BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120125.Seq
(707 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 113 2e-26
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 96 5e-21
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 93 3e-20
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 93 4e-20
SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 91 1e-19
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 80 4e-16
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 75 8e-15
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 73 5e-14
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 60 2e-10
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 30 0.37
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 29 0.86
SPAC5H10.01 |||DUF1445 family protein|Schizosaccharomyces pombe|... 27 2.6
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 27 2.6
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 25 8.0
SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr... 25 8.0
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 113 bits (272), Expect = 2e-26
Identities = 51/64 (79%), Positives = 59/64 (92%)
Frame = +3
Query: 513 LGQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLATDIL 692
LG+EVGY+IRFEDCSGP TLLKYMTDGMLLREAM+D M+ +Y I+LDEAHERTLATDIL
Sbjct: 150 LGEEVGYNIRFEDCSGPNTLLKYMTDGMLLREAMTDHMLSRYSCIILDEAHERTLATDIL 209
Query: 693 MGVL 704
MG++
Sbjct: 210 MGLM 213
Score = 89.4 bits (212), Expect = 4e-19
Identities = 45/85 (52%), Positives = 64/85 (75%)
Frame = +1
Query: 253 FHELLRKRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKA 432
+ ++L R LPV++ + +F+++ + +Q IV VGETGSGKTTQIPQ+ V + + L
Sbjct: 65 YFKILETRRELPVYQQREEFLKIYHENQIIVFVGETGSGKTTQIPQF-VLYDELPHLTNT 123
Query: 433 KGVACTQPRRVAAMSVAQRVAEEMD 507
+ +ACTQPRRVAAMSVA+RVA+EMD
Sbjct: 124 Q-IACTQPRRVAAMSVAKRVADEMD 147
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 95.9 bits (228), Expect = 5e-21
Identities = 44/71 (61%), Positives = 56/71 (78%)
Frame = +3
Query: 492 SRRNGWALGQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHER 671
S G LG VGYSIRFED +GP T++KYMTDG+LLRE++ +E+Y VI++DEAHER
Sbjct: 558 SEEMGVRLGSTVGYSIRFEDVTGPDTVIKYMTDGVLLRESLMQNNLEKYSVIIMDEAHER 617
Query: 672 TLATDILMGVL 704
+L TDILMG+L
Sbjct: 618 SLNTDILMGLL 628
Score = 70.9 bits (166), Expect = 2e-13
Identities = 35/74 (47%), Positives = 50/74 (67%)
Frame = +1
Query: 283 LPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKGVACTQPRR 462
LP + + + ++ +Q +++VGETGSGKTTQ+ Q+ E G + + CTQPRR
Sbjct: 492 LPAFAVREQLLSVIRDNQVLIVVGETGSGKTTQLAQFLYE----DGYHRNGMIGCTQPRR 547
Query: 463 VAAMSVAQRVAEEM 504
VAAMSVA+RV+EEM
Sbjct: 548 VAAMSVAKRVSEEM 561
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 93.5 bits (222), Expect = 3e-20
Identities = 41/67 (61%), Positives = 54/67 (80%)
Frame = +3
Query: 504 GWALGQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLAT 683
G +G+EVGY+IRFED + T +KYMTDGML RE + DP++ +Y VI+LDEAHERT+AT
Sbjct: 581 GCRVGEEVGYTIRFEDKTSRMTQIKYMTDGMLQRECLVDPLLSKYSVIILDEAHERTVAT 640
Query: 684 DILMGVL 704
D+L G+L
Sbjct: 641 DVLFGLL 647
Score = 82.6 bits (195), Expect = 5e-17
Identities = 42/78 (53%), Positives = 57/78 (73%)
Frame = +1
Query: 271 KRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKGVACT 450
+R GLPV++ + F+ ++ +Q +VL+GETGSGKTTQI Q+ E S +K + CT
Sbjct: 506 QREGLPVFKLRKQFLEAVSKNQILVLLGETGSGKTTQITQYLAEEGYTS---DSKMIGCT 562
Query: 451 QPRRVAAMSVAQRVAEEM 504
QPRRVAAMSVA+RVAEE+
Sbjct: 563 QPRRVAAMSVAKRVAEEV 580
