BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120077.Seq (775 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g07410.1 68417.m01136 transducin family protein / WD-40 repea... 29 2.6 At1g78680.1 68414.m09171 gamma-glutamyl hydrolase (GGH1) / gamma... 29 2.6 At2g27770.1 68415.m03365 expressed protein 29 4.5 At4g09980.1 68417.m01634 methyltransferase MT-A70 family protein... 28 6.0 At1g30795.1 68414.m03765 hydroxyproline-rich glycoprotein family... 28 6.0 At4g38780.1 68417.m05491 splicing factor, putative strong simila... 28 7.9 At4g29810.1 68417.m04244 mitogen-activated protein kinase kinase... 28 7.9 >At4g07410.1 68417.m01136 transducin family protein / WD-40 repeat family protein contains 7 WD-40 repeats (PF00400) (2 weak); similar to Vegetatible incompatibility protein HET-E-1 (SP:Q00808) {Podospora anserina} Length = 815 Score = 29.5 bits (63), Expect = 2.6 Identities = 11/40 (27%), Positives = 27/40 (67%), Gaps = 1/40 (2%) Frame = -1 Query: 418 LQGLLHQNYDLPLIERENQPILF-GHITRSNTVILEKQWI 302 L+G ++ + ++ N P+LF GH+++++ +++EK W+ Sbjct: 759 LEGKSNERKNFEILP-SNHPVLFVGHLSKNSILVIEKPWM 797 >At1g78680.1 68414.m09171 gamma-glutamyl hydrolase (GGH1) / gamma-Glu-X carboxypeptidase / conjugase identical to SP|O65355 Gamma-glutamyl hydrolase precursor (EC 3.4.19.9) (Gamma-Glu-X carboxypeptidase) (Conjugase) (GH) {Arabidopsis thaliana} Length = 347 Score = 29.5 bits (63), Expect = 2.6 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = -1 Query: 388 LPLIERENQPILFGHITRSNTVILEKQWILEPIKGEVILRLNSITCFRTAVGE 230 +PLI E + ILF + N VIL W E + E++ ++ + R GE Sbjct: 95 IPLIFNEPEEILFQKLELVNGVILTGGWAKEGLYFEIVKKIFNKVLERNDAGE 147 >At2g27770.1 68415.m03365 expressed protein Length = 320 Score = 28.7 bits (61), Expect = 4.5 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +2 Query: 179 FYPPKFHFTPFGGNLPRLPDRCSKACDGIQPQNHFPL 289 FY K F G + + RC+K +G++ NH+P+ Sbjct: 191 FYSTKVRFVETGDS-HEIVIRCNKETEGLKQSNHYPV 226 >At4g09980.1 68417.m01634 methyltransferase MT-A70 family protein low similarity to SP|P25583 Karyogamy protein KAR4 {Saccharomyces cerevisiae}, (N6-adenosine)-methyltransferase [Mus musculus] GI:10179948; contains Pfam profile PF05063: MT-A70 (S-adenosylmethionine-binding subunit of human mRNA:m6A methyl-transferase (MTase)) Length = 775 Score = 28.3 bits (60), Expect = 6.0 Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Frame = -1 Query: 496 RTDG-RAKALLGGESEGTMEAAIKRSILQGLLHQNYDLPLIERENQP 359 R++G R+ LGG +G A+K G+ ++NYD +IE + +P Sbjct: 257 RSEGERSHGRLGGRKDGNRGEAVKALSSGGVSNENYD--VIEIQTKP 301 >At1g30795.1 68414.m03765 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR000694 Length = 109 Score = 28.3 bits (60), Expect = 6.0 Identities = 11/20 (55%), Positives = 15/20 (75%), Gaps = 1/20 (5%) Frame = +2 Query: 179 FYPP-KFHFTPFGGNLPRLP 235 F+PP +F PFGGN+P+ P Sbjct: 62 FFPPLQFAAAPFGGNIPQPP 81 >At4g38780.1 68417.m05491 splicing factor, putative strong similarity to splicing factor Prp8 [Homo sapiens] GI:3661610; contains Pfam profile PF01398: Mov34/MPN/PAD-1 family Length = 2332 Score = 27.9 bits (59), Expect = 7.9 Identities = 13/39 (33%), Positives = 24/39 (61%) Frame = -1 Query: 406 LHQNYDLPLIERENQPILFGHITRSNTVILEKQWILEPI 290 L +N +P + + +L+ HIT + T + E +W++EPI Sbjct: 97 LLENMPMPWEQVRDVKVLY-HITGAITFVNEVRWVVEPI 134 >At4g29810.1 68417.m04244 mitogen-activated protein kinase kinase (MAPKK) (MKK2) identical to MAP kinase kinase 2 [Arabidopsis thaliana] gi|3219267|dbj|BAA28828; mitogen-activated protein kinase kinase (MAPKK) family, PMID:12119167 Length = 363 Score = 27.9 bits (59), Expect = 7.9 Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 2/34 (5%) Frame = -1 Query: 439 AAIKRSILQGLLHQNYDLPLIERENQP--ILFGH 344 +AI R +LQGL++ ++D +I R+ +P +L H Sbjct: 169 SAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINH 202 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,874,070 Number of Sequences: 28952 Number of extensions: 338022 Number of successful extensions: 777 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 759 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 777 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1726528800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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