BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120036.Seq (735 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.69 SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 27 2.8 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 27 2.8 SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 26 4.8 SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 26 4.8 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 29.1 bits (62), Expect = 0.69 Identities = 31/105 (29%), Positives = 43/105 (40%), Gaps = 4/105 (3%) Frame = +1 Query: 400 KRGGGYFSFHC--WFSKITRTCINNFRLRRYNDLSPLQ--LGNVLYEPCTYDMQKLGSFI 567 K +F+F W +KI+ +CI F + + D + L V EP DM +G+ Sbjct: 816 KLPSNFFTFPASFWDAKIS-SCIPFFSNKLFIDQDFILGFLDLVASEPINVDMTDVGTQF 874 Query: 568 LVSLSARDKLICKSYRQNYVNFNGCHKAFSFSLLFVRLRIRFYCQ 702 + A Y YV GC F+L F L IR Y Q Sbjct: 875 VHIFHA------SLYTLYYVETTGCDGDALFNLTFAYLLIRIYLQ 913 >SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1372 Score = 27.1 bits (57), Expect = 2.8 Identities = 10/33 (30%), Positives = 20/33 (60%) Frame = +1 Query: 466 NFRLRRYNDLSPLQLGNVLYEPCTYDMQKLGSF 564 + RL R+N L+P+++ + C +D+ +L F Sbjct: 950 SIRLHRFNSLTPIEIAKKFFH-CDHDIMELVDF 981 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 27.1 bits (57), Expect = 2.8 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = -2 Query: 602 QINLSRAERDTKIKLPNFCISYVQG 528 Q+ R + T + PNFC+S++QG Sbjct: 700 QLERYRTDVRTVVNHPNFCLSFLQG 724 >SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1125 Score = 26.2 bits (55), Expect = 4.8 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -2 Query: 197 SNLC*TIFYLNVCYNHFSLNSVNSSTTKRFSN 102 SN ++ YLN NH NSV S+++ +F+N Sbjct: 410 SNHTVSLGYLNDRPNHSKKNSVTSTSSSQFNN 441 >SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 26.2 bits (55), Expect = 4.8 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = -2 Query: 179 IFYLNVCYNHFSLNSVNSSTTKRFSNCSTKTFVFVKKILLLQLLY 45 I+YL F + N ST +R N + TF+ + +++ Q+LY Sbjct: 162 IYYLQAREARF--RNTNESTNERVKNFAYLTFISLFVLVIWQILY 204 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,940,597 Number of Sequences: 5004 Number of extensions: 59431 Number of successful extensions: 137 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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