINAYAK 676 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 26.2 bits (55), Expect = 4.4 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 3/54 (5%) Frame = -2 Query: 261 FCSVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 109 F + N+ESIL+L L + + E I++IV+ +D R HLS L + Sbjct: 339 FKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392 >SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 502 Score = 25.4 bits (53), Expect = 7.6 Identities = 14/37 (37%), Positives = 19/37 (51%) Frame = +1 Query: 73 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 183 GF RI +R EKG QVV D I + + +L+ Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334 >SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 609 Score = 25.4 bits (53), Expect = 7.6 Identities = 16/77 (20%), Positives = 38/77 (49%), Gaps = 6/77 (7%) Frame = +1 Query: 40 LADNMSKIGINGFGRIGRLVLRASIEKGAQVVAINDPFIGLD------YMVYLFKYDSTH 201 + D+ ++ +N F +G+L++ + ++ + ND ++ YM Y+F+ + Sbjct: 515 VGDHTGQLWLNVFDDVGKLIMHKTADE-LNDLQENDENAFMNCMAEACYMPYIFQCRAKQ 573 Query: 202 GRFKGSVEVQDGFLVVN 252 FKG + V+ + +N Sbjct: 574 DNFKGEMRVRYTVMSIN 590 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,041,224 Number of Sequences: 5004 Number of extensions: 65144 Number of successful extensions: 194 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 183 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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