BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120078.Seq (485 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schi... 91 1e-19 SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Sc... 89 3e-19 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 24 0.71 SPAC1782.11 |met14||adenylyl-sulfate kinase |Schizosaccharomyces... 26 2.6 SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyce... 25 6.1 >SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 90.6 bits (215), Expect = 1e-19 Identities = 43/82 (52%), Positives = 48/82 (58%) Frame = +1 Query: 4 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 183 M T KTRKLRGHVS AGG H R + DKYHPGYFGK+GMR Sbjct: 1 MPTHTSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60 Query: 184 FHFRKNKNFCPVLNLDKLWTLV 249 FH KN + P +NLD+LWTLV Sbjct: 61 FHLMKNPLWRPTVNLDRLWTLV 82 Score = 25.0 bits (52), Expect = 6.1 Identities = 16/64 (25%), Positives = 22/64 (34%) Frame = +3 Query: 255 QTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXXXXXXXDVGGAC 434 +TR KY PVIN++++ P PVI+ GG Sbjct: 85 ETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPDTPVIIQTRYVSRRAEEKIKQAGGVV 144 Query: 435 VLSA 446 L A Sbjct: 145 ELIA 148 >SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Schizosaccharomyces pombe|chr 3|||Manual Length = 148 Score = 89.0 bits (211), Expect = 3e-19 Identities = 42/82 (51%), Positives = 48/82 (58%) Frame = +1 Query: 4 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 183 M T KTRKLRGHVS AGG H R + DKYHPGYFGK+GMR Sbjct: 1 MPTHVSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60 Query: 184 FHFRKNKNFCPVLNLDKLWTLV 249 FH KN + P +NLD+LWTL+ Sbjct: 61 FHLMKNPLWRPTVNLDRLWTLL 82 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 24.2 bits (50), Expect(2) = 0.71 Identities = 7/15 (46%), Positives = 13/15 (86%) Frame = +1 Query: 211 CPVLNLDKLWTLVSD 255 C +L++D++W +VSD Sbjct: 776 CKLLSVDQIWAVVSD 790 Score = 22.2 bits (45), Expect(2) = 0.71 Identities = 9/30 (30%), Positives = 14/30 (46%) Frame = +1 Query: 148 HPGYFGKLGMRNFHFRKNKNFCPVLNLDKL 237 HP YF L F ++ C +L+ K+ Sbjct: 741 HPPYFDDLATTTFSSLLKRDECTILSFFKI 770 >SPAC1782.11 |met14||adenylyl-sulfate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 202 Score = 26.2 bits (55), Expect = 2.6 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = -2 Query: 325 YPALTILMTGTLPSGADAYFSLVCQRLMSKAYLSSKLDRNSCSF 194 +P +TI MTG SG + Q L+ + + +LD ++ F Sbjct: 23 HPGMTIWMTGLSASGKSTIACALEQYLLQRGVTTYRLDGDNVRF 66 >SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyces pombe|chr 2|||Manual Length = 406 Score = 25.0 bits (52), Expect = 6.1 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = -1 Query: 314 DNIDDGDLAIWSRCILQPRLS 252 DN+D+ DLA +S+ I +P L+ Sbjct: 2 DNLDEDDLAFFSKPIKKPPLN 22 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,765,094 Number of Sequences: 5004 Number of extensions: 33292 Number of successful extensions: 76 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 71 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 74 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 188065158 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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