Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060991.seq
(689 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor |Schizosacc... 75 1e-14
SPCC330.11 |btb1||BTB/POZ domain protein Btb1|Schizosaccharomyce... 53 3e-08
SPBC3H7.06c |pof9||F-box protein Paf9|Schizosaccharomyces pombe|... 40 4e-04
SPAC10F6.04 |||RCC domain protein Ats1 |Schizosaccharomyces pomb... 36 0.004
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 29 0.63
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 27 1.9
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 27 1.9
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 27 1.9
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 26 5.9
SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 7.8
>SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 539
Score = 74.9 bits (176), Expect = 1e-14
Identities = 39/121 (32%), Positives = 63/121 (52%), Gaps = 4/121 (3%)
Frame = +2
Query: 260 IACGLQHSLAIDEWGQPFSWGSDSMGQLGNNLGAHAQDKPKFVKGLATKNVIQVACGAYH 439
+A G H +A+ G+ ++WG+ QLG + + + + LA KN+I V G+YH
Sbjct: 231 LATGTDHIIALTTTGKVYTWGNGQQFQLGRRMLERRRLQGLTPQPLALKNIISVGAGSYH 290
Query: 440 SVALTNSGDLYSWGANSYGQCGLELYQTRKCHL----QHISSLIGVPIALITCGNNHTFV 607
S A+ N G +Y+WG N QCG+E+ + + + +L G + IT G +HT
Sbjct: 291 SFAIDNKGRVYAWGLNITRQCGIEVEDEEEGAVITKPTLVDALEGYNVKSITGGEHHTLA 350
Query: 608 L 610
L
Sbjct: 351 L 351
Score = 54.4 bits (125), Expect = 1e-08
Identities = 34/87 (39%), Positives = 48/87 (55%), Gaps = 11/87 (12%)
Frame = +2
Query: 257 TIACGLQHSLAIDEWGQPFSWGSDSMGQLGNNLGAHAQDKPKFVKG----LATK------ 406
+I G H+LA+ E G+ +WG D QLG A + K KG L+T
Sbjct: 340 SITGGEHHTLALLEDGRVLAWGRDDRHQLGIPDNALPETVVKDEKGNNYYLSTPTIIPGL 399
Query: 407 -NVIQVACGAYHSVALTNSGDLYSWGA 484
NVIQV CG +H++A+T+ G +YSWG+
Sbjct: 400 TNVIQVVCGTHHNLAVTSDGKVYSWGS 426
Score = 49.2 bits (112), Expect = 6e-07
Identities = 38/142 (26%), Positives = 62/142 (43%), Gaps = 3/142 (2%)
Frame = +2
Query: 218 SSFSGNI*RLHNTTIACGLQHSLAIDEWGQPFSWGSD--SMGQLGNNLGAHAQDKPKFVK 391
S G + L T + C + AI + G F+WG+ S G LG + +P ++
Sbjct: 165 SKVEGALSHLRVTKVICSDNLTAAITDNGCCFTWGTFRCSDGVLGYSDSQKRTAEPTQMR 224
Query: 392 GLATKNVIQVACGAYHSVALTNSGDLYSWGANSYGQCGLELYQTRKCHLQHISSLIGVPI 571
+ Q+A G H +ALT +G +Y+WG Q G + + R+ L I
Sbjct: 225 ---LPEICQLATGTDHIIALTTTGKVYTWGNGQQFQLGRRMLERRRLQGLTPQPLALKNI 281
Query: 572 ALITCGNNHTFVL-SKSRCGFW 634
+ G+ H+F + +K R W
Sbjct: 282 ISVGAGSYHSFAIDNKGRVYAW 303
Score = 48.8 bits (111), Expect = 7e-07
Identities = 26/88 (29%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Frame = +2
Query: 257 TIACGLQHSLAIDEWGQPFSWGSDSMGQLGNNLGAHAQ----DKPKFVKGLATKNVIQVA 424
++ G HS AID G+ ++WG + Q G + + KP V L NV +
Sbjct: 283 SVGAGSYHSFAIDNKGRVYAWGLNITRQCGIEVEDEEEGAVITKPTLVDALEGYNVKSIT 342
Query: 425 CGAYHSVALTNSGDLYSWGANSYGQCGL 508
G +H++AL G + +WG + Q G+
Sbjct: 343 GGEHHTLALLEDGRVLAWGRDDRHQLGI 370
