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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120053.Seq
         (771 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    79   9e-16
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    75   9e-15
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    71   1e-13
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    65   9e-12
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc...    62   6e-11
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    59   6e-10
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    41   2e-04
SPAC11E3.10 |||VanZ-like family protein|Schizosaccharomyces pomb...    29   0.56 
SPAC17G8.11c |||mannosyltransferase complex subunit |Schizosacch...    27   2.2  
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    26   6.9  
SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c...    26   6.9  
SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|ch...    26   6.9  
SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid...    25   9.1  

>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 78.6 bits (185), Expect = 9e-16
 Identities = 37/93 (39%), Positives = 54/93 (58%), Gaps = 7/93 (7%)
 Frame = +1

Query: 1   FQNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI------ 162
           ++ + +Y++ YSE DQ I+WFWE   E   E K + L F TG+ R+P+ G +D+      
Sbjct: 657 WKKHTDYRS-YSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDLQGSDGP 715

Query: 163 -KIRIQPVADDRFFPVAHTCFNLLDLPRYKTKR 258
            K  I+   +    P AHTCFN LDLP Y +K+
Sbjct: 716 RKFTIEKAGEPNKLPKAHTCFNRLDLPPYTSKK 748


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 75.4 bits (177), Expect = 9e-15
 Identities = 38/92 (41%), Positives = 51/92 (55%), Gaps = 7/92 (7%)
 Frame = +1

Query: 1   FQNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI------ 162
           ++++ EY+  Y  TD  I+WFWE       ED+ K L F TG+ R+P+ G RD+      
Sbjct: 676 WKSHTEYRT-YIATDPVIKWFWEIIAGWKNEDRSKLLQFATGTSRIPVNGFRDLQGSDGP 734

Query: 163 -KIRIQPVADDRFFPVAHTCFNLLDLPRYKTK 255
            K  I+        PVAHTCFN LDLP Y +K
Sbjct: 735 RKFTIEKAGTPDQLPVAHTCFNRLDLPDYPSK 766


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 71.3 bits (167), Expect = 1e-13
 Identities = 36/87 (41%), Positives = 49/87 (56%), Gaps = 7/87 (8%)
 Frame = +1

Query: 16  EYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI-------KIRI 174
           +Y++ Y+ETD  + WFWE   E   E K K L F TG+ R+P+ G +D+       K  I
Sbjct: 566 DYRS-YTETDDIVIWFWELLSEWSPEKKAKLLQFATGTSRLPLSGFKDMHGSDGPRKFTI 624

Query: 175 QPVADDRFFPVAHTCFNLLDLPRYKTK 255
           + V      P AHTCFN LD+P Y +K
Sbjct: 625 EKVGHISQLPKAHTCFNRLDIPPYNSK 651


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 65.3 bits (152), Expect = 9e-12
 Identities = 32/92 (34%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
 Frame = +1

Query: 1    FQNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI------ 162
            ++NN EY +GY+ +  Q++WFW        E++ K L F TG+ +VP+ G +++      
Sbjct: 3115 WKNNTEY-HGYNVSSPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPLNGFKELEGMSGF 3173

Query: 163  -KIRI-QPVADDRFFPVAHTCFNLLDLPRYKT 252
             +  I +        P +HTCFN LDLP Y T
Sbjct: 3174 QRFNIHKSYGSLNRLPQSHTCFNQLDLPEYDT 3205


>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 807

 Score = 62.5 bits (145), Expect = 6e-11
 Identities = 27/69 (39%), Positives = 42/69 (60%), Gaps = 1/69 (1%)
 Frame = +1

Query: 52  IRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIKIRIQPVA-DDRFFPVAHTCFNL 228
           I WFW+      L+ +K FL+F+TGSDR+P  G  + ++RI  +  D    P++HTCFN 
Sbjct: 719 ILWFWDLISHYSLKMQKLFLIFVTGSDRIPATGAHNFQLRISVLGPDSDQLPISHTCFNH 778

