BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120082.Seq (746 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 30 0.30 SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory su... 28 1.2 SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 27 2.1 SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr 2|... 27 3.8 SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 26 6.6 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 8.7 SPAC139.02c |oac1||anion transporter|Schizosaccharomyces pombe|c... 25 8.7 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 30.3 bits (65), Expect = 0.30 Identities = 17/57 (29%), Positives = 33/57 (57%), Gaps = 3/57 (5%) Frame = +2 Query: 251 DYSKHKKFVYWPKDTNALVPLVWRESKEIKLPYKTLSHNLS-KIIKV--YVYQHDKI 412 DY K + ++ N +W+ E K+PY +LS++LS +I+K+ +Y ++K+ Sbjct: 975 DYVNEVKRII-QRNANLEFEAIWKGHSENKIPYTSLSNHLSTEIVKLDHDIYNYEKL 1030 >SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory subunit Rpn12|Schizosaccharomyces pombe|chr 2|||Manual Length = 270 Score = 28.3 bits (60), Expect = 1.2 Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 3/64 (4%) Frame = +2 Query: 236 IKREKDYSKHKKFVYWPKDTNALVP---LVWRESKEIKLPYKTLSHNLSKIIKVYVYQHD 406 + E+ + +Y+PK+ NAL ++ E E++LP H +S I ++ Y + Sbjct: 206 LAEERGWDIRDGVIYFPKEANALETEDGMLIDEEDELELPPTASKHTISSIRQLLSYTSE 265 Query: 407 KIEI 418 +I Sbjct: 266 LEQI 269 >SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1040 Score = 27.5 bits (58), Expect = 2.1 Identities = 11/40 (27%), Positives = 23/40 (57%) Frame = +3 Query: 54 GNFMIEKEISYSINFSQDLLYKILNSYIVPNYSLAQQYFD 173 G+F+ + +++ I S+D+ + N I P S+ ++Y D Sbjct: 763 GHFLCRECLTHVITSSEDMAKQTSNENISPKCSVCEEYID 802 >SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 26.6 bits (56), Expect = 3.8 Identities = 27/131 (20%), Positives = 54/131 (41%), Gaps = 3/131 (2%) Frame = +2 Query: 164 IFRFVRRKRLSHSYTYSERLQ*HNIKREK---DYSKHKKFVYWPKDTNALVPLVWRESKE 334 ++ ++ K + T+S+ L H I K D K F + + P+ SK Sbjct: 36 LYHALKAKESTCFLTFSKTLDEHAISMRKWGMDIKTKKNFFFIDGFSMLFAPI----SKP 91 Query: 335 IKLPYKTLSHNLSKIIKVYVYQHDKIEIKFEHVYFSKSDIDLFDSTMANKISKLLTLLKM 514 K+ +++ + + ++ + +FE+ DID+ ST A +K ++ Sbjct: 92 SKVQAPETKNHIKSVFAPVIQCVEENDFEFENSTIIIEDIDILQSTHALDSTK----IQQ 147 Query: 515 ETLHRRRCKTR 547 L R+C +R Sbjct: 148 AILELRKCFSR 158 >SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1639 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = -2 Query: 727 GAILSINVVSGTNGDMFCASVNASMSAIMWHITFS 623 G ++S +V NG M C S + MW TF+ Sbjct: 1188 GLVISKELVELHNGSMSCQSRRGVGTRFMWTATFT 1222 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/36 (30%), Positives = 22/36 (61%) Frame = +2 Query: 458 LFDSTMANKISKLLTLLKMETLHRRRCKTRKWAAMK 565 +F+ T+ + + LTL +++ L +CKT K ++K Sbjct: 2115 VFEETLVDNLYHQLTLYQLQDLICSKCKTVKQWSLK 2150 >SPAC139.02c |oac1||anion transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/40 (27%), Positives = 18/40 (45%) Frame = -2 Query: 529 PMKRFHFQQSQQFGYLVRHRGIK*INVTFRKIYMFKFDFN 410 P KR + Q F + RH GI+ + Y+++ N Sbjct: 62 PSKRIYKSVGQAFSLIARHEGIRGLQRGLGTAYVYQICLN 101 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,008,344 Number of Sequences: 5004 Number of extensions: 60492 Number of successful extensions: 189 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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