Score = 40.7 bits (91), Expect = 2e-04
Identities = 23/65 (35%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +3
Query: 30 GSQITDLVIKPTLSKWKESDHIQAV--AAGEFHSLYLSNSGHLYSCGNNDVGQLGRQTES 203
G + D+V +P L+ +D + V A G HS LS+ G +Y+ G ND LGR T+
Sbjct: 87 GEEEMDVVYRPRLNPILSTDKVGVVDLAVGGMHSAALSHDGRVYTWGVNDDYALGRLTKD 146
Query: 204 DNGKN 218
+N
Sbjct: 147 QKDEN 151
Score = 39.9 bits (89), Expect = 3e-04
Identities = 23/64 (35%), Positives = 36/64 (56%), Gaps = 5/64 (7%)
Frame = +2
Query: 311 FSWGSDSMGQLGNNLGAHAQD---KPKFVKGLATKNV--IQVACGAYHSVALTNSGDLYS 475
+ +GS SM +LG +G D +P+ L+T V + +A G HS AL++ G +Y+
Sbjct: 74 YVFGSGSMNELG--MGEEEMDVVYRPRLNPILSTDKVGVVDLAVGGMHSAALSHDGRVYT 131
Query: 476 WGAN 487
WG N
Sbjct: 132 WGVN 135
Score = 35.5 bits (78), Expect = 0.007
Identities = 16/47 (34%), Positives = 27/47 (57%)
Frame = +3
Query: 48 LVIKPTLSKWKESDHIQAVAAGEFHSLYLSNSGHLYSCGNNDVGQLG 188
++ KPTL E +++++ GE H+L L G + + G +D QLG
Sbjct: 323 VITKPTLVDALEGYNVKSITGGEHHTLALLEDGRVLAWGRDDRHQLG 369
Score = 35.5 bits (78), Expect = 0.007
Identities = 18/58 (31%), Positives = 26/58 (44%)
Frame = +2
Query: 260 IACGLQHSLAIDEWGQPFSWGSDSMGQLGNNLGAHAQDKPKFVKGLATKNVIQVACGA 433
+ CG H+LA+ G+ +SWGS ++G P V+ A K V GA
Sbjct: 405 VVCGTHHNLAVTSDGKVYSWGSAENYEVGQGDNDEDVAVPTLVRSKAIKEVAIRVAGA 462
Score = 32.7 bits (71), Expect = 0.051
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +3
Query: 90 HIQAVAAGEFHSLYLSNSGHLYSCGNNDVGQLGRQTESD 206
+I +V AG +HS + N G +Y+ G N Q G + E +
Sbjct: 280 NIISVGAGSYHSFAIDNKGRVYAWGLNITRQCGIEVEDE 318
Score = 31.5 bits (68), Expect = 0.12
Identities = 24/87 (27%), Positives = 43/87 (49%), Gaps = 13/87 (14%)
Frame = +2
Query: 260 IACGLQHSLAIDEWGQPFSWGSD---SMGQLGNNLGAHAQDK---------PKFVKG-LA 400
+A G HS A+ G+ ++WG + ++G+L + DK P V+G L+
Sbjct: 113 LAVGGMHSAALSHDGRVYTWGVNDDYALGRLTKDQKDENGDKVDNDLLEGTPSKVEGALS 172
Query: 401 TKNVIQVACGAYHSVALTNSGDLYSWG 481
V +V C + A+T++G ++WG
Sbjct: 173 HLRVTKVICSDNLTAAITDNGCCFTWG 199
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +3
Query: 102 VAAGEFHSLYLSNSGHLYSCGNNDVGQLGRQ 194
+A G H + L+ +G +Y+ GN QLGR+
Sbjct: 231 LATGTDHIIALTTTGKVYTWGNGQQFQLGRR 261
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/54 (27%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +3
Query: 102 VAAGEFHSLYLSNSGHLYSCGNNDVGQLGRQTESDNGKNPALV--ETFKDCTIR 257
V G H+L +++ G +YS G+ + ++G+ ++ P LV + K+ IR
Sbjct: 405 VVCGTHHNLAVTSDGKVYSWGSAENYEVGQGDNDEDVAVPTLVRSKAIKEVAIR 458
>SPCC330.11 |btb1||BTB/POZ domain protein Btb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1347
Score = 53.2 bits (122), Expect = 3e-08
Identities = 34/116 (29%), Positives = 59/116 (50%), Gaps = 5/116 (4%)
Frame = +2
Query: 278 HSLAIDEWGQPFSWGSDSMGQLGNNLGAHAQD-----KPKFVKGLATKNVIQVACGAYHS 442
HSLA+ ++G +SWG + G+LG + +D P+ + +I +A G +S