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 93.1 bits (221), Expect = 4e-20
Identities = 39/65 (60%), Positives = 54/65 (83%)
Frame = +3
Query: 504 GWALGQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLAT 683
G LG++VGYSIRF+D + +T +KY+TDGMLLRE ++DP++ QY ++LDEAHERTL T
Sbjct: 166 GCRLGEQVGYSIRFDDTTSKKTRIKYLTDGMLLRELINDPILSQYHTLILDEAHERTLMT 225
Query: 684 DILMG 698
D+L+G
Sbjct: 226 DMLLG 230
Score = 69.3 bits (162), Expect = 5e-13
Identities = 37/81 (45%), Positives = 53/81 (65%)
Frame = +1
Query: 259 ELLRKRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKG 438
++L +R LP+WE + + + ++ IV+VGETGSGK+TQIPQ F +
Sbjct: 88 DILEQRKNLPIWEAHDTLCQQIQDNRVIVVVGETGSGKSTQIPQ----FLNECPYAQEGC 143
Query: 439 VACTQPRRVAAMSVAQRVAEE 501
VA TQPRRVAA+++A+RVA E
Sbjct: 144 VAITQPRRVAAVNLAKRVAAE 164
>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1055
Score = 91.5 bits (217), Expect = 1e-19
Identities = 40/64 (62%), Positives = 54/64 (84%)
Frame = +3
Query: 513 LGQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLATDIL 692
LGQEVGYSIRFE+ + +T++KY+TDGMLLRE +++P + Y VI++DEAHERTL TDIL
Sbjct: 492 LGQEVGYSIRFENATSEKTVIKYLTDGMLLREFLTEPDLASYSVIIIDEAHERTLHTDIL 551
Query: 693 MGVL 704
G++
Sbjct: 552 FGLV 555
Score = 89.4 bits (212), Expect = 4e-19
Identities = 43/78 (55%), Positives = 57/78 (73%)
Frame = +1
Query: 274 RLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKGVACTQ 453
R LPV++YK+D ++ +N +Q +++V ETGSGKTTQ+PQ+ E G K + CTQ
Sbjct: 415 RKSLPVYQYKDDLLKAINEYQVLLIVAETGSGKTTQLPQFLHEAGYTKG---NKKICCTQ 471
Query: 454 PRRVAAMSVAQRVAEEMD 507
PRRVAAMSVA RVA+EMD
Sbjct: 472 PRRVAAMSVAARVAKEMD 489
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 79.8 bits (188), Expect = 4e-16
Identities = 34/63 (53%), Positives = 48/63 (76%)
Frame = +3
Query: 516 GQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLATDILM 695
G GYSI+F+ +T +KYMTDG+LL E DP++E+Y +++LDE HERTL+TD+L+
Sbjct: 100 GSLCGYSIQFDHNVSEKTKIKYMTDGILLNEIFFDPLLERYSIVILDEVHERTLSTDLLL 159
Query: 696 GVL 704
GVL
Sbjct: 160 GVL 162
Score = 64.9 bits (151), Expect = 1e-11
Identities = 33/81 (40%), Positives = 53/81 (65%)
Frame = +1
Query: 265 LRKRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKGVA 444
++K+L LP+ +Y+N + + +Q +++G TG GKTTQIPQ+ E S G +
Sbjct: 20 IQKKL-LPITKYRNQLLYAVEQNQITIVLGHTGCGKTTQIPQFLYEAGWASQNGI---IG 75
Query: 445 CTQPRRVAAMSVAQRVAEEMD 507
CTQPRR+ A SV++RV+ E++
Sbjct: 76 CTQPRRLVAKSVSERVSLELN 96
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 75.4 bits (177), Expect = 8e-15
Identities = 36/81 (44%), Positives = 53/81 (65%)
Frame = +1
Query: 265 LRKRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKGVA 444
+R+R LP WE + M + Q +V+ GETGSGK+TQ+ Q+ ++ SG + V
Sbjct: 571 VREREKLPAWESRRKIMDAIQHSQVVVISGETGSGKSTQVVQFILDHYLSSGEKDLQTVV 630
Query: 445 CTQPRRVAAMSVAQRVAEEMD 507
CTQPRR++A+S+A+RVA E D
Sbjct: 631 CTQPRRISAISLAERVAFERD 651
Score = 50.0 bits (114), Expect = 3e-07
Identities = 24/66 (36%), Positives = 40/66 (60%), Gaps = 2/66 (3%)
Frame = +3
Query: 513 LGQEVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPM--VEQYRVILLDEAHERTLATD 686
+G+EVGYS+ E +TLL++ T G+LLR + + +++DE HER++ D
Sbjct: 654 VGKEVGYSVHGEKSISKETLLEFCTTGLLLRRIQQHGLGFLSTLSCVVVDEVHERSIEND 713
Query: 687 ILMGVL 704
IL+ +L
Sbjct: 714 ILLTLL 719
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1183
Score = 72.5 bits (170), Expect = 5e-14
Identities = 31/61 (50%), Positives = 45/61 (73%)
Frame = +3
Query: 522 EVGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLATDILMGV 701
