BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120047.Seq (809 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 29 0.59 SPAC1556.03 |azr1||serine/threonine protein phosphatase Azr1|Sch... 28 1.4 SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 3.2 SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 26 7.3 SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizo... 25 9.6 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 25 9.6 >SPAP27G11.15 |slx1||structure-specific endonuclease catalytic subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 29.5 bits (63), Expect = 0.59 Identities = 19/91 (20%), Positives = 43/91 (47%), Gaps = 2/91 (2%) Frame = +3 Query: 282 ECNV--KAVDSACMRCRRSFAVYPAVTYLHCGHSCLCTDCDETVNVDNTCPKCKSGIRYK 455 +CN+ + ++S +R F ++ +L C S T+ + + ++ C KCK +R++ Sbjct: 179 KCNLCYECIESDELRANCPFTDCNSINHLTCLASSFLTEECQVLPIEGMCTKCKRVLRWR 238 Query: 456 LKYKTL*HVALRNGDFRVVYLSPAQILNLNL 548 T+ +L + + +I++L L Sbjct: 239 EFLSTVFTTSLETDERDFESENRIEIIDLEL 269 >SPAC1556.03 |azr1||serine/threonine protein phosphatase Azr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 288 Score = 28.3 bits (60), Expect = 1.4 Identities = 17/47 (36%), Positives = 25/47 (53%) Frame = +3 Query: 432 CKSGIRYKLKYKTL*HVALRNGDFRVVYLSPAQILNLNLPLAYQKSV 572 C +G + L + LRNG + Y SPAQ+L N+P YQ ++ Sbjct: 137 CGNGKLHSLNLGDSGFLILRNG--AIHYASPAQVLQFNMP--YQLAI 179 >SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 27.1 bits (57), Expect = 3.2 Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 5/71 (7%) Frame = +3 Query: 276 NTECNVKAVDSACMRCRRSFAVYPAVTYLH---CGHS-CLCTDCDETVNVDN-TCPKCKS 440 + E V CM C + A+T + CGH+ C V+N +CP CK Sbjct: 180 SAETTVPQECGLCMMCVQRGDERVAITTPYTTDCGHTYCYACIMSRLKLVNNVSCPICKH 239 Query: 441 GIRYKLKYKTL 473 IR+ L +T+ Sbjct: 240 RIRFALPDQTM 250 >SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1639 Score = 25.8 bits (54), Expect = 7.3 Identities = 11/34 (32%), Positives = 21/34 (61%) Frame = -1 Query: 458 QFISNAAFTFRTRIVHVYRFVAIGAQTRMSAMQI 357 Q++SN + RT ++ + V+ +T+MSA Q+ Sbjct: 996 QYLSNMSHEIRTPLIGITGMVSFLLETQMSAEQL 1029 >SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizosaccharomyces pombe|chr 2|||Manual Length = 589 Score = 25.4 bits (53), Expect = 9.6 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +1 Query: 733 LERRLMVVMWHTRHFNVLCK 792 +ER++ H R FN+LCK Sbjct: 474 MERKISDAFQHGRFFNILCK 493 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 25.4 bits (53), Expect = 9.6 Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 5/78 (6%) Frame = +3 Query: 501 FRVVYLSPAQILNLNLPLAYQKSVLFASNSAKVNERIRRLARXXXXXXXS-----YFYHK 665 F+ ++LSP+ L L L +AY ++ NE++ L R S + K Sbjct: 546 FKYLHLSPSNRLQLWLKVAYCSALADLYELLMCNEKMSNLLRRVLKIMSSNDNIDELFLK 605 Query: 666 QFIKINFLTKKIINVYNK 719 + + IN L + + + Y++ Sbjct: 606 KCLNINLLLQHLSSEYSQ 623 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,040,930 Number of Sequences: 5004 Number of extensions: 56980 Number of successful extensions: 138 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 394431430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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