Query: 229 LDLPRYKTK 255
           L +  Y ++
Sbjct: 779 LCIWEYSSR 787


>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 59.3 bits (137), Expect = 6e-10
 Identities = 33/84 (39%), Positives = 41/84 (48%), Gaps = 4/84 (4%)
 Frame = +1

Query: 19   YKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIK----IRIQPVA 186
            Y  GY      I  FWE   E   EDK+ F+ F+T   R PI G + +     IR+    
Sbjct: 928  YAGGYEPNSPTIVLFWEVLREFEEEDKRSFVKFVTSVARPPILGFKALMPSFCIRVNG-E 986

Query: 187  DDRFFPVAHTCFNLLDLPRYKTKR 258
            D+   P A TC NLL LP Y TK+
Sbjct: 987  DETRLPTASTCVNLLKLPMYSTKQ 1010


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 22/84 (26%), Positives = 39/84 (46%), Gaps = 8/84 (9%)
 Frame = +1

Query: 25   NGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI--------KIRIQP 180
            +GY+     I+       ++  ++++ FL F+TGS ++PI G   +        ++   P
Sbjct: 1542 HGYTMESPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIGGFAGLNPPLTVVRRLNEPP 1601

Query: 181  VADDRFFPVAHTCFNLLDLPRYKT 252
               D + P   TC N L LP Y +
Sbjct: 1602 YVPDDYLPSVMTCVNYLKLPEYSS 1625


>SPAC11E3.10 |||VanZ-like family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 162

 Score = 29.5 bits (63), Expect = 0.56
 Identities = 16/58 (27%), Positives = 33/58 (56%)
 Frame = +1

Query: 472 SLNKRVVSIDFVPS*ILCNLCFHGSITITIKSKYCHYFILNGILTHIFHQ*IFEFGKK 645
           ++ K+V++I  V   I   L F  ++ + I  K CH+F+   +LT +F+  +F+  ++
Sbjct: 10  AIRKKVLAIFIVLLIISAYLGFAPALPVPINDKVCHFFVF-FLLTLVFYW-VFDLSRR 65


>SPAC17G8.11c |||mannosyltransferase complex subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 13/39 (33%), Positives = 17/39 (43%)
 Frame = +1

Query: 196 FFPVAHTCFNLLDLPRYKTKRGSSIIFCRPFSRRRASRW 312
           FF V +  F     P  +  R    +F  PFS+   SRW
Sbjct: 280 FFIVVYFIFGYALKPAARVSRTGRRVFSSPFSKTSPSRW 318


>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = +1

Query: 55  RWFWETFHELPLEDKKKFLLFLTGSDRVPIQGM 153
           R+ W+T H LP+E +    L +TGSD V   G+
Sbjct: 76  RFGWDT-HGLPVEHEIDKKLGITGSDDVMAMGI 107


>SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 547

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 13/42 (30%), Positives = 19/42 (45%)
 Frame = +1

Query: 187 DDRFFPVAHTCFNLLDLPRYKTKRGSSIIFCRPFSRRRASRW 312
           D+ F P  H+ F+     R + + G S      +SRR  S W
Sbjct: 84  DNIFHPQVHSSFHSRSASRQRRRSGLSRSNATRYSRRSLSDW 125


>SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 684

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 13/27 (48%), Positives = 17/27 (62%)
 Frame = -1

Query: 624 LLVKYMCQDTI*NEIMTIFRFDRYCNG 544
           L  K +C D+I NEI+T+ R  R  NG
Sbjct: 508 LTPKTICTDSI-NEIVTVIRAHRTANG 533


>SPCC553.04 |cyp9||WD repeat containing cyclophilin family
           peptidyl-prolyl cis-trans isomerase
           Cyp9|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 610

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = +1

Query: 112 LFLTGSDRVPIQGMRDIKIRIQPVADDRFFPVAHTCFN 225
           LF+TG+D   ++      I +  + D  F P A  CFN
Sbjct: 104 LFITGADDKSLKVFDVESIDLVNIIDLEFLPKAICCFN 141


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,087,077
Number of Sequences: 5004
Number of extensions: 65165
Number of successful extensions: 173
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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