BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120092.Seq (736 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 113 3e-26 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 110 2e-25 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 42 7e-05 SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 34 0.018 SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 27 2.8 SPAC664.14 |amt2||ammonium transporter Amt2|Schizosaccharomyces ... 27 3.7 SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 26 6.4 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 6.4 SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 26 6.4 SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schiz... 25 8.5 SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosacc... 25 8.5 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 113 bits (271), Expect = 3e-26 Identities = 44/68 (64%), Positives = 55/68 (80%) Frame = -1 Query: 205 KPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYHFMGC 26 +PGDHV+ LY P+C CKFC + KTNLC K+R TQG+G+MPDGT RF C+ + L H+MGC Sbjct: 86 RPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDGTSRFSCRDKTLLHYMGC 145 Query: 25 STFSQYTV 2 S+FSQYTV Sbjct: 146 SSFSQYTV 153 Score = 103 bits (248), Expect = 2e-23 Identities = 49/72 (68%), Positives = 56/72 (77%) Frame = -2 Query: 459 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPE 280 MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ + VCHTDAYTLSG DPE Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLSGVDPE 60 Query: 279 GVFPVVLGLKAA 244 G FP+VLG + A Sbjct: 61 GAFPIVLGHEGA 72 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 110 bits (264), Expect = 2e-25 Identities = 44/66 (66%), Positives = 53/66 (80%) Frame = -1 Query: 199 GDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYHFMGCST 20 GD V+ LY P+C TCKFC + KTNLC ++R+TQG+G+MPDGT RF C G L HFMGCST Sbjct: 91 GDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGKGLMPDGTSRFSCNGNTLLHFMGCST 150 Query: 19 FSQYTV 2 FS+YTV Sbjct: 151 FSEYTV 156 Score = 107 bits (256), Expect = 2e-24 Identities = 46/70 (65%), Positives = 56/70 (80%) Frame = -2 Query: 453 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGV 274 T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI +GVCHTDAYTLSGKDPEG+ Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGL 65 Query: 273 FPVVLGLKAA 244 FPV+LG + A Sbjct: 66 FPVILGHEGA 75 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 42.3 bits (95), Expect = 7e-05 Identities = 28/67 (41%), Positives = 35/67 (52%), Gaps = 3/67 (4%) Frame = -2 Query: 435 KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDP-EGVFPV 265 K LAAV G P ++ EE+ V P EV V I TGVCHTD + L G P P+ Sbjct: 6 KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKMPL 65 Query: 264 VLGLKAA 244 + G + A Sbjct: 66 IGGHEGA 72 >SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 34.3 bits (75), Expect = 0.018 Identities = 15/47 (31%), Positives = 26/47 (55%) Frame = -2 Query: 396 LSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVLG 256 + I+ + + PK GE+ VKI A + +D +G P V+P ++G Sbjct: 23 IEIQSVPIPQPKNGELLVKIEAAAINPSDLMNATGGFPYTVYPRIVG 69 >SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr 2|||Manual Length = 1250 Score = 27.1 bits (57), Expect = 2.8 Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Frame = -1 Query: 199 GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 44 G HVV L V N C + KT KV + + P G+R++ C G L Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087 >SPAC664.14 |amt2||ammonium transporter Amt2|Schizosaccharomyces pombe|chr 1|||Manual Length = 512 Score = 26.6 bits (56), Expect = 3.7 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Frame = +3 Query: 645 WSSVGSCDDLLMNLVGNQP-SGFPLPSAS 728 WS++G C ++ LV P +GF P AS Sbjct: 298 WSTIGFCSGVVAGLVAATPCAGFVSPHAS 326 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 25.8 bits (54), Expect = 6.4 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = -1 Query: 205 KPGDHVVPLYVPQCNTCKFCLNPKTNLC 122 KPGD V C C +C + + NLC Sbjct: 84 KPGDPVAVEPGCVCRLCDYCRSGRYNLC 111 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 25.8 bits (54), Expect = 6.4 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = -1 Query: 712 GKPLG*FPTRFMSRSSQDPTDDHDHSVKS 626 G P+ F+S +S+ PTDD H+V S Sbjct: 210 GSPVRSSKNPFLSSNSRLPTDDSSHTVGS 238 >SPBC36.10 |||mitochondrial intermembrane space protein sorting protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 25.8 bits (54), Expect = 6.4 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +2 Query: 404 PASHATAAKHLITLPTVDMTALDNCQSQNVEIIVEYEALTR 526 P HAT H+I + T+D LDN +I ++AL R Sbjct: 26 PNEHAT---HVIAVDTLDRKVLDNGVLYTERLITCHQALPR 63 >SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schizosaccharomyces pombe|chr 1|||Manual Length = 666 Score = 25.4 bits (53), Expect = 8.5 Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%) Frame = -2 Query: 435 KCLAAVAWEAGKPL-SIEEIEVDPPKAGEVRVKITATG 325 K ++ +AWE KPL + E+I D +GE+ V T +G Sbjct: 63 KVISCIAWEQ-KPLYASEQITTDISGSGEILVLGTNSG 99 >SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 25.4 bits (53), Expect = 8.5 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 2/51 (3%) Frame = -2 Query: 594 LDRKPFLMPTKFVLSDSDILLHHRVSASYSTIISTFCDWQL--SSAVMSTV 448 +D+K +L+P+ + ++ R+ S I F D L ++A+MST+ Sbjct: 44 IDKKKYLVPSDLTVGQFVYVIRKRIKLSPEKAIFIFIDEILPPTAALMSTI 94 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,097,431 Number of Sequences: 5004 Number of extensions: 61827 Number of successful extensions: 169 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 169 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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