BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120053.Seq (771 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 79 9e-16 SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 75 9e-15 SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 71 1e-13 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 65 9e-12 SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc... 62 6e-11 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 59 6e-10 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 41 2e-04 SPAC11E3.10 |||VanZ-like family protein|Schizosaccharomyces pomb... 29 0.56 SPAC17G8.11c |||mannosyltransferase complex subunit |Schizosacch... 27 2.2 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 26 6.9 SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c... 26 6.9 SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|ch... 26 6.9 SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid... 25 9.1 >SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 767 Score = 78.6 bits (185), Expect = 9e-16 Identities = 37/93 (39%), Positives = 54/93 (58%), Gaps = 7/93 (7%) Frame = +1 Query: 1 FQNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI------ 162 ++ + +Y++ YSE DQ I+WFWE E E K + L F TG+ R+P+ G +D+ Sbjct: 657 WKKHTDYRS-YSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDLQGSDGP 715 Query: 163 -KIRIQPVADDRFFPVAHTCFNLLDLPRYKTKR 258 K I+ + P AHTCFN LDLP Y +K+ Sbjct: 716 RKFTIEKAGEPNKLPKAHTCFNRLDLPPYTSKK 748 >SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 786 Score = 75.4 bits (177), Expect = 9e-15 Identities = 38/92 (41%), Positives = 51/92 (55%), Gaps = 7/92 (7%) Frame = +1 Query: 1 FQNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI------ 162 ++++ EY+ Y TD I+WFWE ED+ K L F TG+ R+P+ G RD+ Sbjct: 676 WKSHTEYRT-YIATDPVIKWFWEIIAGWKNEDRSKLLQFATGTSRIPVNGFRDLQGSDGP 734 Query: 163 -KIRIQPVADDRFFPVAHTCFNLLDLPRYKTK 255 K I+ PVAHTCFN LDLP Y +K Sbjct: 735 RKFTIEKAGTPDQLPVAHTCFNRLDLPDYPSK 766 >SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharomyces pombe|chr 1|||Manual Length = 671 Score = 71.3 bits (167), Expect = 1e-13 Identities = 36/87 (41%), Positives = 49/87 (56%), Gaps = 7/87 (8%) Frame = +1 Query: 16 EYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI-------KIRI 174 +Y++ Y+ETD + WFWE E E K K L F TG+ R+P+ G +D+ K I Sbjct: 566 DYRS-YTETDDIVIWFWELLSEWSPEKKAKLLQFATGTSRLPLSGFKDMHGSDGPRKFTI 624 Query: 175 QPVADDRFFPVAHTCFNLLDLPRYKTK 255 + V P AHTCFN LD+P Y +K Sbjct: 625 EKVGHISQLPKAHTCFNRLDIPPYNSK 651 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 65.3 bits (152), Expect = 9e-12 Identities = 32/92 (34%), Positives = 50/92 (54%), Gaps = 8/92 (8%) Frame = +1 Query: 1 FQNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI------ 162 ++NN EY +GY+ + Q++WFW E++ K L F TG+ +VP+ G +++ Sbjct: 3115 WKNNTEY-HGYNVSSPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPLNGFKELEGMSGF 3173 Query: 163 -KIRI-QPVADDRFFPVAHTCFNLLDLPRYKT 252 + I + P +HTCFN LDLP Y T Sbjct: 3174 QRFNIHKSYGSLNRLPQSHTCFNQLDLPEYDT 3205 >SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 62.5 bits (145), Expect = 6e-11 Identities = 27/69 (39%), Positives = 42/69 (60%), Gaps = 1/69 (1%) Frame = +1 Query: 52 IRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIKIRIQPVA-DDRFFPVAHTCFNL 228 I WFW+ L+ +K FL+F+TGSDR+P G + ++RI + D P++HTCFN Sbjct: 719 