BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060989.seq (676 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 73 4e-14 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 72 9e-14 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 71 2e-13 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 46 5e-06 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 44 2e-05 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 3e-04 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 37 0.002 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 37 0.002 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.46 SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 26 4.3 SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1||... 25 7.6 SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyce... 25 7.6 SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyce... 25 10.0 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 10.0 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 72.9 bits (171), Expect = 4e-14 Identities = 46/122 (37%), Positives = 70/122 (57%), Gaps = 10/122 (8%) Frame = +1 Query: 142 IFYRYLALCNGVLG--PLRFVFRRYRADTE*FRQISYKFREIWIIEFFAPWCGHCKSLVP 315 +F +L +GV G P+ F +++ FR+ K + ++ F+APWCG+CK LVP Sbjct: 11 LFLACFSLVSGVFGYSPM-FGSNTIELNSKNFRKF-VKAKGPSLVVFYAPWCGYCKKLVP 68 Query: 316 EYKKAARALKGIVKVGAL--DADEHRSVSQKYGVTGFPTIK-IFTGSK-----HTPYQGQ 471 Y+K A L ++ V A+ DAD++R+V +Y V GFPTIK ++ SK T Y G Sbjct: 69 TYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGD 128 Query: 472 RT 477 R+ Sbjct: 129 RS 130 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 71.7 bits (168), Expect = 9e-14 Identities = 35/95 (36%), Positives = 52/95 (54%), Gaps = 4/95 (4%) Frame = +1 Query: 265 IIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGALDADEHRSVSQKYGVTGFPTIKIF 438 +IEF+A WCGHCKSL P Y++ + V +G +DAD H V+ KY +TGFPT+ F Sbjct: 43 LIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWF 102 Query: 439 --TGSKHTPYQGQRTAEGFVEAALKEPRRRHMKIL 537 GS+ Y R + + ++ + KI+ Sbjct: 103 PPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIV 137 Score = 60.1 bits (139), Expect = 3e-10 Identities = 29/82 (35%), Positives = 45/82 (54%), Gaps = 5/82 (6%) Frame = +1 Query: 265 IIEFFAPWCGHCKSLVPEYKKAARALKG--IVKVGALDADEHRSVSQKYGVTGFPTIKIF 438 ++EF+A WCG+CK L P Y+ + K V++ ++AD + + + V FPTIK F Sbjct: 162 LVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFF 221 Query: 439 -TGSKHTP--YQGQRTAEGFVE 495 K P Y+G R+ E +E Sbjct: 222 PKDDKDKPELYEGDRSLESLIE 243 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 70.5 bits (165), Expect = 2e-13 Identities = 30/87 (34%), Positives = 52/87 (59%), Gaps = 2/87 (2%) Frame = +1 Query: 256 EIWIIEFFAPWCGHCKSLVPEYKKAARAL-KGIVKVGALDADEHRSVSQKYGVTGFPTIK 432 ++ +++F+APWCGHCK+L PEY+ AA L K + + +D E + +Y + G+PT+ Sbjct: 40 KVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTLN 99 Query: 433 IF-TGSKHTPYQGQRTAEGFVEAALKE 510 +F G + + Y G R + V+ K+ Sbjct: 100 VFKNGKQISQYSGPRKHDALVKYMRKQ 126 Score = 61.3 bits (142), Expect = 1e-10 Identities = 34/78 (43%), Positives = 45/78 (57%), Gaps = 5/78 (6%) Frame = +1 Query: 265 IIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGALDADEHRSVSQKYGVTGFPTIKIF 438 ++EF+APWCGHCK+L P Y+K A V V +DA E+ +S ++GFPTI F Sbjct: 377 LVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATEN-DIS--VSISGFPTIMFF 433 Query: 439 -TGSKHTP--YQGQRTAE 483 K P Y+G RT E Sbjct: 434 KANDKVNPVRYEGDRTLE 451 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 46.0 bits (104), Expect = 5e-06 Identities = 20/61 (32%), Positives = 37/61 (60%) Frame = +1 Query: 256 EIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKI 435 ++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT+ + Sbjct: 36 KVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPTMVL 94 Query: 436 F 438 F Sbjct: 95 F 95 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 44.0 bits (99), Expect = 2e-05 Identities = 24/99 (24%), Positives = 53/99 (53%), Gaps = 1/99 (1%) Frame = +1 Query: 214 ADTE*FRQISYKFREIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSV 393 +D+ F+ I + + + +++FFA WCG CK++ P++++ + + +D D+ + Sbjct: 6 SDSSEFKSIVCQDKLV-VVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQLSEI 63 Query: 394 SQKYGVTGFPTIKIF-TGSKHTPYQGQRTAEGFVEAALK 507 + + GV P+ ++ G K G A+ +EA++K Sbjct: 64 AAEAGVHAMPSFFLYKNGEKIEEIVGANPAK--LEASIK 100 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%) Frame = +1 Query: 268 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTG 444 ++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83 Query: 445 SK 450 K Sbjct: 84 GK 85 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 37.1 bits (82), Expect = 0.002 Identities = 16/72 (22%), Positives = 31/72 (43%) Frame = +1 Query: 253 REIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIK 432 +E W I+F++ C C + + A ++G + V ++ + ++Y + FPT Sbjct: 298 KEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQYSIQYFPTFL 357 Query: 433 IFTGSKHTPYQG 468 F Y G Sbjct: 358 FFKEEAFVEYVG 369 Score = 29.5 bits (63), Expect = 0.46 Identities = 15/62 (24%), Positives = 27/62 (43%), Gaps = 3/62 (4%) Frame = +1 Query: 262 WIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DADEHRSVSQKYGVTGFPTIK 432 W I+++ P CG CK L P + K V+ + D + +S + PT+ Sbjct: 45 WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLY 104 Query: 433 IF 438 ++ Sbjct: 105 LY 106 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 37.1 bits (82), Expect = 0.002 Identities = 19/77 (24%), Positives = 40/77 (51%), Gaps = 1/77 (1%) Frame = +1 Query: 223 E*FRQISYKFRE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQ 399 E F++I +E I ++ F+APW CK + + + A+ K V + ++A++ +++ Sbjct: 9 EQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEKFSDIAE 67 Query: 400 KYGVTGFPTIKIFTGSK 450 + V P + G+K Sbjct: 68 SFDVNAVPLFVLIHGAK 84 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 29.5 bits (63), Expect = 0.46 Identities = 13/60 (21%), Positives = 24/60 (40%) Frame = +1 Query: 289 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 468 C C + R +K+ ++ DE + + + + FPT ++F G Y G Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268 >SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 472 Score = 26.2 bits (55), Expect = 4.3 Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 1/26 (3%) Frame = +1 Query: 577 CHYS-NRQQLQRTGLDSDDLWLVEFY 651 C++S N+ Q ++T LD D+ VEFY Sbjct: 194 CYHSLNKLQSKKTDLDDDERSSVEFY 219 >SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 654 Score = 25.4 bits (53), Expect = 7.6 Identities = 13/47 (27%), Positives = 27/47 (57%) Frame = +2 Query: 362 VP*MLMNIEVYHRNMESLASPQLRYSQGASTHHIKVRGQQKDLLKLL 502 VP +L+N+E +H+ ++ A+P + +Q H + ++D+ LL Sbjct: 134 VPPLLLNVESHHKVLDMCAAPGSKTAQLLEALHKPTK--KEDITTLL 178 >SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 138 Score = 25.4 bits (53), Expect = 7.6 Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 1/51 (1%) Frame = +1 Query: 295 HCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGV-TGFPTIKIFTG 444 HC KAAR L I++ ++ E S+SQK + PT+ I G Sbjct: 69 HCTYSQVRGPKAARVLSEILRNRITESKEKLSLSQKEKLFQNLPTVYILHG 119 >SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 459 Score = 25.0 bits (52), Expect = 10.0 Identities = 12/42 (28%), Positives = 17/42 (40%) Frame = -3 Query: 386 LCSSASKAPTFTMPFRALAAFLYSGTRLLQCPHHGAKNSMIQ 261 LC K T + + F +CPH G KN+ +Q Sbjct: 37 LCMECGKNGTTKLLLTVIPYFREVVLMSFECPHCGFKNAQVQ 78 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 25.0 bits (52), Expect = 10.0 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +1 Query: 280 APWCGHCKSLVPEYKKAAR 336 A WC HC ++P +K AR Sbjct: 484 ANWCCHCGYILPLGRKTAR 502 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,808,166 Number of Sequences: 5004 Number of extensions: 57451 Number of successful extensions: 167 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 309878492 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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