Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120110.Seq
(826 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 135 3e-32
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 132 3e-31
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 131 5e-31
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 39 0.005
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 39 0.005
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 35 0.075
At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki... 30 1.6
At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17 si... 29 2.8
At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17 si... 29 2.8
At2g02610.1 68415.m00200 DC1 domain-containing protein contain... 29 5.0
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 6.5
At3g27670.1 68416.m03455 expressed protein 28 8.7
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 135 bits (327), Expect = 3e-32
Identities = 59/87 (67%), Positives = 71/87 (81%)
Frame = +3
Query: 501 GYHVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 680
G +CN+E +GDRG LARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M+G V
Sbjct: 111 GAVICNVELHVGDRGALARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQV 170
Query: 681 AGGGRIDKPILKAGRAYHKYKVKRNCW 761
AGGGR +KP LKAG AYHKYK KRNCW
Sbjct: 171 AGGGRTEKPFLKAGNAYHKYKAKRNCW 197
Score = 84.2 bits (199), Expect = 9e-17
Identities = 36/54 (66%), Positives = 45/54 (83%)
Frame = +2
Query: 257 KLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 418
K RSLDY ER+GY+KG+V +IIHDPGRGAPLA V FR P+++ +KELF+A EG
Sbjct: 29 KFRSLDYGERNGYLKGLVTEIIHDPGRGAPLARVAFRHPFRYMKQKELFVAAEG 82
Score = 54.0 bits (124), Expect = 1e-07
Identities = 20/31 (64%), Positives = 28/31 (90%)
Frame = +1
Query: 418 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTM 510
+YTGQ++YCGKKA L VGNV+P+G++PEG +
Sbjct: 83 MYTGQYLYCGKKANLMVGNVLPLGSIPEGAV 113
Score = 51.6 bits (118), Expect = 6e-07
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2
Query: 767 VRGVAMNPVEHPHGGGNHQH 826
VRGVAMNPVEHPHGGGNHQH
Sbjct: 200 VRGVAMNPVEHPHGGGNHQH 219
Score = 44.4 bits (100), Expect = 9e-05
Identities = 23/30 (76%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = +3
Query: 177 MGRVIRAQRKG-AGSVFVSHTKKRKGALNF 263
MGRVIRAQRKG AGSVF SHT RKG F
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKF 30
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 132 bits (319), Expect = 3e-31
Identities = 58/87 (66%), Positives = 71/87 (81%)
Frame = +3
Query: 501 GYHVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 680
G VCN+E +GDRG LARASG++A VI HNPD+ TR+KLPSG+KK++PS R M+G V
Sbjct: 110 GAVVCNVEHHVGDRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQV 169
Query: 681 AGGGRIDKPILKAGRAYHKYKVKRNCW 761
AGGGR +KP+LKAG AYHKY+VKRN W
Sbjct: 170 AGGGRTEKPMLKAGNAYHKYRVKRNSW 196
Score = 87.4 bits (207), Expect = 1e-17
Identities = 38/54 (70%), Positives = 46/54 (85%)
Frame = +2
Query: 257 KLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 418
K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A EG
Sbjct: 28 KFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVTFRHPFRFKKQKELFVAAEG 81
Score = 54.0 bits (124), Expect = 1e-07
Identities = 21/31 (67%), Positives = 28/31 (90%)
Frame = +1
Query: 418 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTM 510
+YTGQF+YCGKKATL VGNV+P+ ++PEG +
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSIPEGAV 112
Score = 51.6 bits (118), Expect = 6e-07
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2
Query: 767 VRGVAMNPVEHPHGGGNHQH 826
VRGVAMNPVEHPHGGGNHQH
Sbjct: 199 VRGVAMNPVEHPHGGGNHQH 218
Score = 48.8 bits (111), Expect = 4e-06
Identities = 23/29 (79%), Positives = 23/29 (79%)
Frame = +3
Query: 177 MGRVIRAQRKGAGSVFVSHTKKRKGALNF 263
MGRVIRAQRKGAGSVF SHT RKG F
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKF 29
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 131 bits (317), Expect = 5e-31
Identities = 56/87 (64%), Positives = 70/87 (80%)
Frame = +3
Query: 501 GYHVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 680
G +CN+E +GDRG ARASG++A VI HNPD +R+KLPSG+KK++PS R M+G V
Sbjct: 110 GAVICNVEHHVGDRGVFARASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQV 169
Query: 681 AGGGRIDKPILKAGRAYHKYKVKRNCW 761
AGGGR +KP+LKAG AYHKY+VKRNCW
Sbjct: 170 AGGGRTEKPMLKAGNAYHKYRVKRNCW 196
Score = 87.4 bits (207), Expect = 1e-17
Identities = 38/54 (70%), Positives = 46/54 (85%)
Frame = +2
Query: 257 KLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 418
K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A EG