ILWFWDLISHYSLKMQKLFLIFVTGSDRIPATGAHNFQLRISVLGPDSDQLPISHTCFNH 778 Query: 229 LDLPRYKTK 255 L + Y ++ Sbjct: 779 LCIWEYSSR 787 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 59.3 bits (137), Expect = 6e-10 Identities = 33/84 (39%), Positives = 41/84 (48%), Gaps = 4/84 (4%) Frame = +1 Query: 19 YKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIK----IRIQPVA 186 Y GY I FWE E EDK+ F+ F+T R PI G + + IR+ Sbjct: 928 YAGGYEPNSPTIVLFWEVLREFEEEDKRSFVKFVTSVARPPILGFKALMPSFCIRVNG-E 986 Query: 187 DDRFFPVAHTCFNLLDLPRYKTKR 258 D+ P A TC NLL LP Y TK+ Sbjct: 987 DETRLPTASTCVNLLKLPMYSTKQ 1010 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 41.1 bits (92), Expect = 2e-04 Identities = 22/84 (26%), Positives = 39/84 (46%), Gaps = 8/84 (9%) Frame = +1 Query: 25 NGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI--------KIRIQP 180 +GY+ I+ ++ ++++ FL F+TGS ++PI G + ++ P Sbjct: 1542 HGYTMESPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIGGFAGLNPPLTVVRRLNEPP 1601 Query: 181 VADDRFFPVAHTCFNLLDLPRYKT 252 D + P TC N L LP Y + Sbjct: 1602 YVPDDYLPSVMTCVNYLKLPEYSS 1625 >SPAC11E3.10 |||VanZ-like family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 29.5 bits (63), Expect = 0.56 Identities = 16/58 (27%), Positives = 33/58 (56%) Frame = +1 Query: 472 SLNKRVVSIDFVPS*ILCNLCFHGSITITIKSKYCHYFILNGILTHIFHQ*IFEFGKK 645 ++ K+V++I V I L F ++ + I K CH+F+ +LT +F+ +F+ ++ Sbjct: 10 AIRKKVLAIFIVLLIISAYLGFAPALPVPINDKVCHFFVF-FLLTLVFYW-VFDLSRR 65 >SPAC17G8.11c |||mannosyltransferase complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 356 Score = 27.5 bits (58), Expect = 2.2 Identities = 13/39 (33%), Positives = 17/39 (43%) Frame = +1 Query: 196 FFPVAHTCFNLLDLPRYKTKRGSSIIFCRPFSRRRASRW 312 FF V + F P + R +F PFS+ SRW Sbjct: 280 FFIVVYFIFGYALKPAARVSRTGRRVFSSPFSKTSPSRW 318 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 25.8 bits (54), Expect = 6.9 Identities = 14/33 (42%), Positives = 20/33 (60%) Frame = +1 Query: 55 RWFWETFHELPLEDKKKFLLFLTGSDRVPIQGM 153 R+ W+T H LP+E + L +TGSD V G+ Sbjct: 76 RFGWDT-HGLPVEHEIDKKLGITGSDDVMAMGI 107 >SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 25.8 bits (54), Expect = 6.9 Identities = 13/42 (30%), Positives = 19/42 (45%) Frame = +1 Query: 187 DDRFFPVAHTCFNLLDLPRYKTKRGSSIIFCRPFSRRRASRW 312 D+ F P H+ F+ R + + G S +SRR S W Sbjct: 84 DNIFHPQVHSSFHSRSASRQRRRSGLSRSNATRYSRRSLSDW 125 >SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 684 Score = 25.8 bits (54), Expect = 6.9 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = -1 Query: 624 LLVKYMCQDTI*NEIMTIFRFDRYCNG 544 L K +C D+I NEI+T+ R R NG Sbjct: 508 LTPKTICTDSI-NEIVTVIRAHRTANG 533 >SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp9|Schizosaccharomyces pombe|chr 3|||Manual Length = 610 Score = 25.4 bits (53), Expect = 9.1 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = +1 Query: 112 LFLTGSDRVPIQGMRDIKIRIQPVADDRFFPVAHTCFN 225 LF+TG+D ++ I + + D F P A CFN Sbjct: 104 LFITGADDKSLKVFDVESIDLVNIIDLEFLPKAICCFN 141 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,087,077 Number of Sequences: 5004 Number of extensions: 65165 Number of successful extensions: 173 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 159 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 371330890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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