BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120008.Seq (681 letters) Database: tribolium 336 sequences; 122,585 total letters Searching.......................................................done Score E Sequences producing significant alignments: (bits) Value AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicas... 75 4e-16 AM292382-1|CAL23194.2| 670|Tribolium castaneum gustatory recept... 28 0.062 AM292340-1|CAL23152.1| 355|Tribolium castaneum gustatory recept... 23 2.3 >AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicase protein. Length = 580 Score = 75.4 bits (177), Expect = 4e-16 Identities = 40/82 (48%), Positives = 56/82 (68%), Gaps = 4/82 (4%) Frame = +2 Query: 257 GRLIDFLEKGTTNLQRCTYLVLDEADRMLDMGFEPQIRKII-EQIRP---DRQTLMWSAT 424 GRL DF+ +G + Y VLDEADRMLDMGF + +++ Q P +RQTLM+SAT Sbjct: 294 GRLKDFVNRGNVSFNSLKYFVLDEADRMLDMGFLGDVEEMLSHQSMPATGERQTLMFSAT 353 Query: 425 WPKEVKKLAEDYLGDYIQINIG 490 +P+EV++LA +L +YI I +G Sbjct: 354 FPEEVQQLAGKFLLNYIFIAVG 375 Score = 54.8 bits (126), Expect = 6e-10 Identities = 30/88 (34%), Positives = 48/88 (54%), Gaps = 4/88 (4%) Frame = +3 Query: 3 GSGKTLAYILPAIVHI--NNQPPIRRGD--GPIALVLAPTRELAQQIQQVAADFGHTSYV 170 GSGKT A++LP I ++ + PP + P+ ++++PTRELA QI F + S V Sbjct: 205 GSGKTAAFMLPIIHNLLSDKNPPNTENNCAQPVVVIMSPTRELAIQIADQGKKFAYNSTV 264 Query: 171 RNTCVFGGAPKREQARDLERGVEIVIAT 254 + ++GG Q + G I++AT Sbjct: 265 KVAVIYGGTSTNHQRGRILGGCHILVAT 292 Score = 24.2 bits (50), Expect = 1.0 Identities = 13/31 (41%), Positives = 20/31 (64%) Frame = +1 Query: 550 EKENKLNVLLQEIGQSQEPGAKTIIFVETKR 642 +K +KL +L++ P +T+IFVETKR Sbjct: 395 DKRSKLVSILEKA-----PNERTLIFVETKR 420 >AM292382-1|CAL23194.2| 670|Tribolium castaneum gustatory receptor candidate 61 protein. Length = 670 Score = 28.3 bits (60), Expect = 0.062 Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = +1 Query: 397 QTDFDVVSYLAQRSEETC*GLLGRLHSDQYRIITTFRNHNILQIVDIC-QEHEK 555 +T F V + + +R +L +L+ ++YR + + QIVDIC + H+K Sbjct: 469 ETQFVVANIILRRRFALINSILRKLYKNKYRKLIVDGKKSEEQIVDICIRSHDK 522 >AM292340-1|CAL23152.1| 355|Tribolium castaneum gustatory receptor candidate 19 protein. Length = 355 Score = 23.0 bits (47), Expect = 2.3 Identities = 19/56 (33%), Positives = 24/56 (42%), Gaps = 3/56 (5%) Frame = -3 Query: 673 LLMFLDMFSAFSWFQQKLLFLHQVLDFVQ---FLAIIHLIYFLFHVLDKYLQFEEC 515 LL + AF F LLFL + FV FL I+ Y F + +L F C Sbjct: 110 LLCVYYFYYAFIIFTVHLLFLLCIYYFVVPLFFLLCIYYFYCAFIIFTVHLLFLLC 165 Score = 21.4 bits (43), Expect = 7.1 Identities = 13/39 (33%), Positives = 18/39 (46%) Frame = -3 Query: 667 MFLDMFSAFSWFQQKLLFLHQVLDFVQFLAIIHLIYFLF 551 M L AF +F LLFL + F L + IY+ + Sbjct: 178 MHLLFCCAFIFFNMHLLFLLCLDYFTLHLLFLPCIYYFY 216 Database: tribolium Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 122,585 Number of sequences in database: 336 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 169,660 Number of Sequences: 336 Number of extensions: 3874 Number of successful extensions: 12 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 10 length of database: 122,585 effective HSP length: 55 effective length of database: 104,105 effective search space used: 17801955 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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