Sbjct: 28 KFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVAFRHPFRFKKQKELFVAAEG 81
Score = 54.0 bits (124), Expect = 1e-07
Identities = 21/31 (67%), Positives = 28/31 (90%)
Frame = +1
Query: 418 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTM 510
+YTGQF+YCGKKATL VGNV+P+ ++PEG +
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSIPEGAV 112
Score = 51.6 bits (118), Expect = 6e-07
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2
Query: 767 VRGVAMNPVEHPHGGGNHQH 826
VRGVAMNPVEHPHGGGNHQH
Sbjct: 199 VRGVAMNPVEHPHGGGNHQH 218
Score = 48.8 bits (111), Expect = 4e-06
Identities = 23/29 (79%), Positives = 23/29 (79%)
Frame = +3
Query: 177 MGRVIRAQRKGAGSVFVSHTKKRKGALNF 263
MGRVIRAQRKGAGSVF SHT RKG F
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKF 29
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 38.7 bits (86), Expect = 0.005
Identities = 22/76 (28%), Positives = 38/76 (50%)
Frame = +3
Query: 501 GYHVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 680
G + N+E G ++ RA+G A ++ P + +KLPSG K + + R +G V
Sbjct: 71 GTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTV 129
Query: 681 AGGGRIDKPILKAGRA 728
+ K + KAG++
Sbjct: 130 SNPSHGTKKLYKAGQS 145
Score = 37.5 bits (83), Expect = 0.011
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +2
Query: 767 VRGVAMNPVEHPHGGG 814
VRGVAMNP +HPHGGG
Sbjct: 154 VRGVAMNPCDHPHGGG 169
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 38.7 bits (86), Expect = 0.005
Identities = 22/76 (28%), Positives = 38/76 (50%)
Frame = +3
Query: 501 GYHVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 680
G + N+E G ++ RA+G A ++ P + +KLPSG K + + R +G V
Sbjct: 71 GTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTV 129
Query: 681 AGGGRIDKPILKAGRA 728
+ K + KAG++
Sbjct: 130 SNPSHGTKKLYKAGQS 145
Score = 37.5 bits (83), Expect = 0.011
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +2
Query: 767 VRGVAMNPVEHPHGGG 814
VRGVAMNP +HPHGGG
Sbjct: 154 VRGVAMNPCDHPHGGG 169
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 34.7 bits (76), Expect = 0.075
Identities = 16/18 (88%), Positives = 16/18 (88%), Gaps = 1/18 (5%)
Frame = +2
Query: 761 AYVRGVAM-NPVEHPHGG 811
A VRGVAM NPVEHPHGG
Sbjct: 420 AKVRGVAMMNPVEHPHGG 437
>At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate
5-kinase, putative / PIP kinase, putative /
PtdIns(4)P-5-kinase, putative / diphosphoinositide
kinase, putative similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 705
Score = 30.3 bits (65), Expect = 1.6
Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = -2
Query: 477 HISNFKSCFLSTINKLACVEPSGAMKSSFLVL-NLYGS 367
H+S +K+ ++ + CV+P G K+ F+V+ NL+ S
Sbjct: 462 HVSKYKNSLVTKFFGVHCVKPVGGQKTRFIVMGNLFCS 499
>At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 123
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -2
Query: 429 ACVEPSGAMKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 301
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 70 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 112
>At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 122
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -2
Query: 429 ACVEPSGAMKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 301
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 69 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 111
>At2g02610.1 68415.m00200 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 28.7 bits (61), Expect = 5.0
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = -2
Query: 555 VPDDLYHPFSLQDCTHGTLRHSSNRHHIS-NFKSCFLSTINKLACVEP 415
VP++L S + +G + H S+RHH+ + F+ ACV P
Sbjct: 327 VPEELEDVESFRKIANGVILHFSHRHHLKFDISGVFVGNKFCQACVLP 374
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 6.5
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +2
Query: 683 WRWTY*QTYFESWKGIPQVQG 745
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At3g27670.1 68416.m03455 expressed protein
Length = 1841
Score = 27.9 bits (59), Expect = 8.7
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = -2
Query: 480 HHISNFKSCFLSTINKLACVEPSGAMKSSFLVLNLYGS 367
H + + CF I++L V SGA++ L+ + G+
Sbjct: 1204 HEVDDIIGCFKDLISELLIVRKSGALRKRLLMASCIGA 1241
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,645,475
Number of Sequences: 28952
Number of extensions: 397363
Number of successful extensions: 1038
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 997
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1037
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1892353600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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