BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120119.Seq (649 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26A3.07c |rpl1101|rpl11-1, rpl11|60S ribosomal protein L11|S... 157 2e-39 SPBC17G9.10 |rpl1102|rpl11-2|60S ribosomal protein L11|Schizosac... 157 2e-39 SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |... 28 1.0 SPCC1393.13 |||DUF89 family protein|Schizosaccharomyces pombe|ch... 28 1.0 SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 27 1.8 SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Ma... 27 2.3 SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 26 5.4 SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 25 9.4 SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 25 9.4 >SPAC26A3.07c |rpl1101|rpl11-1, rpl11|60S ribosomal protein L11|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 157 bits (380), Expect = 2e-39 Identities = 72/85 (84%), Positives = 77/85 (90%) Frame = +2 Query: 254 FGIRRNEKIAVHCTVRGAKAEEILERGLKVREYELRRDNFSATGNFGFGIQEHIDLGIKY 433 FGIRRNEKIA H TVRG KAEEILERGLKV+EYEL++ NFSATGNFGFGIQEHIDLGIKY Sbjct: 57 FGIRRNEKIACHVTVRGPKAEEILERGLKVKEYELKKRNFSATGNFGFGIQEHIDLGIKY 116 Query: 434 DPSIGIYGLDFYVVLGRPGFNVAHR 508 DPSIGIYG+DFYVV+ RPG VA R Sbjct: 117 DPSIGIYGMDFYVVMDRPGMRVARR 141 Score = 81.0 bits (191), Expect = 1e-16 Identities = 38/53 (71%), Positives = 45/53 (84%) Frame = +3 Query: 93 DNSKNVMRNLHIRKLCLNICVGESGDRLTRAAKVLEQLTGQQPVFSKARYTVR 251 + ++N M+ L I KL LNI +GESGDRLTRAAKVLEQL+GQ PVFSKARYT+R Sbjct: 3 EKAQNPMKELRISKLVLNISLGESGDRLTRAAKVLEQLSGQTPVFSKARYTIR 55 Score = 42.7 bits (96), Expect = 4e-05 Identities = 16/33 (48%), Positives = 24/33 (72%), Gaps = 2/33 (6%) Frame = +1 Query: 508 RRKT--GKVGFPHRLTKEDAMKWFQQKYDGIIL 600 RRK G+VG+ H++ ED + WF+QKYD ++L Sbjct: 140 RRKAQRGRVGYTHKINAEDTINWFKQKYDAVVL 172 >SPBC17G9.10 |rpl1102|rpl11-2|60S ribosomal protein L11|Schizosaccharomyces pombe|chr 2|||Manual Length = 174 Score = 157 bits (380), Expect = 2e-39 Identities = 72/85 (84%), Positives = 77/85 (90%) Frame = +2 Query: 254 FGIRRNEKIAVHCTVRGAKAEEILERGLKVREYELRRDNFSATGNFGFGIQEHIDLGIKY 433 FGIRRNEKIA H TVRG KAEEILERGLKV+EYEL++ NFSATGNFGFGIQEHIDLGIKY Sbjct: 57 FGIRRNEKIACHVTVRGPKAEEILERGLKVKEYELKKRNFSATGNFGFGIQEHIDLGIKY 116 Query: 434 DPSIGIYGLDFYVVLGRPGFNVAHR 508 DPSIGIYG+DFYVV+ RPG VA R Sbjct: 117 DPSIGIYGMDFYVVMDRPGMRVARR 141 Score = 81.0 bits (191), Expect = 1e-16 Identities = 38/53 (71%), Positives = 45/53 (84%) Frame = +3 Query: 93 DNSKNVMRNLHIRKLCLNICVGESGDRLTRAAKVLEQLTGQQPVFSKARYTVR 251 + ++N M+ L I KL LNI +GESGDRLTRAAKVLEQL+GQ PVFSKARYT+R Sbjct: 3 EKAQNPMKELRISKLVLNISLGESGDRLTRAAKVLEQLSGQTPVFSKARYTIR 55 Score = 42.7 bits (96), Expect = 4e-05 Identities = 16/33 (48%), Positives = 24/33 (72%), Gaps = 2/33 (6%) Frame = +1 Query: 508 RRKT--GKVGFPHRLTKEDAMKWFQQKYDGIIL 600 RRK G+VG+ H++ ED + WF+QKYD ++L Sbjct: 140 RRKAQRGRVGYTHKINAEDTINWFKQKYDAVVL 172 >SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 791 Score = 28.3 bits (60), Expect = 1.0 Identities = 16/53 (30%), Positives = 28/53 (52%) Frame = +1 Query: 463 LLRRTWPARFQRSTQRRKTGKVGFPHRLTKEDAMKWFQQKYDGIILNSKAK*N 621 ++ +T PA+ + QRRK GK + K+ ++K + + + N KAK N Sbjct: 472 VINQTGPAK-KTPVQRRKDGKFAKSTKRKKQKSLKPDTENQESSVKNKKAKSN 523 >SPCC1393.13 |||DUF89 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 442 Score = 28.3 bits (60), Expect = 1.0 Identities = 14/52 (26%), Positives = 28/52 (53%) Frame = -1 Query: 517 SYASVCYVETWPAKYDVKVQSINSN*GIVLDTQVNVFLNTEAKITRGGEVVT 362 SYASVC ++ WP + ++ N L+ ++ + A +T+G E+++ Sbjct: 20 SYASVCVMKKWPIIATNVIDEVSRNISKALEAGMS---DKAAYVTQGKEIIS 68 >SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1115 Score = 27.5 bits (58), Expect = 1.8 Identities = 11/24 (45%), Positives = 18/24 (75%) Frame = -2 Query: 126 YEDSALHF*NYPSEPSSPFYLSST 55 YE+ + + +Y S+PSSP Y+SS+ Sbjct: 153 YEEDSYNNYDYTSDPSSPNYISSS 176 >SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 387 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -2 Query: 246 LYT*PWKIQAVVL*VAPTPWRHESACHRIHQHI 148 +Y W I A +L V P PWR+ S+ + + I Sbjct: 127 IYPLLWVITAFILIVFPFPWRYRSSQRGLRKSI 159 >SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor Sec74|Schizosaccharomyces pombe|chr 1|||Manual Length = 928 Score = 25.8 bits (54), Expect = 5.4 Identities = 18/60 (30%), Positives = 26/60 (43%) Frame = +3 Query: 150 CVGESGDRLTRAAKVLEQLTGQQPVFSKARYTVRSLVSVVMKRLLSIVQSEELKQKKSLR 329 CV S + + VL + SKA RS V K + IVQ+ K +++LR Sbjct: 691 CVESSDKSIKASVSVLPDIYEDSDAMSKASKETRSEVLECSKVRVYIVQNRIYKLEEALR 750 >SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 1405 Score = 25.0 bits (52), Expect = 9.4 Identities = 10/23 (43%), Positives = 13/23 (56%) Frame = +1 Query: 442 NWNLWTGLLRRTWPARFQRSTQR 510 NW+ L R WP F RS+Q+ Sbjct: 644 NWSSIGRLRRSEWPRSFNRSSQQ 666 >SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1053 Score = 25.0 bits (52), Expect = 9.4 Identities = 6/13 (46%), Positives = 12/13 (92%) Frame = -3 Query: 575 WNHFIASSFVRRW 537 W+H+I++SF+ +W Sbjct: 519 WSHYISASFLSKW 531 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,635,368 Number of Sequences: 5004 Number of extensions: 55741 Number of successful extensions: 164 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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