BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120101.Seq (771 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1399.03 |fur4||uracil permease|Schizosaccharomyces pombe|chr... 29 0.97 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 28 1.7 SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 27 2.2 SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 2.2 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 27 3.9 SPAC890.04c |||ribosome biogenesis protein Ytm1 |Schizosaccharom... 27 3.9 SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 26 5.2 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 26 6.9 SPBC21H7.04 |||ATP-dependent RNA helicase Dbp7 |Schizosaccharomy... 26 6.9 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 9.1 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 9.1 >SPAC1399.03 |fur4||uracil permease|Schizosaccharomyces pombe|chr 1|||Manual Length = 581 Score = 28.7 bits (61), Expect = 0.97 Identities = 15/47 (31%), Positives = 20/47 (42%) Frame = -3 Query: 322 LNHARFGNEHCVWVQIQRVALLCTLSGAWCWIWSACSNVDLYLPSTW 182 L+ + FG +W + R L C G WI C V L + S W Sbjct: 123 LSRSSFGTWGSLWPILNRSVLACVWYGVQAWIGGEC--VVLMIRSIW 167 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 27.9 bits (59), Expect = 1.7 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = -1 Query: 570 VSASRRATIISLANKMSDRVAPPNLRWPPSIFASDRQF 457 + S+ + +S N++S L++PPS F S RQF Sbjct: 198 LQTSQDSPSLSATNQLSSSDTLEPLQYPPSSFGSQRQF 235 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 27.5 bits (58), Expect = 2.2 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = +3 Query: 444 DVIAKIDDLTQKLTVANAD 500 D +KID LT+KL VANA+ Sbjct: 618 DSASKIDSLTEKLKVANAE 636 >SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 728 Score = 27.5 bits (58), Expect = 2.2 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +2 Query: 296 LITKSGVIQLIMKSKLPYAIELQEWLL 376 L+ K GV+ L+ K KLP+++ ++ ++ Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 26.6 bits (56), Expect = 3.9 Identities = 12/47 (25%), Positives = 26/47 (55%) Frame = +1 Query: 571 GSARLRKRAPRNGAVANRMADIAQDVIAKPSNPQLCHSLAVCDVGNN 711 G +++ KRA + + +AD+++ ++ S HS A+ ++G N Sbjct: 447 GGSQIEKRANESNNLQREIADLSEQIVELESKRNDLHS-ALLEMGGN 492 >SPAC890.04c |||ribosome biogenesis protein Ytm1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 440 Score = 26.6 bits (56), Expect = 3.9 Identities = 23/101 (22%), Positives = 51/101 (50%), Gaps = 6/101 (5%) Frame = -2 Query: 719 ANSLLPTSHT--ARLWHSCGLLGLAITSCAMSAMRLAT--APFRGARFRNLAEPFQRRV- 555 ++++L +S+ AR+W G + T C S+++ A+ P + +L + V Sbjct: 114 SDTILTSSYDGIARVWDKSGEIKFQSTGCG-SSLKSASWHIPNQSFLTASLDQKIFHWVI 172 Query: 554 -EPQSFRWQTK*AIGLLRQICVGHRQFLRQIVNFGNNIICI 435 EP+S K + G+L+ + VGH+ + ++ + ++ + I Sbjct: 173 EEPESMLDAEKKSSGILQTLFVGHKDIVERVRSLESSSVFI 213 >SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2176 Score = 26.2 bits (55), Expect = 5.2 Identities = 20/54 (37%), Positives = 24/54 (44%), Gaps = 2/54 (3%) Frame = -2 Query: 686 RLWHSCGLLGLAITSCAMSAMRL--ATAPFRGARFRNLAEPFQRRVEPQSFRWQ 531 R W S L L +C M RL +P R +F N RRVE + F WQ Sbjct: 1140 RGWSSVATLSL--DTCKMIEKRLWPTMSPLR--QFPNCPSEVIRRVEKKEFPWQ 1189 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.9 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -3 Query: 574 SRFSVASSHNHFVGKQNERSGCSAKSAL 491 S FS + SHNH Q E+S ++K ++ Sbjct: 537 SPFSKSKSHNHHPSSQVEKSTSNSKGSM 564 >SPBC21H7.04 |||ATP-dependent RNA helicase Dbp7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 709 Score = 25.8 bits (54), Expect = 6.9 Identities = 12/42 (28%), Positives = 22/42 (52%) Frame = +1 Query: 508 SNPIAHFVCQRNDCGSTRR*NGSARLRKRAPRNGAVANRMAD 633 +NP++H++ N G ++ + AR+RK R+AD Sbjct: 241 NNPLSHWIVSCNVIGGEKKKSEKARIRKGVNILIGTPGRLAD 282 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 9.1 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 99 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 224 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 9.1 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +2 Query: 527 LFANEMIVARRDAETARQDCE 589 +FANE+++ + DA++ +QD E Sbjct: 556 IFANEILIEQADAQSFKQDEE 576 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,174,128 Number of Sequences: 5004 Number of extensions: 66548 Number of successful extensions: 189 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 186 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 371330890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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