Sbjct: 258 HSLALTKFGSIYSWGKNGSGELGLSNDELKKDDPIQITPRRISAFKDYQIIGMAAGKSYS 317
Query: 443 VALTNSGDLYSWGANSYGQCGLELYQTRKCHLQHISSLIGVPIALITCGNNHTFVL 610
VA T++ D+YSWG N+ GQ G+ + + + ++ L+ P+ C T L
Sbjct: 318 VAWTDT-DIYSWGLNN-GQLGISDHISVVSTPRRVAGLLS-PVIHAVCTTRATICL 370
Score = 44.4 bits (100), Expect = 2e-05
Identities = 30/86 (34%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
Frame = +2
Query: 278 HSLAI-DEWGQP-FSWGSDSMGQLGNNLGAHAQDKPKFVKGLATKNVIQVACGAYHSVAL 451
HS+ + DE Q + G + G++G N P + G+ K VIQ++ HS+AL
Sbjct: 206 HSVVLTDEPSQNVYVCGIGAGGRIGFNTDVQYNFIP--IPGIIHK-VIQISVSHTHSLAL 262
Query: 452 TNSGDLYSWGANSYGQCGLELYQTRK 529
T G +YSWG N G+ GL + +K
Sbjct: 263 TKFGSIYSWGKNGSGELGLSNDELKK 288
Score = 29.9 bits (64), Expect = 0.36
Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
Frame = +3
Query: 60 PTLSKWKESDHIQAVAAGEFHSLYLSN--SGHLYSCGNNDVGQLGRQTE 200
PT + D I V A +FHS+ L++ S ++Y CG G++G T+
Sbjct: 186 PTSGQLFSRDKILDVQASKFHSVVLTDEPSQNVYVCGIGAGGRIGFNTD 234
Score = 29.5 bits (63), Expect = 0.48
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +3
Query: 102 VAAGEFHSLYLSNSGHLYSCGNNDVGQLGRQTESDNGKNP 221
++ HSL L+ G +YS G N G+LG + +P
Sbjct: 252 ISVSHTHSLALTKFGSIYSWGKNGSGELGLSNDELKKDDP 291
>SPBC3H7.06c |pof9||F-box protein Paf9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 467
Score = 39.5 bits (88), Expect = 4e-04
Identities = 22/68 (32%), Positives = 32/68 (47%), Gaps = 3/68 (4%)
Frame = +2
Query: 302 GQPFSWGSDSMGQLGN--NLGAHAQDKPKFVKGLATKNVIQVACGAYHSVALTNSGDLYS 475
G +SWG LG N + +D+P + G ++ IQV G Y L +G+LY
Sbjct: 78 GYAYSWGQQIRNYLGRVVNTNQYPRDRPGALTGKDVRSTIQVQVGGYGMYLLNENGNLYV 137
Query: 476 WGA-NSYG 496
G N+ G
Sbjct: 138 TGVPNNVG 145
Score = 28.7 bits (61), Expect = 0.84
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +2
Query: 341 LGNNLGAHAQDKPKFVKGLATKNVIQVACGAYHSVALTNSGDLYSWG 481
+G+ D P L + + Q+A G +H + LT+ ++SWG
Sbjct: 316 MGHANTKQVDDDPVIHDFLQNRGIKQLAHGDHHHLYLTSDHSIWSWG 362
>SPAC10F6.04 |||RCC domain protein Ats1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 351
Score = 36.3 bits (80), Expect = 0.004
Identities = 31/126 (24%), Positives = 58/126 (46%), Gaps = 9/126 (7%)
Frame = +2
Query: 260 IACGLQHSLAID-EWGQPFSWGSDSMGQLGNNLGAHAQDKPKF-VKGLATKN-VIQVACG 430
+ CG + S+ + + + S G G+LG +++Q + + L K +I ++ G
Sbjct: 94 LTCGWEFSVIVHADRRRVCSCGEGLSGELGQGNRSNSQGLREIDIPYLDDKEFIIDISAG 153
Query: 431 AYHSVALTNSGDLYSWGANSYGQCGLELYQT-RKC----HLQHISSLI-GVPIALITCGN 592
H + +TN G+LY G GQ G + +T K ++H +++ GV + +
Sbjct: 154 LRHWICVTNEGNLYGCGDGRKGQLGPVVMKTVNKVSFLGRIEHAQAVVCGVQFSAVLQET 213
Query: 593 NHTFVL 610
H VL
Sbjct: 214 GHVIVL 219
Score = 34.7 bits (76), Expect = 0.013
Identities = 21/67 (31%), Positives = 33/67 (49%)
Frame = +2
Query: 314 SWGSDSMGQLGNNLGAHAQDKPKFVKGLATKNVIQVACGAYHSVALTNSGDLYSWGANSY 493
S GS+ QLG N P+ V + + +++CG H++ L L++ G N
Sbjct: 4 SLGSNGNFQLGLNNDEDVYS-PQIVP--FDRAIEKISCGGNHTLLLDEDSQLWACGDNRK 60