+V Y IRF+ P T +K+MTDG+LLRE SD ++ Y +++DEAHER++ TDIL+G+
Sbjct: 478 KVSYQIRFDSTINPDTAIKFMTDGILLRELSSDFLLTAYSAVIVDEAHERSVNTDILLGL 537
Query: 702 L 704
L
Sbjct: 538 L 538
Score = 70.1 bits (164), Expect = 3e-13
Identities = 36/85 (42%), Positives = 53/85 (62%), Gaps = 1/85 (1%)
Frame = +1
Query: 259 ELLRKRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKG 438
E+ RL LP+ + M + + +++ G TGSGKTTQ+PQ+ E S + G
Sbjct: 390 EIQESRLALPIVAEEQRIMEQIFANDVVIICGATGSGKTTQLPQFLFEAGFSSPESENPG 449
Query: 439 -VACTQPRRVAAMSVAQRVAEEMDG 510
+A TQPRRVAA+S+A+RV+EE+ G
Sbjct: 450 MIAITQPRRVAAVSIAKRVSEELTG 474
>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1428
Score = 60.5 bits (140), Expect = 2e-10
Identities = 28/76 (36%), Positives = 50/76 (65%)
Frame = +1
Query: 283 LPVWEYKNDFMRLLNTHQCIVLVGETGSGKTTQIPQWSVEFAAVSGLGKAKGVACTQPRR 462
LP ++ + +N Q +++ G+TG GK+TQIP + +E + + GKA + T+PRR
Sbjct: 631 LPATMFRETIIDAVNNSQLLIISGDTGCGKSTQIPAFLLENSTKN--GKAVKIYVTEPRR 688
Query: 463 VAAMSVAQRVAEEMDG 510
++A+S+A RV++E+ G
Sbjct: 689 ISAISLANRVSQELGG 704
Score = 45.2 bits (102), Expect = 9e-06
Identities = 21/57 (36%), Positives = 33/57 (57%)
Frame = +3
Query: 525 VGYSIRFEDCSGPQTLLKYMTDGMLLREAMSDPMVEQYRVILLDEAHERTLATDILM 695
VGYS+R + P T L Y+T G LR +E +++DE HER++ +D+L+
Sbjct: 715 VGYSVRLDSKCTPLTPLTYVTTGTFLRLLEVGNEIESVTHLIIDEVHERSIDSDLLL 771
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 29.9 bits (64), Expect = 0.37
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = +1
Query: 322 LNTHQCIVLVGETGSGKTTQI 384
+ T++ ++LVGETG+GKTT I
Sbjct: 464 VQTNEPLLLVGETGTGKTTTI 484
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 316 RLLNTHQCIVLVGETGSGKTT 378
R L + ++LVG+TG GKTT
Sbjct: 1181 RCLLAKEPVLLVGDTGCGKTT 1201
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 28.7 bits (61), Expect = 0.86
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +1
Query: 313 MRLLNTHQCIVLVGETGSGKT 375
M+ + +QCI++ GE+G+GKT
Sbjct: 120 MKSYHENQCIIISGESGAGKT 140
>SPAC5H10.01 |||DUF1445 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 301
Score = 27.1 bits (57), Expect = 2.6
Identities = 12/39 (30%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +1
Query: 226 IYWSSAFTTFHELLRKRLGLPVWEYKNDFMRLLN-THQC 339
I+W T + ++ +L PV+ +K +M L + TH C
Sbjct: 263 IFWGCGVTPQNVVMMSKLPEPVYSHKPGYMYLTDLTHNC 301
>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1516
Score = 27.1 bits (57), Expect = 2.6
Identities = 18/69 (26%), Positives = 30/69 (43%), Gaps = 3/69 (4%)
Frame = +1
Query: 226 IYWSSAFTTFHELLRKRLGLPVWEYKNDFMRLLNT---HQCIVLVGETGSGKTTQIPQWS 396
+Y + E R L ++ D + +N +Q I++ GE+G+GKT
Sbjct: 122 LYTHEIVRAYSEKSRDELDPHLYAIAEDSYKCMNQEHKNQTIIISGESGAGKTVSARYIM 181
Query: 397 VEFAAVSGL 423
FA+V L
Sbjct: 182 RYFASVQAL 190
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 25.4 bits (53), Expect = 8.0
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 256 HELLRKRLGLPVWEYKNDFMRLLNTHQCIVLVGETGSGKTT 378
H +R R LP+ ND + + I +VG TG+GK+T
Sbjct: 1228 HYSVRYRENLPL--VLNDISVNIKPQEKIGIVGRTGAGKST 1266
>SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 593
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +1
Query: 334 QCIVLVGETGSGKTT 378
+ IVL+GE G+GKTT
Sbjct: 376 EIIVLLGENGTGKTT 390
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,958,670
Number of Sequences: 5004
Number of extensions: 62249
Number of successful extensions: 197
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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