Query: 494 GQCGLEL 514
GQCG E+
Sbjct: 61 GQCGYEV 67
Score = 33.9 bits (74), Expect = 0.022
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +3
Query: 81 ESDHIQAVAAGEFHSLYLSNSGHLYSCGNNDVGQLG 188
+ + I ++AG H + ++N G+LY CG+ GQLG
Sbjct: 143 DKEFIIDISAGLRHWICVTNEGNLYGCGDGRKGQLG 178
Score = 31.5 bits (68), Expect = 0.12
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 93 IQAVAAGEFHSLYLSNSGHLYSCGNNDVGQLGRQTE 200
I+ ++ G H+L L L++CG+N GQ G + +
Sbjct: 33 IEKISCGGNHTLLLDEDSQLWACGDNRKGQCGYEVK 68
Score = 28.3 bits (60), Expect = 1.1
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +2
Query: 260 IACGLQHSLAIDEWGQPFSWGSDSMGQLG 346
I+CG H+L +DE Q ++ G + GQ G
Sbjct: 36 ISCGGNHTLLLDEDSQLWACGDNRKGQCG 64
Score = 27.9 bits (59), Expect = 1.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +2
Query: 407 NVIQVACGAYHSVALTNSGDLYSWGANSYG 496
+++Q+A G H++ T G + +G N +G
Sbjct: 281 DIVQIASGTEHNILRTKKGSVLIYGWNEHG 310
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 29.1 bits (62), Expect = 0.63
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 389 KGLATKNVIQVACGAY-HSVALTNSGDLYSWGANS 490
KGL+ K++I CGA+ H L ++ + Y G NS
Sbjct: 230 KGLSGKDIIVSLCGAFNHDEVLNHAIEFYGEGLNS 264
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 27.5 bits (58), Expect = 1.9
Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 1/33 (3%)
Frame = +3
Query: 78 KESDHIQAVAAGEFHSLYLSNSGHL-YSCGNND 173
KE HI V G S + +GHL Y CG D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGID 35
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 27.5 bits (58), Expect = 1.9
Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 1/33 (3%)
Frame = +3
Query: 78 KESDHIQAVAAGEFHSLYLSNSGHL-YSCGNND 173
KE HI V G S + +GHL Y CG D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGID 35
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 27.5 bits (58), Expect = 1.9
Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 1/33 (3%)
Frame = +3
Query: 78 KESDHIQAVAAGEFHSLYLSNSGHL-YSCGNND 173
KE HI V G S + +GHL Y CG D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGID 35
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 25.8 bits (54), Expect = 5.9
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +2
Query: 584 CGNNHTFVLSKSRCGFW 634
CG TFV S +RC W
Sbjct: 1675 CGCEETFVTSLARCSLW 1691
>SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 591
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +2
Query: 407 NVIQVACGAYHSVALTNSGDLYSWGANSYG 496
+++ AC A AL +G +Y W A S G
Sbjct: 123 SIVTAACLAEICSALPAAGSIYLWAAESAG 152
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,112,897
Number of Sequences: 5004
Number of extensions: 66173
Number of successful extensions: 194
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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